Metabolic footprinting for investigation of antifungal properties of Lactobacillus paracasei

Research output: Contribution to journalJournal articlepeer-review

Standard

Metabolic footprinting for investigation of antifungal properties of Lactobacillus paracasei. / Honoré, Anders Hans; Aunsbjerg, Stina Dissing; Ebrahimi, Parvaneh; Thorsen, Michael; Benfeldt, Connie; Knøchel, Susanne; Skov, Thomas.

In: Analytical and Bioanalytical Chemistry, Vol. 408, No. 1, 2016, p. 83-96.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Honoré, AH, Aunsbjerg, SD, Ebrahimi, P, Thorsen, M, Benfeldt, C, Knøchel, S & Skov, T 2016, 'Metabolic footprinting for investigation of antifungal properties of Lactobacillus paracasei', Analytical and Bioanalytical Chemistry, vol. 408, no. 1, pp. 83-96. https://doi.org/10.1007/s00216-015-9103-6

APA

Honoré, A. H., Aunsbjerg, S. D., Ebrahimi, P., Thorsen, M., Benfeldt, C., Knøchel, S., & Skov, T. (2016). Metabolic footprinting for investigation of antifungal properties of Lactobacillus paracasei. Analytical and Bioanalytical Chemistry, 408(1), 83-96. https://doi.org/10.1007/s00216-015-9103-6

Vancouver

Honoré AH, Aunsbjerg SD, Ebrahimi P, Thorsen M, Benfeldt C, Knøchel S et al. Metabolic footprinting for investigation of antifungal properties of Lactobacillus paracasei. Analytical and Bioanalytical Chemistry. 2016;408(1):83-96. https://doi.org/10.1007/s00216-015-9103-6

Author

Honoré, Anders Hans ; Aunsbjerg, Stina Dissing ; Ebrahimi, Parvaneh ; Thorsen, Michael ; Benfeldt, Connie ; Knøchel, Susanne ; Skov, Thomas. / Metabolic footprinting for investigation of antifungal properties of Lactobacillus paracasei. In: Analytical and Bioanalytical Chemistry. 2016 ; Vol. 408, No. 1. pp. 83-96.

Bibtex

@article{d2fc1c5c3d134b9398e6ffb9679e6ed6,
title = "Metabolic footprinting for investigation of antifungal properties of Lactobacillus paracasei",
abstract = "Lactic acid bacteria with antifungal properties are applied for biopreservation of food. In order to further our understanding of their antifungal mechanism, there is an ongoing search for bioactive molecules. With a focus on the metabolites formed, bioassay-guided fractionation and comprehensive screening have identified compounds as antifungal. Although these are active, the compounds have been found in concentrations that are too low to account for the observed antifungal effect. It has been hypothesized that the formation of metabolites and consumption of nutrients during bacterial fermentations form the basis for the antifungal effect, i.e., the composition of the exometabolome. To build a more comprehensive view of the chemical changes induced by bacterial fermentation and the effects on mold growth, a strategy for correlating the exometabolomic profiles with mold growth was applied. The antifungal properties were assessed by measuring mold growth of two Penicillium strains on cell-free ferments of three strains of Lactobacillus paracasei pre-fermented in a chemically defined medium. Exometabolomic profiling was performed by reversed-phase liquid chromatography in combination with mass spectrometry in electrospray positive and negative modes. By multivariate data analysis, the three strains of Lb. paracasei were readily distinguished by the relative difference of their exometabolomes. The relative differences correlated with the relative growth of the two Penicillium strains. Metabolic footprinting proved to be a supplement to bioassay-guided fractionation for investigation of antifungal properties of bacterial ferments. Additionally, three previously identified and three novel antifungal metabolites from Lb. paracasei and their potential precursors were detected and assigned using the strategy.",
author = "Honor{\'e}, {Anders Hans} and Aunsbjerg, {Stina Dissing} and Parvaneh Ebrahimi and Michael Thorsen and Connie Benfeldt and Susanne Kn{\o}chel and Thomas Skov",
year = "2016",
doi = "10.1007/s00216-015-9103-6",
language = "English",
volume = "408",
pages = "83--96",
journal = "Analytical and Bioanalytical Chemistry",
issn = "1618-2642",
publisher = "Springer",
number = "1",

}

RIS

TY - JOUR

T1 - Metabolic footprinting for investigation of antifungal properties of Lactobacillus paracasei

AU - Honoré, Anders Hans

AU - Aunsbjerg, Stina Dissing

AU - Ebrahimi, Parvaneh

AU - Thorsen, Michael

AU - Benfeldt, Connie

AU - Knøchel, Susanne

AU - Skov, Thomas

PY - 2016

Y1 - 2016

N2 - Lactic acid bacteria with antifungal properties are applied for biopreservation of food. In order to further our understanding of their antifungal mechanism, there is an ongoing search for bioactive molecules. With a focus on the metabolites formed, bioassay-guided fractionation and comprehensive screening have identified compounds as antifungal. Although these are active, the compounds have been found in concentrations that are too low to account for the observed antifungal effect. It has been hypothesized that the formation of metabolites and consumption of nutrients during bacterial fermentations form the basis for the antifungal effect, i.e., the composition of the exometabolome. To build a more comprehensive view of the chemical changes induced by bacterial fermentation and the effects on mold growth, a strategy for correlating the exometabolomic profiles with mold growth was applied. The antifungal properties were assessed by measuring mold growth of two Penicillium strains on cell-free ferments of three strains of Lactobacillus paracasei pre-fermented in a chemically defined medium. Exometabolomic profiling was performed by reversed-phase liquid chromatography in combination with mass spectrometry in electrospray positive and negative modes. By multivariate data analysis, the three strains of Lb. paracasei were readily distinguished by the relative difference of their exometabolomes. The relative differences correlated with the relative growth of the two Penicillium strains. Metabolic footprinting proved to be a supplement to bioassay-guided fractionation for investigation of antifungal properties of bacterial ferments. Additionally, three previously identified and three novel antifungal metabolites from Lb. paracasei and their potential precursors were detected and assigned using the strategy.

AB - Lactic acid bacteria with antifungal properties are applied for biopreservation of food. In order to further our understanding of their antifungal mechanism, there is an ongoing search for bioactive molecules. With a focus on the metabolites formed, bioassay-guided fractionation and comprehensive screening have identified compounds as antifungal. Although these are active, the compounds have been found in concentrations that are too low to account for the observed antifungal effect. It has been hypothesized that the formation of metabolites and consumption of nutrients during bacterial fermentations form the basis for the antifungal effect, i.e., the composition of the exometabolome. To build a more comprehensive view of the chemical changes induced by bacterial fermentation and the effects on mold growth, a strategy for correlating the exometabolomic profiles with mold growth was applied. The antifungal properties were assessed by measuring mold growth of two Penicillium strains on cell-free ferments of three strains of Lactobacillus paracasei pre-fermented in a chemically defined medium. Exometabolomic profiling was performed by reversed-phase liquid chromatography in combination with mass spectrometry in electrospray positive and negative modes. By multivariate data analysis, the three strains of Lb. paracasei were readily distinguished by the relative difference of their exometabolomes. The relative differences correlated with the relative growth of the two Penicillium strains. Metabolic footprinting proved to be a supplement to bioassay-guided fractionation for investigation of antifungal properties of bacterial ferments. Additionally, three previously identified and three novel antifungal metabolites from Lb. paracasei and their potential precursors were detected and assigned using the strategy.

U2 - 10.1007/s00216-015-9103-6

DO - 10.1007/s00216-015-9103-6

M3 - Journal article

C2 - 26573172

VL - 408

SP - 83

EP - 96

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

SN - 1618-2642

IS - 1

ER -

ID: 148093184