Indirect evidence for lipid-domain formation in the transition region of phospholipid bilayers by two-probe fluorescence energy transfer
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Indirect evidence for lipid-domain formation in the transition region of phospholipid bilayers by two-probe fluorescence energy transfer. / Pedersen, Solvejg; Jørgensen, Kent; Bækmark, Thomas R.; Mouritsen, Ole G.
In: Biophysical Journal, Vol. 71, No. 2, 08.1996, p. 554-560.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Indirect evidence for lipid-domain formation in the transition region of phospholipid bilayers by two-probe fluorescence energy transfer
AU - Pedersen, Solvejg
AU - Jørgensen, Kent
AU - Bækmark, Thomas R.
AU - Mouritsen, Ole G.
PY - 1996/8
Y1 - 1996/8
N2 - The fluorescence energy transfer between two lipid probes, N-(7- nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3- phosphoethanolamine (donor) and N-(Lissamine rhodamine B sulfonyl)-1,2- dihexadecanoyl-sn-glycero-3-phosphoethanolamine (acceptor), incorporated into 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine unilamellar and multilamellar lipid bilayers, is studied in the temperature region of the main phase transition. The two probes display different relative solubilities in the gel and fluid lipid-bilayer phases. A distinct maximum in the fluorescence intensity of the donor is observed in the transition region, indicating that the two probes are demixing and hence increasing their average separation. The observation is interpreted in terms of dynamic segregation of the two probes into coexisting gel and fluid lipid domains that are formed dynamically in the transition region due to strong density fluctuations. The interpretation of the experimental observations is supported by a detailed theoretical calculation using computer simulation of a microscopic model that takes full account of diffusion of the two probes and the fluctuations of gel and fluid lipid domains characteristic of the main phase transition.
AB - The fluorescence energy transfer between two lipid probes, N-(7- nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3- phosphoethanolamine (donor) and N-(Lissamine rhodamine B sulfonyl)-1,2- dihexadecanoyl-sn-glycero-3-phosphoethanolamine (acceptor), incorporated into 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine unilamellar and multilamellar lipid bilayers, is studied in the temperature region of the main phase transition. The two probes display different relative solubilities in the gel and fluid lipid-bilayer phases. A distinct maximum in the fluorescence intensity of the donor is observed in the transition region, indicating that the two probes are demixing and hence increasing their average separation. The observation is interpreted in terms of dynamic segregation of the two probes into coexisting gel and fluid lipid domains that are formed dynamically in the transition region due to strong density fluctuations. The interpretation of the experimental observations is supported by a detailed theoretical calculation using computer simulation of a microscopic model that takes full account of diffusion of the two probes and the fluctuations of gel and fluid lipid domains characteristic of the main phase transition.
UR - http://www.scopus.com/inward/record.url?scp=0029738338&partnerID=8YFLogxK
U2 - 10.1016/S0006-3495(96)79279-2
DO - 10.1016/S0006-3495(96)79279-2
M3 - Journal article
C2 - 8842195
AN - SCOPUS:0029738338
VL - 71
SP - 554
EP - 560
JO - Biophysical Society. Annual Meeting. Abstracts
JF - Biophysical Society. Annual Meeting. Abstracts
SN - 0523-6800
IS - 2
ER -
ID: 236888299