TY - JOUR

T1 - Perspective on the Effect of Protein Extraction Method on the Antinutritional Factor (ANF) Content in Seeds

AU - Amin, Ashwitha

AU - Petersen, Iben Lykke

AU - Malmberg, Christian

AU - Orlien, Vibeke

N1 - Publisher Copyright: © 2022 by the authors.

PY - 2022

Y1 - 2022

N2 - Plants contain antinutritional factors (ANFs) with negative impact on protein digestibility and nutritional quality. We aim to highlight the importance of extraction method on the coextraction of different ANFs to put focus on this challenge regarding the usability of plant-protein ingredients. We mainly focus on the research behind the established, industrial techniques, wet extraction and dry fractionation, in relation to ANFs. These methods give protein powders differing considerably in protein concentrations and ANF contents. Wet extraction offers the highest protein concentration (typically >85%) and lowest ANF content (reduction between 75% and 96%, depending on the type of ANF). Usage of chemicals and energy has pushed the development of new extraction techniques; hence dry fractionation is explored. However, it was typically found that dry fractionation resulted in an accumulation of some ANFs in the resultant protein concentrates. Innovative separation methods, like selective electrodialysis, forward osmosis, immobilized metal affinity, and zinc reductants, are suggested to be effective processes for concentration, purification, and selection of proteins. In general, more research is needed, and strategies to sophisticate extraction methods, for example by combining with pre- and postprocessing, and ensure high quality of the protein fractions are mandatory.

AB - Plants contain antinutritional factors (ANFs) with negative impact on protein digestibility and nutritional quality. We aim to highlight the importance of extraction method on the coextraction of different ANFs to put focus on this challenge regarding the usability of plant-protein ingredients. We mainly focus on the research behind the established, industrial techniques, wet extraction and dry fractionation, in relation to ANFs. These methods give protein powders differing considerably in protein concentrations and ANF contents. Wet extraction offers the highest protein concentration (typically >85%) and lowest ANF content (reduction between 75% and 96%, depending on the type of ANF). Usage of chemicals and energy has pushed the development of new extraction techniques; hence dry fractionation is explored. However, it was typically found that dry fractionation resulted in an accumulation of some ANFs in the resultant protein concentrates. Innovative separation methods, like selective electrodialysis, forward osmosis, immobilized metal affinity, and zinc reductants, are suggested to be effective processes for concentration, purification, and selection of proteins. In general, more research is needed, and strategies to sophisticate extraction methods, for example by combining with pre- and postprocessing, and ensure high quality of the protein fractions are mandatory.

KW - antinutritional factor (ANF)

KW - cereal

KW - dry fractionation

KW - legume

KW - oilseed

KW - protein extraction

KW - wet extraction

U2 - 10.1021/acsfoodscitech.1c00464

DO - 10.1021/acsfoodscitech.1c00464

M3 - Review

AN - SCOPUS:85127892990

VL - 2

SP - 604

EP - 612

JO - ACS Food Science & Technology

JF - ACS Food Science & Technology

SN - 2692-1944

IS - 4

ER -