Ultrastructural modeling of small angle scattering from photosynthetic membranes

Research output: Contribution to journalJournal articlepeer-review

Standard

Ultrastructural modeling of small angle scattering from photosynthetic membranes. / Jakubauskas, Dainius; Kowalewska, Łucja; Sokolova, Anna V.; Garvey, Christopher J.; Mortensen, Kell; Jensen, Poul Erik; Kirkensgaard, Jacob J.K.

In: Scientific Reports, Vol. 9, 19405, 01.12.2019.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Jakubauskas, D, Kowalewska, Ł, Sokolova, AV, Garvey, CJ, Mortensen, K, Jensen, PE & Kirkensgaard, JJK 2019, 'Ultrastructural modeling of small angle scattering from photosynthetic membranes', Scientific Reports, vol. 9, 19405. https://doi.org/10.1038/s41598-019-55423-0

APA

Jakubauskas, D., Kowalewska, Ł., Sokolova, A. V., Garvey, C. J., Mortensen, K., Jensen, P. E., & Kirkensgaard, J. J. K. (2019). Ultrastructural modeling of small angle scattering from photosynthetic membranes. Scientific Reports, 9, [19405]. https://doi.org/10.1038/s41598-019-55423-0

Vancouver

Jakubauskas D, Kowalewska Ł, Sokolova AV, Garvey CJ, Mortensen K, Jensen PE et al. Ultrastructural modeling of small angle scattering from photosynthetic membranes. Scientific Reports. 2019 Dec 1;9. 19405. https://doi.org/10.1038/s41598-019-55423-0

Author

Jakubauskas, Dainius ; Kowalewska, Łucja ; Sokolova, Anna V. ; Garvey, Christopher J. ; Mortensen, Kell ; Jensen, Poul Erik ; Kirkensgaard, Jacob J.K. / Ultrastructural modeling of small angle scattering from photosynthetic membranes. In: Scientific Reports. 2019 ; Vol. 9.

Bibtex

@article{0e797a7586db47d49551bb1503a5a10d,
title = "Ultrastructural modeling of small angle scattering from photosynthetic membranes",
abstract = "The last decade has seen a range of studies using non-invasive neutron and X-ray techniques to probe the ultrastructure of a variety of photosynthetic membrane systems. A common denominator in this work is the lack of an explicitly formulated underlying structural model, ultimately leading to ambiguity in the data interpretation. Here we formulate and implement a full mathematical model of the scattering from a stacked double bilayer membrane system taking instrumental resolution and polydispersity into account. We validate our model by direct simulation of scattering patterns from 3D structural models. Most importantly, we demonstrate that the full scattering curves from three structurally typical cyanobacterial thylakoid membrane systems measured in vivo can all be described within this framework. The model provides realistic estimates of key structural parameters in the thylakoid membrane, in particular the overall stacking distance and how this is divided between membranes, lumen and cytoplasmic liquid. Finally, from fitted scattering length densities it becomes clear that the protein content in the inner lumen has to be lower than in the outer cytoplasmic liquid and we extract the first quantitative measure of the luminal protein content in a living cyanobacteria.",
author = "Dainius Jakubauskas and {\L}ucja Kowalewska and Sokolova, {Anna V.} and Garvey, {Christopher J.} and Kell Mortensen and Jensen, {Poul Erik} and Kirkensgaard, {Jacob J.K.}",
year = "2019",
month = dec,
day = "1",
doi = "10.1038/s41598-019-55423-0",
language = "English",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Ultrastructural modeling of small angle scattering from photosynthetic membranes

AU - Jakubauskas, Dainius

AU - Kowalewska, Łucja

AU - Sokolova, Anna V.

AU - Garvey, Christopher J.

AU - Mortensen, Kell

AU - Jensen, Poul Erik

AU - Kirkensgaard, Jacob J.K.

PY - 2019/12/1

Y1 - 2019/12/1

N2 - The last decade has seen a range of studies using non-invasive neutron and X-ray techniques to probe the ultrastructure of a variety of photosynthetic membrane systems. A common denominator in this work is the lack of an explicitly formulated underlying structural model, ultimately leading to ambiguity in the data interpretation. Here we formulate and implement a full mathematical model of the scattering from a stacked double bilayer membrane system taking instrumental resolution and polydispersity into account. We validate our model by direct simulation of scattering patterns from 3D structural models. Most importantly, we demonstrate that the full scattering curves from three structurally typical cyanobacterial thylakoid membrane systems measured in vivo can all be described within this framework. The model provides realistic estimates of key structural parameters in the thylakoid membrane, in particular the overall stacking distance and how this is divided between membranes, lumen and cytoplasmic liquid. Finally, from fitted scattering length densities it becomes clear that the protein content in the inner lumen has to be lower than in the outer cytoplasmic liquid and we extract the first quantitative measure of the luminal protein content in a living cyanobacteria.

AB - The last decade has seen a range of studies using non-invasive neutron and X-ray techniques to probe the ultrastructure of a variety of photosynthetic membrane systems. A common denominator in this work is the lack of an explicitly formulated underlying structural model, ultimately leading to ambiguity in the data interpretation. Here we formulate and implement a full mathematical model of the scattering from a stacked double bilayer membrane system taking instrumental resolution and polydispersity into account. We validate our model by direct simulation of scattering patterns from 3D structural models. Most importantly, we demonstrate that the full scattering curves from three structurally typical cyanobacterial thylakoid membrane systems measured in vivo can all be described within this framework. The model provides realistic estimates of key structural parameters in the thylakoid membrane, in particular the overall stacking distance and how this is divided between membranes, lumen and cytoplasmic liquid. Finally, from fitted scattering length densities it becomes clear that the protein content in the inner lumen has to be lower than in the outer cytoplasmic liquid and we extract the first quantitative measure of the luminal protein content in a living cyanobacteria.

U2 - 10.1038/s41598-019-55423-0

DO - 10.1038/s41598-019-55423-0

M3 - Journal article

C2 - 31852917

AN - SCOPUS:85076931261

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 19405

ER -

ID: 234077239