Survival of Listeria monocytogenes in simulated gastrointestinal system and transcriptional profiling of stress- and adhesion-related genes
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Survival of Listeria monocytogenes in simulated gastrointestinal system and transcriptional profiling of stress- and adhesion-related genes. / Jiang, Lingli; Olesen, Inger; Andersen, Thomas; Fang, Weihuan; Jespersen, Lene.
In: Foodborne Pathogens and Disease, Vol. 7, No. 3, 2010, p. 267-274.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Survival of Listeria monocytogenes in simulated gastrointestinal system and transcriptional profiling of stress- and adhesion-related genes
AU - Jiang, Lingli
AU - Olesen, Inger
AU - Andersen, Thomas
AU - Fang, Weihuan
AU - Jespersen, Lene
PY - 2010
Y1 - 2010
N2 - Food ingestion is the major route of exposure to the important human pathogen Listeria monocytogenes. An in vitrogastrointestinal model was used to (1) compare the survival rates of L. monocytogenes strains of serotypes 1=2a,1=2c, and 4b; and (2) examine the transcription of stress- and adhesion-related genes after exposure to the conditions similar to those encountered in the mouth, stomach, and small intestine. None of the L. monocytogenes strains investigated could survive in the gastric juice at pH 2.5 or 3.0. Their survival increased at higher pH (3.5 and 4.0) in the gastric stress. Relative survival of L. monocytogenes serotypes 4b and 1=2a strains were higher than that of serotype 1=2c, suggesting that pathogenicity might be related to the viability in the gastrointestinal tract.The transcription levels of prfA and the general stress-related genes clpC, clpE, and clpP were upregulated afterpassing through the simulated gastrointestinal tract, whereas that of the adhesion-related gene ami was downregulated. Taken together, this study revealed that L. monocytogenes strains enhanced the expression of stressrelated genes and decreased the transcription of adhesion-related gene in order to survive in the diverse microenvironments
AB - Food ingestion is the major route of exposure to the important human pathogen Listeria monocytogenes. An in vitrogastrointestinal model was used to (1) compare the survival rates of L. monocytogenes strains of serotypes 1=2a,1=2c, and 4b; and (2) examine the transcription of stress- and adhesion-related genes after exposure to the conditions similar to those encountered in the mouth, stomach, and small intestine. None of the L. monocytogenes strains investigated could survive in the gastric juice at pH 2.5 or 3.0. Their survival increased at higher pH (3.5 and 4.0) in the gastric stress. Relative survival of L. monocytogenes serotypes 4b and 1=2a strains were higher than that of serotype 1=2c, suggesting that pathogenicity might be related to the viability in the gastrointestinal tract.The transcription levels of prfA and the general stress-related genes clpC, clpE, and clpP were upregulated afterpassing through the simulated gastrointestinal tract, whereas that of the adhesion-related gene ami was downregulated. Taken together, this study revealed that L. monocytogenes strains enhanced the expression of stressrelated genes and decreased the transcription of adhesion-related gene in order to survive in the diverse microenvironments
U2 - 10.1089/fpd.2009.0361
DO - 10.1089/fpd.2009.0361
M3 - Journal article
C2 - 19899961
VL - 7
SP - 267
EP - 274
JO - Foodborne Pathogens and Disease
JF - Foodborne Pathogens and Disease
SN - 1535-3141
IS - 3
ER -
ID: 15862941