Intrinsic reaction-cycle time scale of Na+,K+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes
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Interaction between integral membrane proteins and the lipid-bilayer component of biological membranes is expected to mutually infl uence the proteins and the membrane. We present quantitative evidence of a manifestation of the lipid-protein interactions in liposomal membranes, reconstituted with actively pumping Na+,K+-ATPase, in terms of nonequilibrium shape fluctuations that contain a relaxation time, τ, which is robust and independent of the speci fi c fl uctuation modes of the membrane. In the case of pumping Na+-ions, analysis of the flicker-noise temporal correlation spectrum of the liposomes leads to τ ≃ 0.5 s, comparing favorably with an intrinsic reaction-cycle time of about 0.4 s from enzymology.
Originalsprog | Engelsk |
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Tidsskrift | Proceedings of the National Academy of Sciences of the United States of America |
Vol/bind | 109 |
Udgave nummer | 45 |
Sider (fra-til) | 18442-18446 |
Antal sider | 5 |
ISSN | 0027-8424 |
DOI | |
Status | Udgivet - 2012 |
Eksternt udgivet | Ja |
ID: 230975063