Systematic relationship between phospholipase A2 activity and dynamic lipid bilayer microheterogeneity

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Systematic relationship between phospholipase A2 activity and dynamic lipid bilayer microheterogeneity. / Hønger, T.; Jørgensen, K.; Biltonen, R. L.; Mouritsen, O. G.

In: Biochemistry, Vol. 35, No. 28, 10.08.1996, p. 9003-9006.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hønger, T, Jørgensen, K, Biltonen, RL & Mouritsen, OG 1996, 'Systematic relationship between phospholipase A2 activity and dynamic lipid bilayer microheterogeneity', Biochemistry, vol. 35, no. 28, pp. 9003-9006. https://doi.org/10.1021/bi960866a

APA

Hønger, T., Jørgensen, K., Biltonen, R. L., & Mouritsen, O. G. (1996). Systematic relationship between phospholipase A2 activity and dynamic lipid bilayer microheterogeneity. Biochemistry, 35(28), 9003-9006. https://doi.org/10.1021/bi960866a

Vancouver

Hønger T, Jørgensen K, Biltonen RL, Mouritsen OG. Systematic relationship between phospholipase A2 activity and dynamic lipid bilayer microheterogeneity. Biochemistry. 1996 Aug 10;35(28):9003-9006. https://doi.org/10.1021/bi960866a

Author

Hønger, T. ; Jørgensen, K. ; Biltonen, R. L. ; Mouritsen, O. G. / Systematic relationship between phospholipase A2 activity and dynamic lipid bilayer microheterogeneity. In: Biochemistry. 1996 ; Vol. 35, No. 28. pp. 9003-9006.

Bibtex

@article{f7385b23149949308e0ec0149c24214a,
title = "Systematic relationship between phospholipase A2 activity and dynamic lipid bilayer microheterogeneity",
abstract = "A standing hypothesis in membrane biology implies that the collective physical properties of the lipid bilayer component of biological membranes can modulate the activity of membrane-associated proteins. We provide strong support for this hypothesis by exploring a model system, phospholipase A2 catalyzed hydrolysis of one-component phospholipid vesicles. For vesicles of lipids with different chain lengths we observe, as a function of temperature and chain length, a systematic variation of the characteristic lag time for the onset of rapid phospholipase A2 hydrolysis. These results, combined with theoretical results obtained from computer simulation of the gel-to-fluid phase transition in the unhydrolyzed lipid bilayers, enable us to demonstrate a strong correlation between the lag time and the degree of bilayer microheterogeneity in the phase transition region. Insight into the nature of this correlation suggests rational ways of modulating enzyme activity by modifying the physical properties of the lipid bilayer.",
author = "T. H{\o}nger and K. J{\o}rgensen and Biltonen, {R. L.} and Mouritsen, {O. G.}",
year = "1996",
month = aug,
day = "10",
doi = "10.1021/bi960866a",
language = "English",
volume = "35",
pages = "9003--9006",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "28",

}

RIS

TY - JOUR

T1 - Systematic relationship between phospholipase A2 activity and dynamic lipid bilayer microheterogeneity

AU - Hønger, T.

AU - Jørgensen, K.

AU - Biltonen, R. L.

AU - Mouritsen, O. G.

PY - 1996/8/10

Y1 - 1996/8/10

N2 - A standing hypothesis in membrane biology implies that the collective physical properties of the lipid bilayer component of biological membranes can modulate the activity of membrane-associated proteins. We provide strong support for this hypothesis by exploring a model system, phospholipase A2 catalyzed hydrolysis of one-component phospholipid vesicles. For vesicles of lipids with different chain lengths we observe, as a function of temperature and chain length, a systematic variation of the characteristic lag time for the onset of rapid phospholipase A2 hydrolysis. These results, combined with theoretical results obtained from computer simulation of the gel-to-fluid phase transition in the unhydrolyzed lipid bilayers, enable us to demonstrate a strong correlation between the lag time and the degree of bilayer microheterogeneity in the phase transition region. Insight into the nature of this correlation suggests rational ways of modulating enzyme activity by modifying the physical properties of the lipid bilayer.

AB - A standing hypothesis in membrane biology implies that the collective physical properties of the lipid bilayer component of biological membranes can modulate the activity of membrane-associated proteins. We provide strong support for this hypothesis by exploring a model system, phospholipase A2 catalyzed hydrolysis of one-component phospholipid vesicles. For vesicles of lipids with different chain lengths we observe, as a function of temperature and chain length, a systematic variation of the characteristic lag time for the onset of rapid phospholipase A2 hydrolysis. These results, combined with theoretical results obtained from computer simulation of the gel-to-fluid phase transition in the unhydrolyzed lipid bilayers, enable us to demonstrate a strong correlation between the lag time and the degree of bilayer microheterogeneity in the phase transition region. Insight into the nature of this correlation suggests rational ways of modulating enzyme activity by modifying the physical properties of the lipid bilayer.

UR - http://www.scopus.com/inward/record.url?scp=0029893610&partnerID=8YFLogxK

U2 - 10.1021/bi960866a

DO - 10.1021/bi960866a

M3 - Journal article

C2 - 8703902

AN - SCOPUS:0029893610

VL - 35

SP - 9003

EP - 9006

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 28

ER -

ID: 236888194