Genetic analysis of Trichuris suis and Trichuris trichiura recovered from humans and pigs in a sympatric setting in Uganda
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Genetic analysis of Trichuris suis and Trichuris trichiura recovered from humans and pigs in a sympatric setting in Uganda. / Nissen, Sofie; Al-Jubury, Azmi; Hansen, Tina Vicky Alstrup; Olsen, Annette; Christensen, Henrik; Thamsborg, Stig Milan; Nejsum, Peter.
In: Veterinary Parasitology, Vol. 188, No. 1-2, 2012, p. 68-77.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Genetic analysis of Trichuris suis and Trichuris trichiura recovered from humans and pigs in a sympatric setting in Uganda
AU - Nissen, Sofie
AU - Al-Jubury, Azmi
AU - Hansen, Tina Vicky Alstrup
AU - Olsen, Annette
AU - Christensen, Henrik
AU - Thamsborg, Stig Milan
AU - Nejsum, Peter
PY - 2012
Y1 - 2012
N2 - The whipworms Trichuris trichiura and Trichuris suis in humans and pigs, respectively, arebelieved to be two different species yet closely related. Morphologically, adult worms, eggsand larvae of the two species are indistinguishable. The aim of this study was to examine the genetic variation of Trichuris sp. mainly recovered from natural infected pigs and humans. Worm material isolated from humans and pigs living in the same geographical region in Uganda were analyzed by PCR, cloning and sequencing. Measurements of morphometric characters were also performed. The analysis of the ITS-2 (internal transcribed spacer) region showed a high genetic variation in the human-derived worms with two sequence types, designated type 1 and type 2, differing with up to 45%, the type 2 being identical to the sequence found in pig-derived worms. A single human-derived worm showed exclusively the type 2-genotype (T. suis-type) and three cases of ‘heterozygote’ worms in humans were identified. However, the analysis showed that sympatric Trichuris primarily assorted with host origin. Sequence analysis of a part of the genetically conserved ¿-tubulin gene confirmed two separate populations/species but also showed that the ‘heterozygote’ worms had a T. suis-like ¿-tubulin gene. A PCR-RFLP on the ITS-2 region was developed, that could distinguish between worms of the pig, human and ‘heterozygote’ type. The data suggest that Trichuris in pigs and humans belong to two different populations (i.e. are two different species). However, the data presented also suggest that cross-infections of humans with T. suis takes place. Further studies on sympatric Trichuris populations are highly warranted in order to explore transmission dynamics and unravel the zoonotic potential of T. suis.
AB - The whipworms Trichuris trichiura and Trichuris suis in humans and pigs, respectively, arebelieved to be two different species yet closely related. Morphologically, adult worms, eggsand larvae of the two species are indistinguishable. The aim of this study was to examine the genetic variation of Trichuris sp. mainly recovered from natural infected pigs and humans. Worm material isolated from humans and pigs living in the same geographical region in Uganda were analyzed by PCR, cloning and sequencing. Measurements of morphometric characters were also performed. The analysis of the ITS-2 (internal transcribed spacer) region showed a high genetic variation in the human-derived worms with two sequence types, designated type 1 and type 2, differing with up to 45%, the type 2 being identical to the sequence found in pig-derived worms. A single human-derived worm showed exclusively the type 2-genotype (T. suis-type) and three cases of ‘heterozygote’ worms in humans were identified. However, the analysis showed that sympatric Trichuris primarily assorted with host origin. Sequence analysis of a part of the genetically conserved ¿-tubulin gene confirmed two separate populations/species but also showed that the ‘heterozygote’ worms had a T. suis-like ¿-tubulin gene. A PCR-RFLP on the ITS-2 region was developed, that could distinguish between worms of the pig, human and ‘heterozygote’ type. The data suggest that Trichuris in pigs and humans belong to two different populations (i.e. are two different species). However, the data presented also suggest that cross-infections of humans with T. suis takes place. Further studies on sympatric Trichuris populations are highly warranted in order to explore transmission dynamics and unravel the zoonotic potential of T. suis.
KW - Former LIFE faculty
KW - Zoonosis
KW - Trichuris suis
KW - Trichuris trichiura
KW - ITS-2
KW - PCR-RFLP
KW - ß-tubulin
KW - Cross-infection
U2 - 10.1016/j.vetpar.2012.03.004
DO - 10.1016/j.vetpar.2012.03.004
M3 - Journal article
C2 - 22494938
VL - 188
SP - 68
EP - 77
JO - Veterinary Parasitology
JF - Veterinary Parasitology
SN - 0304-4017
IS - 1-2
ER -
ID: 40395390