Identification of Potential Citrate Metabolism Pathways in Carnobacterium maltaromaticum

Research output: Contribution to journal › Journal article › peer-review

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Identification of Potential Citrate Metabolism Pathways in Carnobacterium maltaromaticum. / Li, Heng; Ramia, Nancy E; Borges, Frédéric; Revol-Junelles, Anne-Marie; Vogensen, Finn Kvist; Leisner, Jørgen J.

In: Microorganisms, Vol. 9, No. 10, 2169, 2021.

Research output: Contribution to journal › Journal article › peer-review

Harvard

Li, H, Ramia, NE, Borges, F, Revol-Junelles, A-M, Vogensen, FK & Leisner, JJ 2021, 'Identification of Potential Citrate Metabolism Pathways in Carnobacterium maltaromaticum', Microorganisms, vol. 9, no. 10, 2169. https://doi.org/10.3390/microorganisms9102169

APA

Li, H., Ramia, N. E., Borges, F., Revol-Junelles, A-M., Vogensen, F. K., & Leisner, J. J. (2021). Identification of Potential Citrate Metabolism Pathways in Carnobacterium maltaromaticum. Microorganisms, 9(10), [2169]. https://doi.org/10.3390/microorganisms9102169

Vancouver

Li H, Ramia NE, Borges F, Revol-Junelles A-M, Vogensen FK, Leisner JJ. Identification of Potential Citrate Metabolism Pathways in Carnobacterium maltaromaticum. Microorganisms. 2021;9(10). 2169. https://doi.org/10.3390/microorganisms9102169

Author

Li, Heng ; Ramia, Nancy E ; Borges, Frédéric ; Revol-Junelles, Anne-Marie ; Vogensen, Finn Kvist ; Leisner, Jørgen J. / Identification of Potential Citrate Metabolism Pathways in Carnobacterium maltaromaticum. In: Microorganisms. 2021 ; Vol. 9, No. 10.

Bibtex

@article{426f2efeaa8c45a391692a96c91f0159,
title = "Identification of Potential Citrate Metabolism Pathways in Carnobacterium maltaromaticum",
abstract = "In the present study, we describe the identification of potential citrate metabolism pathways for the lactic acid bacterium (LAB) Carnobacterium maltaromaticum. A phenotypic assay indicated that four of six C. maltaromaticum strains showed weak (Cm 6-1 and ATCC 35586) or even delayed (Cm 3-1 and Cm 5-1) citrate utilization activity. The remaining two strains, Cm 4-1 and Cm 1-2 gave negative results. Additional analysis showed no or very limited utilization of citrate in media containing 1% glucose and 22 or 30 mM citrate and inoculated with Cm 6-1 or ATCC 35586. Two potential pathways of citrate metabolism were identified by bioinformatics analyses in C. maltaromaticum including either oxaloacetate (pathway 1) or tricarboxylic compounds such as isocitrate and α-ketoglutarate (pathway 2) as intermediates. Genes encoding pathway 1 were present in two out of six strains while pathway 2 included genes present in all six strains. The two potential citrate metabolism pathways in C. maltaromaticum may potentially affect the sensory profiles of milk and soft cheeses subjected to growth with this species.",
author = "Heng Li and Ramia, {Nancy E} and Fr{\'e}d{\'e}ric Borges and Anne-Marie Revol-Junelles and Vogensen, {Finn Kvist} and Leisner, {J{\o}rgen J}",
year = "2021",
doi = "10.3390/microorganisms9102169",
language = "English",
volume = "9",
journal = "Microorganisms",
issn = "2076-2607",
publisher = "M D P I AG",
number = "10",

}

RIS

TY - JOUR

T1 - Identification of Potential Citrate Metabolism Pathways in Carnobacterium maltaromaticum

AU - Li, Heng

AU - Ramia, Nancy E

AU - Borges, Frédéric

AU - Revol-Junelles, Anne-Marie

AU - Vogensen, Finn Kvist

AU - Leisner, Jørgen J

PY - 2021

Y1 - 2021

N2 - In the present study, we describe the identification of potential citrate metabolism pathways for the lactic acid bacterium (LAB) Carnobacterium maltaromaticum. A phenotypic assay indicated that four of six C. maltaromaticum strains showed weak (Cm 6-1 and ATCC 35586) or even delayed (Cm 3-1 and Cm 5-1) citrate utilization activity. The remaining two strains, Cm 4-1 and Cm 1-2 gave negative results. Additional analysis showed no or very limited utilization of citrate in media containing 1% glucose and 22 or 30 mM citrate and inoculated with Cm 6-1 or ATCC 35586. Two potential pathways of citrate metabolism were identified by bioinformatics analyses in C. maltaromaticum including either oxaloacetate (pathway 1) or tricarboxylic compounds such as isocitrate and α-ketoglutarate (pathway 2) as intermediates. Genes encoding pathway 1 were present in two out of six strains while pathway 2 included genes present in all six strains. The two potential citrate metabolism pathways in C. maltaromaticum may potentially affect the sensory profiles of milk and soft cheeses subjected to growth with this species.

AB - In the present study, we describe the identification of potential citrate metabolism pathways for the lactic acid bacterium (LAB) Carnobacterium maltaromaticum. A phenotypic assay indicated that four of six C. maltaromaticum strains showed weak (Cm 6-1 and ATCC 35586) or even delayed (Cm 3-1 and Cm 5-1) citrate utilization activity. The remaining two strains, Cm 4-1 and Cm 1-2 gave negative results. Additional analysis showed no or very limited utilization of citrate in media containing 1% glucose and 22 or 30 mM citrate and inoculated with Cm 6-1 or ATCC 35586. Two potential pathways of citrate metabolism were identified by bioinformatics analyses in C. maltaromaticum including either oxaloacetate (pathway 1) or tricarboxylic compounds such as isocitrate and α-ketoglutarate (pathway 2) as intermediates. Genes encoding pathway 1 were present in two out of six strains while pathway 2 included genes present in all six strains. The two potential citrate metabolism pathways in C. maltaromaticum may potentially affect the sensory profiles of milk and soft cheeses subjected to growth with this species.

U2 - 10.3390/microorganisms9102169

DO - 10.3390/microorganisms9102169

M3 - Journal article

C2 - 34683489

VL - 9

JO - Microorganisms

JF - Microorganisms

SN - 2076-2607

IS - 10

M1 - 2169

ER -

ID: 282950186