In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins

Research output: Contribution to journal › Journal article › Research › peer-review

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In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins. / Srimahaeak, Thanyaporn; Bianchi, Fernanda; Chlumsky, Ondrej; Larsen, Nadja; Jespersen, Lene.

In: Journal of Functional Foods, Vol. 79, 104395, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Srimahaeak, T, Bianchi, F, Chlumsky, O, Larsen, N & Jespersen, L 2021, 'In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins', Journal of Functional Foods, vol. 79, 104395. https://doi.org/10.1016/j.jff.2021.104395

APA

Srimahaeak, T., Bianchi, F., Chlumsky, O., Larsen, N., & Jespersen, L. (2021). In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins. Journal of Functional Foods, 79, [104395]. https://doi.org/10.1016/j.jff.2021.104395

Vancouver

Srimahaeak T, Bianchi F, Chlumsky O, Larsen N, Jespersen L. In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins. Journal of Functional Foods. 2021;79. 104395. https://doi.org/10.1016/j.jff.2021.104395

Author

Srimahaeak, Thanyaporn ; Bianchi, Fernanda ; Chlumsky, Ondrej ; Larsen, Nadja ; Jespersen, Lene. / In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins. In: Journal of Functional Foods. 2021 ; Vol. 79.

Bibtex

@article{3af7d12e57f2494a89954dd5a961cbfa,
title = "In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins",
abstract = "This study investigated the effect of four citrus pectins on adhesion of the probiotic Limosilactobacillus fermentum PCC and integrity of the intestinal epithelial monolayers using the Caco-2 cell model. Adhesion of L. fermentum PCC was enhanced (from 35% to 54–73%) in the presence of pectins with high and moderate degree of esterification (DE), whereas it was reduced (to 2%) by a pectin with low DE. Transepithelial electrical resistance (TEER) was significantly increased upon exposure to L. fermentum PCC alone (by 33%) and, to a greater extent, by combinations with pectins (by 44 – 48%), suggesting synergistic action of bacteria and pectins. Increases in TEER were related to transcriptional responses of the tight junction genes, encoding claudin-4 and claudin-2 proteins. The overall results indicated that pectins, when applied in synbiotic combination with L. fermentum PCC, have potential to improve bacterial adhesion and the intestinal epithelial barrier.",
keywords = "Adhesion, Caco-2 cells, LimosiLactobacillus fermentum PCC, Pectins, Tight junction",
author = "Thanyaporn Srimahaeak and Fernanda Bianchi and Ondrej Chlumsky and Nadja Larsen and Lene Jespersen",
year = "2021",
doi = "10.1016/j.jff.2021.104395",
language = "English",
volume = "79",
journal = "Journal of Functional Foods",
issn = "1756-4646",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins

AU - Srimahaeak, Thanyaporn

AU - Bianchi, Fernanda

AU - Chlumsky, Ondrej

AU - Larsen, Nadja

AU - Jespersen, Lene

PY - 2021

Y1 - 2021

N2 - This study investigated the effect of four citrus pectins on adhesion of the probiotic Limosilactobacillus fermentum PCC and integrity of the intestinal epithelial monolayers using the Caco-2 cell model. Adhesion of L. fermentum PCC was enhanced (from 35% to 54–73%) in the presence of pectins with high and moderate degree of esterification (DE), whereas it was reduced (to 2%) by a pectin with low DE. Transepithelial electrical resistance (TEER) was significantly increased upon exposure to L. fermentum PCC alone (by 33%) and, to a greater extent, by combinations with pectins (by 44 – 48%), suggesting synergistic action of bacteria and pectins. Increases in TEER were related to transcriptional responses of the tight junction genes, encoding claudin-4 and claudin-2 proteins. The overall results indicated that pectins, when applied in synbiotic combination with L. fermentum PCC, have potential to improve bacterial adhesion and the intestinal epithelial barrier.

AB - This study investigated the effect of four citrus pectins on adhesion of the probiotic Limosilactobacillus fermentum PCC and integrity of the intestinal epithelial monolayers using the Caco-2 cell model. Adhesion of L. fermentum PCC was enhanced (from 35% to 54–73%) in the presence of pectins with high and moderate degree of esterification (DE), whereas it was reduced (to 2%) by a pectin with low DE. Transepithelial electrical resistance (TEER) was significantly increased upon exposure to L. fermentum PCC alone (by 33%) and, to a greater extent, by combinations with pectins (by 44 – 48%), suggesting synergistic action of bacteria and pectins. Increases in TEER were related to transcriptional responses of the tight junction genes, encoding claudin-4 and claudin-2 proteins. The overall results indicated that pectins, when applied in synbiotic combination with L. fermentum PCC, have potential to improve bacterial adhesion and the intestinal epithelial barrier.

KW - Adhesion

KW - Caco-2 cells

KW - LimosiLactobacillus fermentum PCC

KW - Pectins

KW - Tight junction

U2 - 10.1016/j.jff.2021.104395

DO - 10.1016/j.jff.2021.104395

M3 - Journal article

AN - SCOPUS:85102607583

VL - 79

JO - Journal of Functional Foods

JF - Journal of Functional Foods

SN - 1756-4646

M1 - 104395

ER -

ID: 260404529