Oral LPS Dosing Induces Local Immunological Changes in the Pancreatic Lymph Nodes in Mice

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Oral LPS Dosing Induces Local Immunological Changes in the Pancreatic Lymph Nodes in Mice. / Kihl, Pernille; Krych, Lukasz; Deng, Ling; Kildemoes, Anna Overgaard; Laigaard, Ann; Hansen, Lars Hestbjerg; Hansen, Camilla Hartmann Friis; Buschard, Karsten; Nielsen, Dennis Sandris; Hansen, Axel Kornerup.

In: Journal of Diabetes Research, Vol. 2019, 1649279, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Kihl, P, Krych, L, Deng, L, Kildemoes, AO, Laigaard, A, Hansen, LH, Hansen, CHF, Buschard, K, Nielsen, DS & Hansen, AK 2019, 'Oral LPS Dosing Induces Local Immunological Changes in the Pancreatic Lymph Nodes in Mice', Journal of Diabetes Research, vol. 2019, 1649279. https://doi.org/10.1155/2019/1649279

APA

Kihl, P., Krych, L., Deng, L., Kildemoes, A. O., Laigaard, A., Hansen, L. H., ... Hansen, A. K. (2019). Oral LPS Dosing Induces Local Immunological Changes in the Pancreatic Lymph Nodes in Mice. Journal of Diabetes Research, 2019, [1649279]. https://doi.org/10.1155/2019/1649279

Vancouver

Kihl P, Krych L, Deng L, Kildemoes AO, Laigaard A, Hansen LH et al. Oral LPS Dosing Induces Local Immunological Changes in the Pancreatic Lymph Nodes in Mice. Journal of Diabetes Research. 2019;2019. 1649279. https://doi.org/10.1155/2019/1649279

Author

Kihl, Pernille ; Krych, Lukasz ; Deng, Ling ; Kildemoes, Anna Overgaard ; Laigaard, Ann ; Hansen, Lars Hestbjerg ; Hansen, Camilla Hartmann Friis ; Buschard, Karsten ; Nielsen, Dennis Sandris ; Hansen, Axel Kornerup. / Oral LPS Dosing Induces Local Immunological Changes in the Pancreatic Lymph Nodes in Mice. In: Journal of Diabetes Research. 2019 ; Vol. 2019.

Bibtex

@article{9dc3739f25a5422780e9eb876c55e677,
title = "Oral LPS Dosing Induces Local Immunological Changes in the Pancreatic Lymph Nodes in Mice",
abstract = "Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D). Previously, a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria was observed, when vancomycin was given to pups of nonobese diabetic (NOD) mice. While the T1D incidence reducing effect of A. muciniphila has been shown in further studies, little is known as to whether the increased abundance of LPS-carrying bacteria also has a protective effect. Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected. However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion. It is, therefore, concluded that early life oral E. coli LPS has an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life.",
author = "Pernille Kihl and Lukasz Krych and Ling Deng and Kildemoes, {Anna Overgaard} and Ann Laigaard and Hansen, {Lars Hestbjerg} and Hansen, {Camilla Hartmann Friis} and Karsten Buschard and Nielsen, {Dennis Sandris} and Hansen, {Axel Kornerup}",
year = "2019",
doi = "10.1155/2019/1649279",
language = "English",
volume = "2019",
journal = "Journal of Diabetes Research",
issn = "2314-6745",
publisher = "Hindawi Publishing Corporation",

}

RIS

TY - JOUR

T1 - Oral LPS Dosing Induces Local Immunological Changes in the Pancreatic Lymph Nodes in Mice

AU - Kihl, Pernille

AU - Krych, Lukasz

AU - Deng, Ling

AU - Kildemoes, Anna Overgaard

AU - Laigaard, Ann

AU - Hansen, Lars Hestbjerg

AU - Hansen, Camilla Hartmann Friis

AU - Buschard, Karsten

AU - Nielsen, Dennis Sandris

AU - Hansen, Axel Kornerup

PY - 2019

Y1 - 2019

N2 - Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D). Previously, a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria was observed, when vancomycin was given to pups of nonobese diabetic (NOD) mice. While the T1D incidence reducing effect of A. muciniphila has been shown in further studies, little is known as to whether the increased abundance of LPS-carrying bacteria also has a protective effect. Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected. However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion. It is, therefore, concluded that early life oral E. coli LPS has an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life.

AB - Lacking the initial contact between the immune system and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharides (LPS), early in life, may be regarded as one of the causal factors of the increasing global increase in the incidence of autoimmune diseases, such as type 1 diabetes (T1D). Previously, a reduced incidence of T1D accompanied by dramatically increased abundances of both the mucin-metabolising bacterium Akkermansia muciniphila, and LPS-carrying Proteobacteria was observed, when vancomycin was given to pups of nonobese diabetic (NOD) mice. While the T1D incidence reducing effect of A. muciniphila has been shown in further studies, little is known as to whether the increased abundance of LPS-carrying bacteria also has a protective effect. Therefore, we fed NOD pups with Eschericia coli LPS orally from birth to weaning, which decreased the gene expressions of TNFα, IL-10, IL-6, IFNγ, IL-1β, IL-2, IL-4, and FoxP3 in the pancreatic lymph nodes, while the same gene expression profile in the spleen was unaffected. However, no significant difference in the incidence of T1D, gut microbiota composition, or ileum expression of the genetic markers of gut permeability, Claudin8, Occludin, Zonulin-1 (Tjp1), Claudin15, Muc1, and Muc2 were observed in relation to LPS ingestion. It is, therefore, concluded that early life oral E. coli LPS has an impact on the local immune response, which, however, did not influence T1D incidence in NOD mice later in life.

U2 - 10.1155/2019/1649279

DO - 10.1155/2019/1649279

M3 - Journal article

VL - 2019

JO - Journal of Diabetes Research

JF - Journal of Diabetes Research

SN - 2314-6745

M1 - 1649279

ER -

ID: 215562424