NADPH-dependent thioredoxin reductase and 2-Cys peroxiredoxins are needed for the protection of Mg-protoporphyrin monomethyl ester cyclase

Research output: Contribution to journalJournal articlepeer-review

  • Anne Stenbæk
  • Andreas Hansson
  • Ragna Peterson Wulff
  • Mats Hansson
  • Karl-Josef Dietz
  • Jensen, Poul Erik
Abstract: The chloroplast-localized NADPH-dependent thioredoxin reductase ( NTRC) has been found to be able to reduce hydrogen peroxide scavenging 2-Cys peroxiredoxins. We show that the Arabidopsis ntrc mutant is perturbed in chlorophyll biosynthesis and accumulate intermediates preceding protochlorophyllide formation. A specific involvement of NTRC during biosynthesis of protochlorophyllide is indicated from in vitro aerobic cyclase assays in which the conversion of Mg-protoporhyrin monomethyl ester into protochlorophyllide is stimulated by addition of the NTRC/2-Cys peroxiredoxin system. These findings support the hypothesis that this NADPH-dependent hydrogen peroxide scavenging system is particularly important during periods with limited reducing power from photosynthesis, e. g. under chloroplast biogenesis. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Original languageEnglish
JournalFEBS Letters
Volume582
Issue number18
Pages (from-to)2773-2778
Number of pages6
ISSN0014-5793
DOIs
Publication statusPublished - 2008

ID: 8854809