Screening effect of PEG on avidin binding to liposome surface receptors

Research output: Contribution to journalJournal articleResearchpeer-review

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Screening effect of PEG on avidin binding to liposome surface receptors. / Kaasgaard, Thomas; Mouritsen, Ole G.; Jørgensen, Kent.

In: International Journal of Pharmaceutics, Vol. 214, No. 1-2, 19.02.2001, p. 63-65.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Kaasgaard, T, Mouritsen, OG & Jørgensen, K 2001, 'Screening effect of PEG on avidin binding to liposome surface receptors', International Journal of Pharmaceutics, vol. 214, no. 1-2, pp. 63-65. https://doi.org/10.1016/S0378-5173(00)00633-5

APA

Kaasgaard, T., Mouritsen, O. G., & Jørgensen, K. (2001). Screening effect of PEG on avidin binding to liposome surface receptors. International Journal of Pharmaceutics, 214(1-2), 63-65. https://doi.org/10.1016/S0378-5173(00)00633-5

Vancouver

Kaasgaard T, Mouritsen OG, Jørgensen K. Screening effect of PEG on avidin binding to liposome surface receptors. International Journal of Pharmaceutics. 2001 Feb 19;214(1-2):63-65. https://doi.org/10.1016/S0378-5173(00)00633-5

Author

Kaasgaard, Thomas ; Mouritsen, Ole G. ; Jørgensen, Kent. / Screening effect of PEG on avidin binding to liposome surface receptors. In: International Journal of Pharmaceutics. 2001 ; Vol. 214, No. 1-2. pp. 63-65.

Bibtex

@article{d341a730e27c42eda44e4c4599924edd,
title = "Screening effect of PEG on avidin binding to liposome surface receptors",
abstract = "This study investigates the screening effect of poly(ethylene glycol)-phospholipids (PE-PEG) on the interaction of avidin with PEGylated liposomes containing surface-bound biotin ligands. The influence of grafting density and lipopolymer chain length is examined. A simple fluorescence assay involving a receptor-mediated fluorescence increase of BODIPY-labeled avidin upon binding to biotinylated lipids is employed to study the screening effect of submicellar concentrations of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine-N-[poly(ethylene glycol)-2000] (PE-PEG2000) and 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine-N-[poly(ethylene glycol)-5000] (PE-PEG5000) incorporated into 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) liposomes. The results show that incorporation of lipopolymers into DPPC lipid bilayers reduces binding of avidin to the biotinylated liposomes, and it is found that the screening effect of PE-PEG5000 is stronger than that for PE-PEG2000. Thus, the results reveal that both the grafting density and the polymer length of the PE-PEG lipopolymers are of importance for the ability of water-soluble macromolecules to reach the surface of PEG liposomes. Furthermore, it is found that none of the lipopolymers completely prevents avidin from reaching the surface-bound biotin ligands.",
keywords = "Avidin, Biotin, Drug targeting, Lipopolymer, PEG liposomes, Receptor-ligand interaction, Steric stabilization",
author = "Thomas Kaasgaard and Mouritsen, {Ole G.} and Kent J{\o}rgensen",
year = "2001",
month = "2",
day = "19",
doi = "10.1016/S0378-5173(00)00633-5",
language = "English",
volume = "214",
pages = "63--65",
journal = "International Journal of Pharmaceutics",
issn = "0378-5173",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Screening effect of PEG on avidin binding to liposome surface receptors

AU - Kaasgaard, Thomas

AU - Mouritsen, Ole G.

AU - Jørgensen, Kent

PY - 2001/2/19

Y1 - 2001/2/19

N2 - This study investigates the screening effect of poly(ethylene glycol)-phospholipids (PE-PEG) on the interaction of avidin with PEGylated liposomes containing surface-bound biotin ligands. The influence of grafting density and lipopolymer chain length is examined. A simple fluorescence assay involving a receptor-mediated fluorescence increase of BODIPY-labeled avidin upon binding to biotinylated lipids is employed to study the screening effect of submicellar concentrations of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine-N-[poly(ethylene glycol)-2000] (PE-PEG2000) and 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine-N-[poly(ethylene glycol)-5000] (PE-PEG5000) incorporated into 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) liposomes. The results show that incorporation of lipopolymers into DPPC lipid bilayers reduces binding of avidin to the biotinylated liposomes, and it is found that the screening effect of PE-PEG5000 is stronger than that for PE-PEG2000. Thus, the results reveal that both the grafting density and the polymer length of the PE-PEG lipopolymers are of importance for the ability of water-soluble macromolecules to reach the surface of PEG liposomes. Furthermore, it is found that none of the lipopolymers completely prevents avidin from reaching the surface-bound biotin ligands.

AB - This study investigates the screening effect of poly(ethylene glycol)-phospholipids (PE-PEG) on the interaction of avidin with PEGylated liposomes containing surface-bound biotin ligands. The influence of grafting density and lipopolymer chain length is examined. A simple fluorescence assay involving a receptor-mediated fluorescence increase of BODIPY-labeled avidin upon binding to biotinylated lipids is employed to study the screening effect of submicellar concentrations of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine-N-[poly(ethylene glycol)-2000] (PE-PEG2000) and 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine-N-[poly(ethylene glycol)-5000] (PE-PEG5000) incorporated into 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) liposomes. The results show that incorporation of lipopolymers into DPPC lipid bilayers reduces binding of avidin to the biotinylated liposomes, and it is found that the screening effect of PE-PEG5000 is stronger than that for PE-PEG2000. Thus, the results reveal that both the grafting density and the polymer length of the PE-PEG lipopolymers are of importance for the ability of water-soluble macromolecules to reach the surface of PEG liposomes. Furthermore, it is found that none of the lipopolymers completely prevents avidin from reaching the surface-bound biotin ligands.

KW - Avidin

KW - Biotin

KW - Drug targeting

KW - Lipopolymer

KW - PEG liposomes

KW - Receptor-ligand interaction

KW - Steric stabilization

UR - http://www.scopus.com/inward/record.url?scp=0035910885&partnerID=8YFLogxK

U2 - 10.1016/S0378-5173(00)00633-5

DO - 10.1016/S0378-5173(00)00633-5

M3 - Journal article

C2 - 11282238

AN - SCOPUS:0035910885

VL - 214

SP - 63

EP - 65

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

IS - 1-2

ER -

ID: 230987518