PCR amplification of repetitive sequences as a possible approach in relative species quantification
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PCR amplification of repetitive sequences as a possible approach in relative species quantification. / Ballin, Nicolai Zederkopff; Vogensen, Finn Kvist; Karlsson, Anders H.
In: Meat Science, Vol. 90, No. 2, 2012, p. 438-443.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - PCR amplification of repetitive sequences as a possible approach in relative species quantification
AU - Ballin, Nicolai Zederkopff
AU - Vogensen, Finn Kvist
AU - Karlsson, Anders H
PY - 2012
Y1 - 2012
N2 - Abstract Both relative and absolute quantifications are possible in species quantification when single copy genomic DNA is used. However, amplification of single copy genomic DNA does not allow a limit of detection as low as one obtained from amplification of repetitive sequences. Amplification of repetitive sequences is therefore frequently used in absolute quantification but problems occur in relative quantification as the number of repetitive sequences is unknown. A promising approach was developed where data from amplification of repetitive sequences were used in relative quantification of species in binary mixtures. PCR LUX primers were designed that amplify repetitive and single copy sequences to establish the species dependent number (constants) (SDC) of amplified repetitive sequences per genome. The SDCs and data from amplification of repetitive sequences were tested for their applicability to relatively quantify the amount of chicken DNA in a binary mixture of chicken DNA and pig DNA. However, the designed PCR primers lack the specificity required for regulatory species control.
AB - Abstract Both relative and absolute quantifications are possible in species quantification when single copy genomic DNA is used. However, amplification of single copy genomic DNA does not allow a limit of detection as low as one obtained from amplification of repetitive sequences. Amplification of repetitive sequences is therefore frequently used in absolute quantification but problems occur in relative quantification as the number of repetitive sequences is unknown. A promising approach was developed where data from amplification of repetitive sequences were used in relative quantification of species in binary mixtures. PCR LUX primers were designed that amplify repetitive and single copy sequences to establish the species dependent number (constants) (SDC) of amplified repetitive sequences per genome. The SDCs and data from amplification of repetitive sequences were tested for their applicability to relatively quantify the amount of chicken DNA in a binary mixture of chicken DNA and pig DNA. However, the designed PCR primers lack the specificity required for regulatory species control.
U2 - 10.1016/j.meatsci.2011.09.002
DO - 10.1016/j.meatsci.2011.09.002
M3 - Journal article
C2 - 21944936
VL - 90
SP - 438
EP - 443
JO - Meat Science
JF - Meat Science
SN - 0309-1740
IS - 2
ER -
ID: 49644889