Optimization and modeling of the remote loading of luciferin into liposomes

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Optimization and modeling of the remote loading of luciferin into liposomes. / Hansen, Anders Højgaard; Lomholt, Michael A.; Hansen, Per Lyngs; Mouritsen, Ole G.; Arouri, Ahmad.

In: International Journal of Pharmaceutics, Vol. 508, No. 1-2, 2016, p. 128-134.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Hansen, AH, Lomholt, MA, Hansen, PL, Mouritsen, OG & Arouri, A 2016, 'Optimization and modeling of the remote loading of luciferin into liposomes', International Journal of Pharmaceutics, vol. 508, no. 1-2, pp. 128-134. https://doi.org/10.1016/j.ijpharm.2016.04.055

APA

Hansen, A. H., Lomholt, M. A., Hansen, P. L., Mouritsen, O. G., & Arouri, A. (2016). Optimization and modeling of the remote loading of luciferin into liposomes. International Journal of Pharmaceutics, 508(1-2), 128-134. https://doi.org/10.1016/j.ijpharm.2016.04.055

Vancouver

Hansen AH, Lomholt MA, Hansen PL, Mouritsen OG, Arouri A. Optimization and modeling of the remote loading of luciferin into liposomes. International Journal of Pharmaceutics. 2016;508(1-2):128-134. https://doi.org/10.1016/j.ijpharm.2016.04.055

Author

Hansen, Anders Højgaard ; Lomholt, Michael A. ; Hansen, Per Lyngs ; Mouritsen, Ole G. ; Arouri, Ahmad. / Optimization and modeling of the remote loading of luciferin into liposomes. In: International Journal of Pharmaceutics. 2016 ; Vol. 508, No. 1-2. pp. 128-134.

Bibtex

@article{3c011a47ec1d4e0cb01cc86890d4a288,
title = "Optimization and modeling of the remote loading of luciferin into liposomes",
abstract = "We carried out a mechanistic study to characterize and optimize the remote loading of luciferin into preformed liposomes of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine/1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPC/DPPG) 7:3 mixtures. The influence of the loading agent (acetate, propionate, butyrate), the metal counterion (Na+, K+, Ca+2, Mg+2), and the initial extra-liposomal amount of luciferin (nLadd) on the luciferin Loading Efficiency (LE{\%}) and luciferin-to-lipid weight ratio, i.e., Loading Capacity (LC), in the final formulation was determined. In addition, the effect of the loading process on the colloidal stability and phase behavior of the liposomes was monitored. Based on our experimental results, a theoretical model was developed to describe the course of luciferin remote loading. It was found that the highest luciferin loading was obtained with magnesium acetate. The use of longer aliphatic carboxylates or inorganic proton donors pronouncedly reduced luciferin loading, whereas the effect of the counterion was modest. The remote-loading process barely affected the colloidal stability and drug retention of the liposomes, albeit with moderate luciferin-induced membrane perturbations. The correlation between luciferin loading, expressed as LE{\%} and LC, and nLadd was established, and under our conditions the maximum LC was attained using an nLadd of around 2.6 μmol. Higher amounts of luciferin tend to pronouncedly perturb the liposome stability and luciferin retention. Our theoretical model furnishes a fair quantitative description of the correlation between nLadd and luciferin loading, and a membrane permeability coefficient for uncharged luciferin of 1 × 10-8 cm/s could be determined. We believe that our study will prove very useful to optimize the remote-loading strategies of moderately polar carboxylic acid drugs in general.",
keywords = "Carboxylate gradient, Diffusion, Drug encapsulation, Liposome, Luciferin remote loading, Modeling, Phospholipase A enzyme",
author = "Hansen, {Anders H{\o}jgaard} and Lomholt, {Michael A.} and Hansen, {Per Lyngs} and Mouritsen, {Ole G.} and Ahmad Arouri",
year = "2016",
doi = "10.1016/j.ijpharm.2016.04.055",
language = "English",
volume = "508",
pages = "128--134",
journal = "International Journal of Pharmaceutics",
issn = "0378-5173",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Optimization and modeling of the remote loading of luciferin into liposomes

AU - Hansen, Anders Højgaard

AU - Lomholt, Michael A.

AU - Hansen, Per Lyngs

AU - Mouritsen, Ole G.

AU - Arouri, Ahmad

PY - 2016

Y1 - 2016

N2 - We carried out a mechanistic study to characterize and optimize the remote loading of luciferin into preformed liposomes of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine/1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPC/DPPG) 7:3 mixtures. The influence of the loading agent (acetate, propionate, butyrate), the metal counterion (Na+, K+, Ca+2, Mg+2), and the initial extra-liposomal amount of luciferin (nLadd) on the luciferin Loading Efficiency (LE%) and luciferin-to-lipid weight ratio, i.e., Loading Capacity (LC), in the final formulation was determined. In addition, the effect of the loading process on the colloidal stability and phase behavior of the liposomes was monitored. Based on our experimental results, a theoretical model was developed to describe the course of luciferin remote loading. It was found that the highest luciferin loading was obtained with magnesium acetate. The use of longer aliphatic carboxylates or inorganic proton donors pronouncedly reduced luciferin loading, whereas the effect of the counterion was modest. The remote-loading process barely affected the colloidal stability and drug retention of the liposomes, albeit with moderate luciferin-induced membrane perturbations. The correlation between luciferin loading, expressed as LE% and LC, and nLadd was established, and under our conditions the maximum LC was attained using an nLadd of around 2.6 μmol. Higher amounts of luciferin tend to pronouncedly perturb the liposome stability and luciferin retention. Our theoretical model furnishes a fair quantitative description of the correlation between nLadd and luciferin loading, and a membrane permeability coefficient for uncharged luciferin of 1 × 10-8 cm/s could be determined. We believe that our study will prove very useful to optimize the remote-loading strategies of moderately polar carboxylic acid drugs in general.

AB - We carried out a mechanistic study to characterize and optimize the remote loading of luciferin into preformed liposomes of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine/1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPC/DPPG) 7:3 mixtures. The influence of the loading agent (acetate, propionate, butyrate), the metal counterion (Na+, K+, Ca+2, Mg+2), and the initial extra-liposomal amount of luciferin (nLadd) on the luciferin Loading Efficiency (LE%) and luciferin-to-lipid weight ratio, i.e., Loading Capacity (LC), in the final formulation was determined. In addition, the effect of the loading process on the colloidal stability and phase behavior of the liposomes was monitored. Based on our experimental results, a theoretical model was developed to describe the course of luciferin remote loading. It was found that the highest luciferin loading was obtained with magnesium acetate. The use of longer aliphatic carboxylates or inorganic proton donors pronouncedly reduced luciferin loading, whereas the effect of the counterion was modest. The remote-loading process barely affected the colloidal stability and drug retention of the liposomes, albeit with moderate luciferin-induced membrane perturbations. The correlation between luciferin loading, expressed as LE% and LC, and nLadd was established, and under our conditions the maximum LC was attained using an nLadd of around 2.6 μmol. Higher amounts of luciferin tend to pronouncedly perturb the liposome stability and luciferin retention. Our theoretical model furnishes a fair quantitative description of the correlation between nLadd and luciferin loading, and a membrane permeability coefficient for uncharged luciferin of 1 × 10-8 cm/s could be determined. We believe that our study will prove very useful to optimize the remote-loading strategies of moderately polar carboxylic acid drugs in general.

KW - Carboxylate gradient

KW - Diffusion

KW - Drug encapsulation

KW - Liposome

KW - Luciferin remote loading

KW - Modeling

KW - Phospholipase A enzyme

U2 - 10.1016/j.ijpharm.2016.04.055

DO - 10.1016/j.ijpharm.2016.04.055

M3 - Journal article

C2 - 27163524

AN - SCOPUS:84973312219

VL - 508

SP - 128

EP - 134

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

IS - 1-2

ER -

ID: 230974097