New methods and reagents to improve the ferret model for human influenza infections

Research output: Contribution to conferencePosterResearch

Documents

  • Poster

    Final published version, 3.49 MB, PowerPoint presentation

  • Cyril Jean-Marie Martel
  • Svend Kirkeby
  • Bent Aasted
The ferret has been extensively used to study human influenza infections. However, its value as a model has suffered from the limited set of reagents and methods available for this animal. We have recently tested a large number of monoclonal antibodies cross-reacting with ferret CD markers (CD8, CD9, CD14, CD18, CD25, CD29, CD32, CD44, CD61, CD71, CD79b, CD88, CD104, CD172a and CD3) and cytokines (interferon-gamma, TNF-alpha, interleukine-4 and interleukine-8) for flow cytometry , as well as polyclonal antibodies cross-reacting with ferret immunoglobulins (IgA, IgG and IgM) for ELISA. Further improvements of the model will aim at establishing a reliable RT-PCR for ferret cytokines, as well as investigating the location of influenza receptors and viral particles in the upper and lower respiratory tract via immunohistochemistry
Original languageEnglish
Publication date2008
Publication statusPublished - 2008
EventGraduate School of Immunology 3. Annual Meeting: Interphases between adaptive and innate immunity in inflammation and autoimmunity - Helsingør, Denmark
Duration: 1 Oct 20083 Oct 2008
Conference number: 3

Conference

ConferenceGraduate School of Immunology 3. Annual Meeting: Interphases between adaptive and innate immunity in inflammation and autoimmunity
Number3
CountryDenmark
CityHelsingør
Period01/10/200803/10/2008

    Research areas

  • Former LIFE faculty - ferret, monoclonal antibodies, CD markers, cytokines, immunoglobulins

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