The permeability and the effect of acyl-chain length for phospholipid bilayers containing cholesterol: theory and experiment
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The permeability and the effect of acyl-chain length for phospholipid bilayers containing cholesterol : theory and experiment. / Corvera, E.; Mouritsen, O. G.; Singer, M. A.; Zuckermann, M. J.
In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1107, No. 2, 1992, p. 261-270.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The permeability and the effect of acyl-chain length for phospholipid bilayers containing cholesterol
T2 - theory and experiment
AU - Corvera, E.
AU - Mouritsen, O. G.
AU - Singer, M. A.
AU - Zuckermann, M. J.
PY - 1992
Y1 - 1992
N2 - The model of Cruzeiro-Hansson et al. (Biochim. Biophys. Acta (1989) 979, 166-1176) for lipid-cholesterol bilayers at low cholesterol concentrations is used to predict the thermodynamic properties and the passive ion permeability of lipid bilayers as a function of acyl-chain length and cholesterol concentration. Numerical simulations based on the Monte Carlo method are used to determine the equilibrium state of the system near the main gel-fluid phase transition. The permeability is calculated using an ansatz which relates the passive permeability to the amount of interfaces formed in the bilayer when cholesterol is present. The model predicts at low cholesterol contents an increase in the membrane permeability in the transition region both for increasing cholesterol concentration and for decreasing chain length at a given value of the reduced temperature. This is in contrast to the case of lipid bilayers containing high cholesterol concentrations where the cholesterol strongly surpresses the permeability. Experimental results for the Na+ permeability of C15PC and DPPC (C16PC) bilayers containing cholesterol are presented which confirm the theoretical predictions at low cholesterol concentrations.
AB - The model of Cruzeiro-Hansson et al. (Biochim. Biophys. Acta (1989) 979, 166-1176) for lipid-cholesterol bilayers at low cholesterol concentrations is used to predict the thermodynamic properties and the passive ion permeability of lipid bilayers as a function of acyl-chain length and cholesterol concentration. Numerical simulations based on the Monte Carlo method are used to determine the equilibrium state of the system near the main gel-fluid phase transition. The permeability is calculated using an ansatz which relates the passive permeability to the amount of interfaces formed in the bilayer when cholesterol is present. The model predicts at low cholesterol contents an increase in the membrane permeability in the transition region both for increasing cholesterol concentration and for decreasing chain length at a given value of the reduced temperature. This is in contrast to the case of lipid bilayers containing high cholesterol concentrations where the cholesterol strongly surpresses the permeability. Experimental results for the Na+ permeability of C15PC and DPPC (C16PC) bilayers containing cholesterol are presented which confirm the theoretical predictions at low cholesterol concentrations.
KW - Cholesterol
KW - Lipid bilayer
KW - Passive permeability
KW - Permeability
KW - Phase transition
KW - Thermal fluctuation
U2 - 10.1016/0005-2736(92)90413-G
DO - 10.1016/0005-2736(92)90413-G
M3 - Journal article
C2 - 1504071
AN - SCOPUS:0026780558
VL - 1107
SP - 261
EP - 270
JO - B B A - Biomembranes
JF - B B A - Biomembranes
SN - 0005-2736
IS - 2
ER -
ID: 236892134