TY - JOUR

T1 - Interlamellar coupling of phospholipid bilayers in liposomes

T2 - An emergent property of lipid rearrangement

AU - Parry, Mikko J.

AU - Hagen, Morten

AU - Mouritsen, Ole G.

AU - Kinnunen, Paavo K.J.

AU - Alakoskela, Juha Matti I.

PY - 2010/4/6

Y1 - 2010/4/6

N2 - The thermal phase behaviors of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) large unilamellar vesicles (LUVs) and multilamellar vesicles (MLVs) were compared by fluorescence spectroscopy, using PPDPC (1-palmitoyl-2[10-(pyren-1- yl)]decanoyl-sn-glycero-3-phosphocholine) as a reporter, in parallel with differential scanning calorimetry (DSC). A striking difference is seen between MLVs and LUVs in the lateral organizational dynamics of PPDPC, in particular, below the main phase transition temperature Tm, with efficient clustering of PPDPC into fluid microdomains in the Lβ′ and Pβ′ (ripple) phases of DPPC MLVs. In the P β′ phase of MLVs, the probe is likely to become enriched in linear line defects, restricting intermolecular collisions to occur in a quasi one-dimensional system. In contrast, fluorescence and DSC data both suggest that the Pβ′ phase is not well-defined in LUVs. Fluorescence anisotropy for 1-palmitoyl-2-[3-(diphenylhexatrienyl)propanoyl]-sn- glycero-3-phosphocholine (DPH-PC) revealed similar acyl chain order for both LUVs and MLVs in the Lβ′ and Pβ′ phases. However, for MLVs with this probe, Tm determined from anisotropy was elevated by 0.7°, with higher anisotropy evident in the Lα phase compared to LUVs. These differences in the thermal phase behavior of the two types of liposomes are likely to derive from the augmented acyl chain order due to cooperative coupling of the lamellae of DPPC MLVs, thus manifesting in new, emerging material properties in the latter type of bilayer membrane assembly, as reflected in the organizational dynamics of the pyrene-labeled analogue.

AB - The thermal phase behaviors of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) large unilamellar vesicles (LUVs) and multilamellar vesicles (MLVs) were compared by fluorescence spectroscopy, using PPDPC (1-palmitoyl-2[10-(pyren-1- yl)]decanoyl-sn-glycero-3-phosphocholine) as a reporter, in parallel with differential scanning calorimetry (DSC). A striking difference is seen between MLVs and LUVs in the lateral organizational dynamics of PPDPC, in particular, below the main phase transition temperature Tm, with efficient clustering of PPDPC into fluid microdomains in the Lβ′ and Pβ′ (ripple) phases of DPPC MLVs. In the P β′ phase of MLVs, the probe is likely to become enriched in linear line defects, restricting intermolecular collisions to occur in a quasi one-dimensional system. In contrast, fluorescence and DSC data both suggest that the Pβ′ phase is not well-defined in LUVs. Fluorescence anisotropy for 1-palmitoyl-2-[3-(diphenylhexatrienyl)propanoyl]-sn- glycero-3-phosphocholine (DPH-PC) revealed similar acyl chain order for both LUVs and MLVs in the Lβ′ and Pβ′ phases. However, for MLVs with this probe, Tm determined from anisotropy was elevated by 0.7°, with higher anisotropy evident in the Lα phase compared to LUVs. These differences in the thermal phase behavior of the two types of liposomes are likely to derive from the augmented acyl chain order due to cooperative coupling of the lamellae of DPPC MLVs, thus manifesting in new, emerging material properties in the latter type of bilayer membrane assembly, as reflected in the organizational dynamics of the pyrene-labeled analogue.

UR - http://www.scopus.com/inward/record.url?scp=77950565326&partnerID=8YFLogxK

U2 - 10.1021/la9034547

DO - 10.1021/la9034547

M3 - Journal article

C2 - 20180577

AN - SCOPUS:77950565326

VL - 26

SP - 4909

EP - 4915

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 7

ER -