Search for proteomic markers for stunning stress and stress-induced textural tenderization in silver carp (Hypophthalmichthys molitrix) fillets using label-free strategy
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Search for proteomic markers for stunning stress and stress-induced textural tenderization in silver carp (Hypophthalmichthys molitrix) fillets using label-free strategy. / Zhang, Longteng; Li, Qian; Hong, Hui; Luo, Yongkang; Lametsch, René.
In: Food Research International, Vol. 137, 109678, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Search for proteomic markers for stunning stress and stress-induced textural tenderization in silver carp (Hypophthalmichthys molitrix) fillets using label-free strategy
AU - Zhang, Longteng
AU - Li, Qian
AU - Hong, Hui
AU - Luo, Yongkang
AU - Lametsch, René
PY - 2020
Y1 - 2020
N2 - A differential proteome analysis using label-free strategy was conducted to elucidate the stunning stress-induced proteome changes and its relationship to textural softening in silver carp (Hypophthalmichthys molitrix) fillets. Compared with the muscle proteome of fillets stunned by percussion, ice/water and gill cut stunning resulted in 43 differentially abundant proteins (DAPs) that were involved in muscle contraction, energy metabolism, antioxidant defense, protein turnover, etc., and gill cut-stunned fillets showed the most significant proteome changes with 28 up- and down-regulated DAPs. In addition, textural softening was more obvious in ice/water- and gill cut-treated fillets and was closely correlated to 21 DAPs including fast muscle troponin T, fragments of myosin, superoxide dismutase [Cu-Zn], ribosomal protein L38, isoform 2 of heat shock cognate 70, two subunits of proteasome, etc., and those DAPs were selected as potential proteomic markers to trace the accelerated textural softening in stunning-stressed fillets.
AB - A differential proteome analysis using label-free strategy was conducted to elucidate the stunning stress-induced proteome changes and its relationship to textural softening in silver carp (Hypophthalmichthys molitrix) fillets. Compared with the muscle proteome of fillets stunned by percussion, ice/water and gill cut stunning resulted in 43 differentially abundant proteins (DAPs) that were involved in muscle contraction, energy metabolism, antioxidant defense, protein turnover, etc., and gill cut-stunned fillets showed the most significant proteome changes with 28 up- and down-regulated DAPs. In addition, textural softening was more obvious in ice/water- and gill cut-treated fillets and was closely correlated to 21 DAPs including fast muscle troponin T, fragments of myosin, superoxide dismutase [Cu-Zn], ribosomal protein L38, isoform 2 of heat shock cognate 70, two subunits of proteasome, etc., and those DAPs were selected as potential proteomic markers to trace the accelerated textural softening in stunning-stressed fillets.
KW - Differentially abundant proteins
KW - Label-free proteomics
KW - Proteomic markers
KW - Stunning stress
KW - Textural softening
U2 - 10.1016/j.foodres.2020.109678
DO - 10.1016/j.foodres.2020.109678
M3 - Journal article
C2 - 33233255
AN - SCOPUS:85091217710
VL - 137
JO - Food Research International
JF - Food Research International
SN - 0963-9969
M1 - 109678
ER -
ID: 249768051