Variation in the gut microbiota of laboratory mice is related to both genetic and environmental factors

Research output: Contribution to journalJournal articleResearchpeer-review

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Variation in the gut microbiota of laboratory mice is related to both genetic and environmental factors. / Hufeldt, Majbritt Ravn; Nielsen, Dennis Sandris; Vogensen, Finn Kvist; Midtvedt, Tore; Hansen, Axel Jacob Kornerup.

In: Comparative Medicine, Vol. 60, No. 5, 2010, p. 336-342.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Hufeldt, MR, Nielsen, DS, Vogensen, FK, Midtvedt, T & Hansen, AJK 2010, 'Variation in the gut microbiota of laboratory mice is related to both genetic and environmental factors', Comparative Medicine, vol. 60, no. 5, pp. 336-342.

APA

Hufeldt, M. R., Nielsen, D. S., Vogensen, F. K., Midtvedt, T., & Hansen, A. J. K. (2010). Variation in the gut microbiota of laboratory mice is related to both genetic and environmental factors. Comparative Medicine, 60(5), 336-342.

Vancouver

Hufeldt MR, Nielsen DS, Vogensen FK, Midtvedt T, Hansen AJK. Variation in the gut microbiota of laboratory mice is related to both genetic and environmental factors. Comparative Medicine. 2010;60(5):336-342.

Author

Hufeldt, Majbritt Ravn ; Nielsen, Dennis Sandris ; Vogensen, Finn Kvist ; Midtvedt, Tore ; Hansen, Axel Jacob Kornerup. / Variation in the gut microbiota of laboratory mice is related to both genetic and environmental factors. In: Comparative Medicine. 2010 ; Vol. 60, No. 5. pp. 336-342.

Bibtex

@article{a0f83c354c5f4234a7d101c0ed375fb1,
title = "Variation in the gut microbiota of laboratory mice is related to both genetic and environmental factors",
abstract = "During recent years, the composition of the gut microbiota (GM) has received increasing attention as a factor in the development of experimental inflammatory disease in animal models. Because increased variation in the GM might lead to increased variation in disease parameters, determining and reducing GM variation between laboratory animals may provide more consistent models. Both genetic and environmental aspects influence the composition of the GM and may vary between laboratory animal breeding centers and within an individual breeding center. This study investigated the variation in cecal microbiota in 8-wk-old NMRI and C57BL/6 mice by using denaturing gradient gel electrophoresis to profile PCR-derived amplicons from bacterial 16S rRNA genes. Comparison of the cecal microbiotas revealed that the similarity index of the inbred C57BL/6Sca strain was 10{\%} higher than that of the outbred Sca:NMRI stock. Comparing C57BL/6 mice from 2 vendors revealed significant differences in the microbial profile, whereas the profiles of C57BL/6Sca mice raised in separate rooms within the same breeding center were not significantly different. Furthermore, housing in individually ventilated cages did not lead to intercage variation. These results show that denaturing gradient gel electrophoresis is a simple tool that can be used to characterize the gut microbiota of mice. Including such characterizations in future quality-control programs may increase the reproducibility of mouse studies.",
keywords = "Former LIFE faculty",
author = "Hufeldt, {Majbritt Ravn} and Nielsen, {Dennis Sandris} and Vogensen, {Finn Kvist} and Tore Midtvedt and Hansen, {Axel Jacob Kornerup}",
year = "2010",
language = "English",
volume = "60",
pages = "336--342",
journal = "Comparative Medicine",
issn = "1532-0820",
publisher = "American Association for Laboratory Animal Science",
number = "5",

}

RIS

TY - JOUR

T1 - Variation in the gut microbiota of laboratory mice is related to both genetic and environmental factors

AU - Hufeldt, Majbritt Ravn

AU - Nielsen, Dennis Sandris

AU - Vogensen, Finn Kvist

AU - Midtvedt, Tore

AU - Hansen, Axel Jacob Kornerup

PY - 2010

Y1 - 2010

N2 - During recent years, the composition of the gut microbiota (GM) has received increasing attention as a factor in the development of experimental inflammatory disease in animal models. Because increased variation in the GM might lead to increased variation in disease parameters, determining and reducing GM variation between laboratory animals may provide more consistent models. Both genetic and environmental aspects influence the composition of the GM and may vary between laboratory animal breeding centers and within an individual breeding center. This study investigated the variation in cecal microbiota in 8-wk-old NMRI and C57BL/6 mice by using denaturing gradient gel electrophoresis to profile PCR-derived amplicons from bacterial 16S rRNA genes. Comparison of the cecal microbiotas revealed that the similarity index of the inbred C57BL/6Sca strain was 10% higher than that of the outbred Sca:NMRI stock. Comparing C57BL/6 mice from 2 vendors revealed significant differences in the microbial profile, whereas the profiles of C57BL/6Sca mice raised in separate rooms within the same breeding center were not significantly different. Furthermore, housing in individually ventilated cages did not lead to intercage variation. These results show that denaturing gradient gel electrophoresis is a simple tool that can be used to characterize the gut microbiota of mice. Including such characterizations in future quality-control programs may increase the reproducibility of mouse studies.

AB - During recent years, the composition of the gut microbiota (GM) has received increasing attention as a factor in the development of experimental inflammatory disease in animal models. Because increased variation in the GM might lead to increased variation in disease parameters, determining and reducing GM variation between laboratory animals may provide more consistent models. Both genetic and environmental aspects influence the composition of the GM and may vary between laboratory animal breeding centers and within an individual breeding center. This study investigated the variation in cecal microbiota in 8-wk-old NMRI and C57BL/6 mice by using denaturing gradient gel electrophoresis to profile PCR-derived amplicons from bacterial 16S rRNA genes. Comparison of the cecal microbiotas revealed that the similarity index of the inbred C57BL/6Sca strain was 10% higher than that of the outbred Sca:NMRI stock. Comparing C57BL/6 mice from 2 vendors revealed significant differences in the microbial profile, whereas the profiles of C57BL/6Sca mice raised in separate rooms within the same breeding center were not significantly different. Furthermore, housing in individually ventilated cages did not lead to intercage variation. These results show that denaturing gradient gel electrophoresis is a simple tool that can be used to characterize the gut microbiota of mice. Including such characterizations in future quality-control programs may increase the reproducibility of mouse studies.

KW - Former LIFE faculty

M3 - Journal article

VL - 60

SP - 336

EP - 342

JO - Comparative Medicine

JF - Comparative Medicine

SN - 1532-0820

IS - 5

ER -

ID: 32375329