Screening effect of PEG on avidin binding to liposome surface receptors
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Screening effect of PEG on avidin binding to liposome surface receptors. / Kaasgaard, Thomas; Mouritsen, Ole G.; Jørgensen, Kent.
In: International Journal of Pharmaceutics, Vol. 214, No. 1-2, 19.02.2001, p. 63-65.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Screening effect of PEG on avidin binding to liposome surface receptors
AU - Kaasgaard, Thomas
AU - Mouritsen, Ole G.
AU - Jørgensen, Kent
PY - 2001/2/19
Y1 - 2001/2/19
N2 - This study investigates the screening effect of poly(ethylene glycol)-phospholipids (PE-PEG) on the interaction of avidin with PEGylated liposomes containing surface-bound biotin ligands. The influence of grafting density and lipopolymer chain length is examined. A simple fluorescence assay involving a receptor-mediated fluorescence increase of BODIPY-labeled avidin upon binding to biotinylated lipids is employed to study the screening effect of submicellar concentrations of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine-N-[poly(ethylene glycol)-2000] (PE-PEG2000) and 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine-N-[poly(ethylene glycol)-5000] (PE-PEG5000) incorporated into 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) liposomes. The results show that incorporation of lipopolymers into DPPC lipid bilayers reduces binding of avidin to the biotinylated liposomes, and it is found that the screening effect of PE-PEG5000 is stronger than that for PE-PEG2000. Thus, the results reveal that both the grafting density and the polymer length of the PE-PEG lipopolymers are of importance for the ability of water-soluble macromolecules to reach the surface of PEG liposomes. Furthermore, it is found that none of the lipopolymers completely prevents avidin from reaching the surface-bound biotin ligands.
AB - This study investigates the screening effect of poly(ethylene glycol)-phospholipids (PE-PEG) on the interaction of avidin with PEGylated liposomes containing surface-bound biotin ligands. The influence of grafting density and lipopolymer chain length is examined. A simple fluorescence assay involving a receptor-mediated fluorescence increase of BODIPY-labeled avidin upon binding to biotinylated lipids is employed to study the screening effect of submicellar concentrations of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine-N-[poly(ethylene glycol)-2000] (PE-PEG2000) and 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine-N-[poly(ethylene glycol)-5000] (PE-PEG5000) incorporated into 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) liposomes. The results show that incorporation of lipopolymers into DPPC lipid bilayers reduces binding of avidin to the biotinylated liposomes, and it is found that the screening effect of PE-PEG5000 is stronger than that for PE-PEG2000. Thus, the results reveal that both the grafting density and the polymer length of the PE-PEG lipopolymers are of importance for the ability of water-soluble macromolecules to reach the surface of PEG liposomes. Furthermore, it is found that none of the lipopolymers completely prevents avidin from reaching the surface-bound biotin ligands.
KW - Avidin
KW - Biotin
KW - Drug targeting
KW - Lipopolymer
KW - PEG liposomes
KW - Receptor-ligand interaction
KW - Steric stabilization
UR - http://www.scopus.com/inward/record.url?scp=0035910885&partnerID=8YFLogxK
U2 - 10.1016/S0378-5173(00)00633-5
DO - 10.1016/S0378-5173(00)00633-5
M3 - Journal article
C2 - 11282238
AN - SCOPUS:0035910885
VL - 214
SP - 63
EP - 65
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
SN - 0378-5173
IS - 1-2
ER -
ID: 230987518