Pandemic influenza 1918 H1N1 and 1968 H3N2 DNA vaccines induce cross-reactive immunity in ferrets against infection with viruses drifted for decades

Research output: Contribution to journalJournal articlepeer-review

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Pandemic influenza 1918 H1N1 and 1968 H3N2 DNA vaccines induce cross-reactive immunity in ferrets against infection with viruses drifted for decades. / Bragstad, Karoline; Martel, Cyril; Thomsen, Joakim S.; Jensen, Kim Lynge; Nielsen, Lars P.; Aasted, Bent; Fomsgaard, Anders.

In: Influenza and Other Respiratory Viruses, Vol. 5, No. 1, 2011, p. 13-23.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Bragstad, K, Martel, C, Thomsen, JS, Jensen, KL, Nielsen, LP, Aasted, B & Fomsgaard, A 2011, 'Pandemic influenza 1918 H1N1 and 1968 H3N2 DNA vaccines induce cross-reactive immunity in ferrets against infection with viruses drifted for decades', Influenza and Other Respiratory Viruses, vol. 5, no. 1, pp. 13-23. https://doi.org/10.1111/j.1750-2659.2010.00177.x

APA

Bragstad, K., Martel, C., Thomsen, J. S., Jensen, K. L., Nielsen, L. P., Aasted, B., & Fomsgaard, A. (2011). Pandemic influenza 1918 H1N1 and 1968 H3N2 DNA vaccines induce cross-reactive immunity in ferrets against infection with viruses drifted for decades. Influenza and Other Respiratory Viruses, 5(1), 13-23. https://doi.org/10.1111/j.1750-2659.2010.00177.x

Vancouver

Bragstad K, Martel C, Thomsen JS, Jensen KL, Nielsen LP, Aasted B et al. Pandemic influenza 1918 H1N1 and 1968 H3N2 DNA vaccines induce cross-reactive immunity in ferrets against infection with viruses drifted for decades. Influenza and Other Respiratory Viruses. 2011;5(1):13-23. https://doi.org/10.1111/j.1750-2659.2010.00177.x

Author

Bragstad, Karoline ; Martel, Cyril ; Thomsen, Joakim S. ; Jensen, Kim Lynge ; Nielsen, Lars P. ; Aasted, Bent ; Fomsgaard, Anders. / Pandemic influenza 1918 H1N1 and 1968 H3N2 DNA vaccines induce cross-reactive immunity in ferrets against infection with viruses drifted for decades. In: Influenza and Other Respiratory Viruses. 2011 ; Vol. 5, No. 1. pp. 13-23.

Bibtex

@article{d2e1d38cac004b9fbc0b594712b83e31,
title = "Pandemic influenza 1918 H1N1 and 1968 H3N2 DNA vaccines induce cross-reactive immunity in ferrets against infection with viruses drifted for decades",
abstract = "Please cite this paper as: Bragstad et al. (2010) Pandemic influenza 1918 H1N1 and 1968 H3N2 DNA vaccines induce cross-reactive immunity in ferrets against infection with viruses drifted for decades. Influenza and Other Respiratory Viruses 5(1), 13-23. Background Alternative influenza vaccines and vaccine production forms are needed as the conventional protein vaccines do not induce broad cross-reactivity against drifted strains. Furthermore, fast vaccine production is especially important in a pandemic situation, and broader vaccine reactivity would diminish the need for frequent change in the vaccine formulations. Objective In this study, we compared the ability of pandemic influenza DNA vaccines to induce immunity against distantly related strains within a subtype with the immunity induced by conventional trivalent protein vaccines against homologous virus challenge. Methods Ferrets were immunised by particle-mediated epidermal delivery (gene gun) with DNA vaccines based on the haemagglutinin (HA) and neuraminidase (NA) and/or the matrix (M) and nucleoprotein genes of the 1918 H1N1 Spanish influenza pandemic virus or the 1968 H3N2 Hong Kong influenza pandemic virus. The animals were challenged with contemporary H1N1 or H3N2 viruses. Results We demonstrated that DNA vaccines encoding proteins of the original 1918 H1N1 pandemic virus induced protective cross-reactive immune responses in ferrets against infection with a 1947 H1N1 virus and a recent 1999 H1N1 virus. Similarly, a DNA vaccine, based on the HA and NA of the 1968 H3N2 pandemic virus, induced cross-reactive immune responses against a recent 2005 H3N2 virus challenge. Conclusions DNA vaccines based on pandemic or recent seasonal influenza genes induced cross-reactive immunity against contemporary virus challenge as good as or superior to contemporary conventional trivalent protein vaccines. This suggests a unique ability of influenza DNA to induce cross-protective immunity against both contemporary and long-time drifted viruses.",
keywords = "Former LIFE faculty, Cross-reactive immunity, DNA vaccine, influenza, pandemic influenza",
author = "Karoline Bragstad and Cyril Martel and Thomsen, {Joakim S.} and Jensen, {Kim Lynge} and Nielsen, {Lars P.} and Bent Aasted and Anders Fomsgaard",
note = "{\textcopyright} 2010 Blackwell Publishing Ltd.",
year = "2011",
doi = "10.1111/j.1750-2659.2010.00177.x",
language = "English",
volume = "5",
pages = "13--23",
journal = "Influenza and other Respiratory Viruses",
issn = "1750-2640",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Pandemic influenza 1918 H1N1 and 1968 H3N2 DNA vaccines induce cross-reactive immunity in ferrets against infection with viruses drifted for decades

AU - Bragstad, Karoline

AU - Martel, Cyril

AU - Thomsen, Joakim S.

AU - Jensen, Kim Lynge

AU - Nielsen, Lars P.

AU - Aasted, Bent

AU - Fomsgaard, Anders

N1 - © 2010 Blackwell Publishing Ltd.

PY - 2011

Y1 - 2011

N2 - Please cite this paper as: Bragstad et al. (2010) Pandemic influenza 1918 H1N1 and 1968 H3N2 DNA vaccines induce cross-reactive immunity in ferrets against infection with viruses drifted for decades. Influenza and Other Respiratory Viruses 5(1), 13-23. Background Alternative influenza vaccines and vaccine production forms are needed as the conventional protein vaccines do not induce broad cross-reactivity against drifted strains. Furthermore, fast vaccine production is especially important in a pandemic situation, and broader vaccine reactivity would diminish the need for frequent change in the vaccine formulations. Objective In this study, we compared the ability of pandemic influenza DNA vaccines to induce immunity against distantly related strains within a subtype with the immunity induced by conventional trivalent protein vaccines against homologous virus challenge. Methods Ferrets were immunised by particle-mediated epidermal delivery (gene gun) with DNA vaccines based on the haemagglutinin (HA) and neuraminidase (NA) and/or the matrix (M) and nucleoprotein genes of the 1918 H1N1 Spanish influenza pandemic virus or the 1968 H3N2 Hong Kong influenza pandemic virus. The animals were challenged with contemporary H1N1 or H3N2 viruses. Results We demonstrated that DNA vaccines encoding proteins of the original 1918 H1N1 pandemic virus induced protective cross-reactive immune responses in ferrets against infection with a 1947 H1N1 virus and a recent 1999 H1N1 virus. Similarly, a DNA vaccine, based on the HA and NA of the 1968 H3N2 pandemic virus, induced cross-reactive immune responses against a recent 2005 H3N2 virus challenge. Conclusions DNA vaccines based on pandemic or recent seasonal influenza genes induced cross-reactive immunity against contemporary virus challenge as good as or superior to contemporary conventional trivalent protein vaccines. This suggests a unique ability of influenza DNA to induce cross-protective immunity against both contemporary and long-time drifted viruses.

AB - Please cite this paper as: Bragstad et al. (2010) Pandemic influenza 1918 H1N1 and 1968 H3N2 DNA vaccines induce cross-reactive immunity in ferrets against infection with viruses drifted for decades. Influenza and Other Respiratory Viruses 5(1), 13-23. Background Alternative influenza vaccines and vaccine production forms are needed as the conventional protein vaccines do not induce broad cross-reactivity against drifted strains. Furthermore, fast vaccine production is especially important in a pandemic situation, and broader vaccine reactivity would diminish the need for frequent change in the vaccine formulations. Objective In this study, we compared the ability of pandemic influenza DNA vaccines to induce immunity against distantly related strains within a subtype with the immunity induced by conventional trivalent protein vaccines against homologous virus challenge. Methods Ferrets were immunised by particle-mediated epidermal delivery (gene gun) with DNA vaccines based on the haemagglutinin (HA) and neuraminidase (NA) and/or the matrix (M) and nucleoprotein genes of the 1918 H1N1 Spanish influenza pandemic virus or the 1968 H3N2 Hong Kong influenza pandemic virus. The animals were challenged with contemporary H1N1 or H3N2 viruses. Results We demonstrated that DNA vaccines encoding proteins of the original 1918 H1N1 pandemic virus induced protective cross-reactive immune responses in ferrets against infection with a 1947 H1N1 virus and a recent 1999 H1N1 virus. Similarly, a DNA vaccine, based on the HA and NA of the 1968 H3N2 pandemic virus, induced cross-reactive immune responses against a recent 2005 H3N2 virus challenge. Conclusions DNA vaccines based on pandemic or recent seasonal influenza genes induced cross-reactive immunity against contemporary virus challenge as good as or superior to contemporary conventional trivalent protein vaccines. This suggests a unique ability of influenza DNA to induce cross-protective immunity against both contemporary and long-time drifted viruses.

KW - Former LIFE faculty

KW - Cross-reactive immunity

KW - DNA vaccine

KW - influenza

KW - pandemic influenza

U2 - 10.1111/j.1750-2659.2010.00177.x

DO - 10.1111/j.1750-2659.2010.00177.x

M3 - Journal article

C2 - 21138536

VL - 5

SP - 13

EP - 23

JO - Influenza and other Respiratory Viruses

JF - Influenza and other Respiratory Viruses

SN - 1750-2640

IS - 1

ER -

ID: 32168706