Proteome analysis of Aspergillus niger: lactate added in starch-containing medium can increase production of themycotoxin fumonisin B2 by modifying acetyl-CoA metabolism

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Proteome analysis of Aspergillus niger : lactate added in starch-containing medium can increase production of themycotoxin fumonisin B2 by modifying acetyl-CoA metabolism. / Sørensen, Louise Marie; Lametsch, Rene; Andersen, Mikael R.; Nielsen, Per V.; Frisvad, Jens Christian.

In: BMC Microbiology, Vol. 9, No. 255, 2009.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Sørensen, LM, Lametsch, R, Andersen, MR, Nielsen, PV & Frisvad, JC 2009, 'Proteome analysis of Aspergillus niger: lactate added in starch-containing medium can increase production of themycotoxin fumonisin B2 by modifying acetyl-CoA metabolism', BMC Microbiology, vol. 9, no. 255. https://doi.org/10.1186/1471-2180-9-255

APA

Sørensen, L. M., Lametsch, R., Andersen, M. R., Nielsen, P. V., & Frisvad, J. C. (2009). Proteome analysis of Aspergillus niger: lactate added in starch-containing medium can increase production of themycotoxin fumonisin B2 by modifying acetyl-CoA metabolism. BMC Microbiology, 9(255). https://doi.org/10.1186/1471-2180-9-255

Vancouver

Sørensen LM, Lametsch R, Andersen MR, Nielsen PV, Frisvad JC. Proteome analysis of Aspergillus niger: lactate added in starch-containing medium can increase production of themycotoxin fumonisin B2 by modifying acetyl-CoA metabolism. BMC Microbiology. 2009;9(255). https://doi.org/10.1186/1471-2180-9-255

Author

Sørensen, Louise Marie ; Lametsch, Rene ; Andersen, Mikael R. ; Nielsen, Per V. ; Frisvad, Jens Christian. / Proteome analysis of Aspergillus niger : lactate added in starch-containing medium can increase production of themycotoxin fumonisin B2 by modifying acetyl-CoA metabolism. In: BMC Microbiology. 2009 ; Vol. 9, No. 255.

Bibtex

@article{0c273db02dd011df8ed1000ea68e967b,
title = "Proteome analysis of Aspergillus niger: lactate added in starch-containing medium can increase production of themycotoxin fumonisin B2 by modifying acetyl-CoA metabolism",
abstract = "Background: Aspergillus niger is a filamentous fungus found in the environment, on foods andfeeds and is used as host for production of organic acids, enzymes and proteins. The mycotoxin fumonisin B2 was recently found to be produced by A. niger and hence very little is known about production and regulation of this metabolite. Proteome analysis was used with the purpose to reveal how fumonisin B2 production by A. niger is influenced by starch and lactate in the medium.Results: Fumonisin B2 production by A. niger was significantly increased when lactate and starchwere combined in the medium. Production of a few other A. niger secondary metabolites was affected similarly by lactate and starch (fumonisin B4, orlandin, desmethylkotanin and pyranonigrin A), while production of others was not (ochratoxin A, ochratoxin alpha, malformin A, malformin C, kotanin, aurasperone B and tensidol B). The proteome of A. niger was clearly different during growth on media containing 3{\%} starch, 3{\%} starch + 3{\%} lactate or 3{\%} lactate. The identity of 59 spots was obtained, mainly those showing higher or lower expression levels on medium with starch and lactate. Many of them were enzymes in primary metabolism and other processes that affect the intracellular level of acetyl-CoA or NADPH. This included enzymes in the pentose phosphate pathway, pyruvate metabolism, the tricarboxylic acid cycle, ammonium assimilation, fatty acid biosynthesis and oxidative stress protection.Conclusions: Lactate added in a medium containing nitrate and starch can increase fumonisin B2 production by A. niger as well as production of some other secondary metabolites. Changes in the balance of intracellular metabolites towards a higher level of carbon passing through acetyl-CoA and a high capacity to regenerate NADPH during growth on medium with starch and lactate were found to be the likely cause of this effect. The results lead to the hypothesis that fumonisin production by A. niger is regulated by acetyl-CoA.",
author = "S{\o}rensen, {Louise Marie} and Rene Lametsch and Andersen, {Mikael R.} and Nielsen, {Per V.} and Frisvad, {Jens Christian}",
year = "2009",
doi = "10.1186/1471-2180-9-255",
language = "English",
volume = "9",
journal = "B M C Microbiology",
issn = "1471-2180",
publisher = "BioMed Central Ltd.",
number = "255",

}

RIS

TY - JOUR

T1 - Proteome analysis of Aspergillus niger

T2 - lactate added in starch-containing medium can increase production of themycotoxin fumonisin B2 by modifying acetyl-CoA metabolism

AU - Sørensen, Louise Marie

AU - Lametsch, Rene

AU - Andersen, Mikael R.

AU - Nielsen, Per V.

AU - Frisvad, Jens Christian

PY - 2009

Y1 - 2009

N2 - Background: Aspergillus niger is a filamentous fungus found in the environment, on foods andfeeds and is used as host for production of organic acids, enzymes and proteins. The mycotoxin fumonisin B2 was recently found to be produced by A. niger and hence very little is known about production and regulation of this metabolite. Proteome analysis was used with the purpose to reveal how fumonisin B2 production by A. niger is influenced by starch and lactate in the medium.Results: Fumonisin B2 production by A. niger was significantly increased when lactate and starchwere combined in the medium. Production of a few other A. niger secondary metabolites was affected similarly by lactate and starch (fumonisin B4, orlandin, desmethylkotanin and pyranonigrin A), while production of others was not (ochratoxin A, ochratoxin alpha, malformin A, malformin C, kotanin, aurasperone B and tensidol B). The proteome of A. niger was clearly different during growth on media containing 3% starch, 3% starch + 3% lactate or 3% lactate. The identity of 59 spots was obtained, mainly those showing higher or lower expression levels on medium with starch and lactate. Many of them were enzymes in primary metabolism and other processes that affect the intracellular level of acetyl-CoA or NADPH. This included enzymes in the pentose phosphate pathway, pyruvate metabolism, the tricarboxylic acid cycle, ammonium assimilation, fatty acid biosynthesis and oxidative stress protection.Conclusions: Lactate added in a medium containing nitrate and starch can increase fumonisin B2 production by A. niger as well as production of some other secondary metabolites. Changes in the balance of intracellular metabolites towards a higher level of carbon passing through acetyl-CoA and a high capacity to regenerate NADPH during growth on medium with starch and lactate were found to be the likely cause of this effect. The results lead to the hypothesis that fumonisin production by A. niger is regulated by acetyl-CoA.

AB - Background: Aspergillus niger is a filamentous fungus found in the environment, on foods andfeeds and is used as host for production of organic acids, enzymes and proteins. The mycotoxin fumonisin B2 was recently found to be produced by A. niger and hence very little is known about production and regulation of this metabolite. Proteome analysis was used with the purpose to reveal how fumonisin B2 production by A. niger is influenced by starch and lactate in the medium.Results: Fumonisin B2 production by A. niger was significantly increased when lactate and starchwere combined in the medium. Production of a few other A. niger secondary metabolites was affected similarly by lactate and starch (fumonisin B4, orlandin, desmethylkotanin and pyranonigrin A), while production of others was not (ochratoxin A, ochratoxin alpha, malformin A, malformin C, kotanin, aurasperone B and tensidol B). The proteome of A. niger was clearly different during growth on media containing 3% starch, 3% starch + 3% lactate or 3% lactate. The identity of 59 spots was obtained, mainly those showing higher or lower expression levels on medium with starch and lactate. Many of them were enzymes in primary metabolism and other processes that affect the intracellular level of acetyl-CoA or NADPH. This included enzymes in the pentose phosphate pathway, pyruvate metabolism, the tricarboxylic acid cycle, ammonium assimilation, fatty acid biosynthesis and oxidative stress protection.Conclusions: Lactate added in a medium containing nitrate and starch can increase fumonisin B2 production by A. niger as well as production of some other secondary metabolites. Changes in the balance of intracellular metabolites towards a higher level of carbon passing through acetyl-CoA and a high capacity to regenerate NADPH during growth on medium with starch and lactate were found to be the likely cause of this effect. The results lead to the hypothesis that fumonisin production by A. niger is regulated by acetyl-CoA.

U2 - 10.1186/1471-2180-9-255

DO - 10.1186/1471-2180-9-255

M3 - Journal article

VL - 9

JO - B M C Microbiology

JF - B M C Microbiology

SN - 1471-2180

IS - 255

ER -

ID: 18584689