USE OF FLOW CYTOMETRY FOR RAPID ESTIMATION OF INTRACELLULAR EVENTS IN BREWING YEASTS
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USE OF FLOW CYTOMETRY FOR RAPID ESTIMATION OF INTRACELLULAR EVENTS IN BREWING YEASTS. / Jespersen, Lene; Jakobsen, Mogens.
In: Journal of the Institute of Brewing, Vol. 100, No. 6, 1994, p. 399-403.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - USE OF FLOW CYTOMETRY FOR RAPID ESTIMATION OF INTRACELLULAR EVENTS IN BREWING YEASTS
AU - Jespersen, Lene
AU - Jakobsen, Mogens
PY - 1994
Y1 - 1994
N2 - A flow cytometric method based on staining with fluorescein diacetate (FDA) for rapid measurement of the fluorescence intensity (FI) of individual brewing yeast cells is reported. Distinct changes in the FI of yeast populations during the course of both laboratory scale and brewery fermentations were observed. The changes followed a characteristic pattern defined as the FI‐profile. The FI‐profile was related to the rate of attenuation as well as changes in yeast glycogen and ergosterol contents. The results obtained suggest that flow cytometry can be applied for estimation of intracellular events in brewing yeasts and prediction of their performance. 1994 The Institute of Brewing & Distilling
AB - A flow cytometric method based on staining with fluorescein diacetate (FDA) for rapid measurement of the fluorescence intensity (FI) of individual brewing yeast cells is reported. Distinct changes in the FI of yeast populations during the course of both laboratory scale and brewery fermentations were observed. The changes followed a characteristic pattern defined as the FI‐profile. The FI‐profile was related to the rate of attenuation as well as changes in yeast glycogen and ergosterol contents. The results obtained suggest that flow cytometry can be applied for estimation of intracellular events in brewing yeasts and prediction of their performance. 1994 The Institute of Brewing & Distilling
KW - Brewing yeasts
KW - ergosterol
KW - fermentation
KW - flow cytometry
KW - fluorescein diacetate
KW - glycogen
UR - http://www.scopus.com/inward/record.url?scp=84987309982&partnerID=8YFLogxK
U2 - 10.1002/j.2050-0416.1994.tb00837.x
DO - 10.1002/j.2050-0416.1994.tb00837.x
M3 - Journal article
AN - SCOPUS:84987309982
VL - 100
SP - 399
EP - 403
JO - Journal of the Federated Institutes of Brewing
JF - Journal of the Federated Institutes of Brewing
SN - 0046-9750
IS - 6
ER -
ID: 247157178