Host genetic requirements for DNA release of lactococcal phage TP901-1
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Host genetic requirements for DNA release of lactococcal phage TP901-1. / Ruiz-Cruz, Sofía; Erazo Garzon, Andrea; Kelleher, Philip; Bottacini, Francesca; Breum, Solvej Østergaard; Neve, Horst; Heller, Knut J.; Vogensen, Finn K.; Palussière, Simon; Courtin, Pascal; Chapot-Chartier, Marie-Pierre; Vinogradov, Evgeny; Sadovskaya, Irina; Mahony, Jennifer; van Sinderen, Douwe.
In: Microbial Biotechnology, Vol. 15, No. 12, 2022, p. 2875-2889.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Host genetic requirements for DNA release of lactococcal phage TP901-1
AU - Ruiz-Cruz, Sofía
AU - Erazo Garzon, Andrea
AU - Kelleher, Philip
AU - Bottacini, Francesca
AU - Breum, Solvej Østergaard
AU - Neve, Horst
AU - Heller, Knut J.
AU - Vogensen, Finn K.
AU - Palussière, Simon
AU - Courtin, Pascal
AU - Chapot-Chartier, Marie-Pierre
AU - Vinogradov, Evgeny
AU - Sadovskaya, Irina
AU - Mahony, Jennifer
AU - van Sinderen, Douwe
N1 - Publisher Copyright: © 2022 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd.
PY - 2022
Y1 - 2022
N2 - The first step in phage infection is the recognition of, and adsorption to, a receptor located on the host cell surface. This reversible host adsorption step is commonly followed by an irreversible event, which involves phage DNA delivery or release into the bacterial cytoplasm. The molecular components that trigger this latter event are unknown for most phages of Gram-positive bacteria. In the current study, we present a comparative genome analysis of three mutants of Lactococcus cremoris 3107, which are resistant to the P335 group phage TP901-1 due to mutations that affect TP901-1 DNA release. Through genetic complementation and phage infection assays, a predicted lactococcal three-component glycosylation system (TGS) was shown to be required for TP901-1 infection. Major cell wall saccharidic components were analysed, but no differences were found. However, heterologous gene expression experiments indicate that this TGS is involved in the glucosylation of a cell envelope-associated component that triggers TP901-1 DNA release. To date, a saccharide modification has not been implicated in the DNA delivery process of a Gram-positive infecting phage.
AB - The first step in phage infection is the recognition of, and adsorption to, a receptor located on the host cell surface. This reversible host adsorption step is commonly followed by an irreversible event, which involves phage DNA delivery or release into the bacterial cytoplasm. The molecular components that trigger this latter event are unknown for most phages of Gram-positive bacteria. In the current study, we present a comparative genome analysis of three mutants of Lactococcus cremoris 3107, which are resistant to the P335 group phage TP901-1 due to mutations that affect TP901-1 DNA release. Through genetic complementation and phage infection assays, a predicted lactococcal three-component glycosylation system (TGS) was shown to be required for TP901-1 infection. Major cell wall saccharidic components were analysed, but no differences were found. However, heterologous gene expression experiments indicate that this TGS is involved in the glucosylation of a cell envelope-associated component that triggers TP901-1 DNA release. To date, a saccharide modification has not been implicated in the DNA delivery process of a Gram-positive infecting phage.
U2 - 10.1111/1751-7915.14156
DO - 10.1111/1751-7915.14156
M3 - Journal article
C2 - 36259418
AN - SCOPUS:85140100547
VL - 15
SP - 2875
EP - 2889
JO - Microbial Biotechnology
JF - Microbial Biotechnology
SN - 1751-7907
IS - 12
ER -
ID: 324313207