Visualisation of morphological interactionof diamond and silver nanoparticles with Salmonella enteritidis and Listeria Monocytogenes

Research output: Contribution to journalJournal articleResearchpeer-review

  • Ewa Sawosz
  • Chwalibog, André
  • Katarzyna Mitura
  • Stanislaw Mitura
  • Jacek Szeliga
  • Tomasz Niemiec
  • Marlena Rupiewicz
  • Marta Grodzik
  • Aleksandra Sokolowska
Currently, medicine intensively searches for methods to transport drugs to a target (sick) point within the body. The objective of the present investigation was to evaluate morphological characteristics of the assembles of silver or diamond nanoparticles with Salmonella Enteritidis (G-) or Listeria monocytogenes (G+), to reveal possibilities of constructing nanoparticle-bacteria vehicles. Diamond nanoparticles (nano-D) were produced by the detonation method. Hydrocolloids of silver nanoparticles (nano-Ag) were produced by electric non-explosive patented method. Hydrocolloids of nanoparticles (200 microl) were added to bacteria suspension (200 microl) in the following order: nano-D + Salmonella E.; nano-D + Listeria monocytogenes; nano-Ag + Salmonella E; nano-Ag + Listeria monocytogenes. Samples were inspected by transmission electron microscopy. Visualisation of nanoparticles and bacteria interaction showed harmful effects of both nanoparticles on bacteria morphology. The most spectacular effect of nano-D were strong links between nano-D packages and the flagella of Salmonella E. Nano-Ag were closely attached to Listeria monocytogenes but not to Salmonella E. There was no evidence of entering nano-Ag inside Listeria monocytogenes but smaller particles were placed inside Salmonella E. The ability of nano-D to attach to the flagella and the ability of nano-Ag to penetrate inside bacteria cells can be utilized to design nano-bacteria vehicles, being carriers for active substances attached to nanoparticles.
Original languageEnglish
JournalJournal of Nanoscience and Nanotechnology
Issue number9
Pages (from-to)7635-7641
Number of pages7
Publication statusPublished - 2011

ID: 35084888