Phytase-producing capacity of yeasts isolated from traditional African fermented food products and PHYPk gene expression of Pichia kudriavzevii strains

Research output: Contribution to journalJournal articleResearchpeer-review

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Phytase-producing capacity of yeasts isolated from traditional African fermented food products and PHYPk gene expression of Pichia kudriavzevii strains. / Greppi, Anna; Krych, Lukasz; Costantini, Antonella; Rantsiou, Kalliopi; Hounhouigan, D. Joseph; Arneborg, Nils; Cocolin, Luca; Jespersen, Lene.

In: International Journal of Food Microbiology, Vol. 205, 2015, p. 81-89.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Greppi, A, Krych, L, Costantini, A, Rantsiou, K, Hounhouigan, DJ, Arneborg, N, Cocolin, L & Jespersen, L 2015, 'Phytase-producing capacity of yeasts isolated from traditional African fermented food products and PHYPk gene expression of Pichia kudriavzevii strains', International Journal of Food Microbiology, vol. 205, pp. 81-89. https://doi.org/10.1016/j.ijfoodmicro.2015.04.011

APA

Greppi, A., Krych, L., Costantini, A., Rantsiou, K., Hounhouigan, D. J., Arneborg, N., ... Jespersen, L. (2015). Phytase-producing capacity of yeasts isolated from traditional African fermented food products and PHYPk gene expression of Pichia kudriavzevii strains. International Journal of Food Microbiology, 205, 81-89. https://doi.org/10.1016/j.ijfoodmicro.2015.04.011

Vancouver

Greppi A, Krych L, Costantini A, Rantsiou K, Hounhouigan DJ, Arneborg N et al. Phytase-producing capacity of yeasts isolated from traditional African fermented food products and PHYPk gene expression of Pichia kudriavzevii strains. International Journal of Food Microbiology. 2015;205:81-89. https://doi.org/10.1016/j.ijfoodmicro.2015.04.011

Author

Greppi, Anna ; Krych, Lukasz ; Costantini, Antonella ; Rantsiou, Kalliopi ; Hounhouigan, D. Joseph ; Arneborg, Nils ; Cocolin, Luca ; Jespersen, Lene. / Phytase-producing capacity of yeasts isolated from traditional African fermented food products and PHYPk gene expression of Pichia kudriavzevii strains. In: International Journal of Food Microbiology. 2015 ; Vol. 205. pp. 81-89.

Bibtex

@article{9e914c99ef084ee1ab5d532ea49209f0,
title = "Phytase-producing capacity of yeasts isolated from traditional African fermented food products and PHYPk gene expression of Pichia kudriavzevii strains",
abstract = "Phytate is known as a strong chelate of minerals causing their reduced uptake by the human intestine. Ninety-three yeast isolates from traditional African fermented food products, belonging to nine species (Pichia kudriavzevii, Saccharomyces cerevisiae, Clavispora lusitaniae, Kluyveromyces marxianus, Millerozyma farinosa, Candida glabrata, Wickerhamomyces anomalus, Hanseniaspora guilliermondii and Debaryomyces nepalensis) were screened for phytase production on solid and liquid media. 95{\%} were able to grow in the presence of phytate as sole phosphate source, P. kudriavzevii being the best growing species. A phytase coding gene of P. kudriavzevii (PHYPk) was identified and its expression was studied during growth by RT-qPCR. The expression level of PHYPk was significantly higher in phytate-medium, compared to phosphate-medium. In phytate-medium expression was seen in the lag phase. Significant differences in gene expression were detected among the strains as well as between the media. A correlation was found between the PHYPk expression and phytase extracellular activity.",
author = "Anna Greppi and Lukasz Krych and Antonella Costantini and Kalliopi Rantsiou and Hounhouigan, {D. Joseph} and Nils Arneborg and Luca Cocolin and Lene Jespersen",
note = "Copyright {\circledC} 2015 Elsevier B.V. All rights reserved.",
year = "2015",
doi = "10.1016/j.ijfoodmicro.2015.04.011",
language = "English",
volume = "205",
pages = "81--89",
journal = "International Journal of Food Microbiology",
issn = "0168-1605",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Phytase-producing capacity of yeasts isolated from traditional African fermented food products and PHYPk gene expression of Pichia kudriavzevii strains

AU - Greppi, Anna

AU - Krych, Lukasz

AU - Costantini, Antonella

AU - Rantsiou, Kalliopi

AU - Hounhouigan, D. Joseph

AU - Arneborg, Nils

AU - Cocolin, Luca

AU - Jespersen, Lene

N1 - Copyright © 2015 Elsevier B.V. All rights reserved.

PY - 2015

Y1 - 2015

N2 - Phytate is known as a strong chelate of minerals causing their reduced uptake by the human intestine. Ninety-three yeast isolates from traditional African fermented food products, belonging to nine species (Pichia kudriavzevii, Saccharomyces cerevisiae, Clavispora lusitaniae, Kluyveromyces marxianus, Millerozyma farinosa, Candida glabrata, Wickerhamomyces anomalus, Hanseniaspora guilliermondii and Debaryomyces nepalensis) were screened for phytase production on solid and liquid media. 95% were able to grow in the presence of phytate as sole phosphate source, P. kudriavzevii being the best growing species. A phytase coding gene of P. kudriavzevii (PHYPk) was identified and its expression was studied during growth by RT-qPCR. The expression level of PHYPk was significantly higher in phytate-medium, compared to phosphate-medium. In phytate-medium expression was seen in the lag phase. Significant differences in gene expression were detected among the strains as well as between the media. A correlation was found between the PHYPk expression and phytase extracellular activity.

AB - Phytate is known as a strong chelate of minerals causing their reduced uptake by the human intestine. Ninety-three yeast isolates from traditional African fermented food products, belonging to nine species (Pichia kudriavzevii, Saccharomyces cerevisiae, Clavispora lusitaniae, Kluyveromyces marxianus, Millerozyma farinosa, Candida glabrata, Wickerhamomyces anomalus, Hanseniaspora guilliermondii and Debaryomyces nepalensis) were screened for phytase production on solid and liquid media. 95% were able to grow in the presence of phytate as sole phosphate source, P. kudriavzevii being the best growing species. A phytase coding gene of P. kudriavzevii (PHYPk) was identified and its expression was studied during growth by RT-qPCR. The expression level of PHYPk was significantly higher in phytate-medium, compared to phosphate-medium. In phytate-medium expression was seen in the lag phase. Significant differences in gene expression were detected among the strains as well as between the media. A correlation was found between the PHYPk expression and phytase extracellular activity.

U2 - 10.1016/j.ijfoodmicro.2015.04.011

DO - 10.1016/j.ijfoodmicro.2015.04.011

M3 - Journal article

C2 - 25910031

VL - 205

SP - 81

EP - 89

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

SN - 0168-1605

ER -

ID: 140162303