Hybrid coating of alginate microbeads based on protein-biopolymer multilayers for encapsulation of probiotics
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Hybrid coating of alginate microbeads based on protein-biopolymer multilayers for encapsulation of probiotics. / Yücel Falco, Cigdem; Amadei, Federico; Dhayal, Surender K.; Cárdenas, Marité; Tanaka, Motomu; Risbo, Jens.
In: Biotechnology Progress, Vol. 35, No. 3, e2806, 2019.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Hybrid coating of alginate microbeads based on protein-biopolymer multilayers for encapsulation of probiotics
AU - Yücel Falco, Cigdem
AU - Amadei, Federico
AU - Dhayal, Surender K.
AU - Cárdenas, Marité
AU - Tanaka, Motomu
AU - Risbo, Jens
PY - 2019
Y1 - 2019
N2 - A hybrid coating based on multilayers of proteins and biopolymers was developed to enhance the protection performance of alginate microbeads against acidic conditions for delivery of probiotics (Lactobacillus rhamnosus GG). Zeta potential measurements and quartz crystal microbalance with dissipation confirmed layer-by-layer deposition of protein-polymer layers. The stability of protein-based coatings during simulated gastric fluid (SGF) treatment was monitored by microscopy. Protein-coated microbeads were partially dismantled, whereas polymer-coated microbeads were intact after a sequential treatment in simulated gastric and intestinal fluids. This suggests that hybrid formulation offers an advantage over the coatings based on biopolymer multilayers in terms of better release of bacteria. Uncoated alginate microbeads completely dissolved and could not protect bacteria after SGF treatment whereas microbeads with hybrid coating showed increased physical stability and a modest decrease of culturability of 3.8 log units. Therefore, this work provides a concept for future protein-based hybrid coatings for bacterial delivery systems.
AB - A hybrid coating based on multilayers of proteins and biopolymers was developed to enhance the protection performance of alginate microbeads against acidic conditions for delivery of probiotics (Lactobacillus rhamnosus GG). Zeta potential measurements and quartz crystal microbalance with dissipation confirmed layer-by-layer deposition of protein-polymer layers. The stability of protein-based coatings during simulated gastric fluid (SGF) treatment was monitored by microscopy. Protein-coated microbeads were partially dismantled, whereas polymer-coated microbeads were intact after a sequential treatment in simulated gastric and intestinal fluids. This suggests that hybrid formulation offers an advantage over the coatings based on biopolymer multilayers in terms of better release of bacteria. Uncoated alginate microbeads completely dissolved and could not protect bacteria after SGF treatment whereas microbeads with hybrid coating showed increased physical stability and a modest decrease of culturability of 3.8 log units. Therefore, this work provides a concept for future protein-based hybrid coatings for bacterial delivery systems.
KW - alginate
KW - chitosan
KW - lactoferrin
KW - layer-by-layer
KW - microencapsulation of probiotics
U2 - 10.1002/btpr.2806
DO - 10.1002/btpr.2806
M3 - Journal article
C2 - 30884190
AN - SCOPUS:85063428235
VL - 35
JO - Biotechnology Progress
JF - Biotechnology Progress
SN - 8756-7938
IS - 3
M1 - e2806
ER -
ID: 217995792