Generation of short-chained granular corn starch by maltogenic α-amylase and transglucosidase treatment
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Generation of short-chained granular corn starch by maltogenic α-amylase and transglucosidase treatment. / Zhong, Yuyue; Keeratiburana, Thewika; Kirkensgaard, Jacob Judas Kain; Khakimov, Bekzod; Blennow, Andreas; Hansen, Aleksander Riise.
In: Carbohydrate Polymers, Vol. 251, 117056, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Generation of short-chained granular corn starch by maltogenic α-amylase and transglucosidase treatment
AU - Zhong, Yuyue
AU - Keeratiburana, Thewika
AU - Kirkensgaard, Jacob Judas Kain
AU - Khakimov, Bekzod
AU - Blennow, Andreas
AU - Hansen, Aleksander Riise
PY - 2021
Y1 - 2021
N2 - We describe a method for permitting efficient modification by transglucosidase (TGA), from glycoside hydrolase family 31 (GH31), sequentially after the pre-treatment by maltogenic α-amylases (MA) from GH13. TGA treatment without MA pre-treatment had negligible effects on native starch, while TGA treatment with MA pre-treatment resulted in porous granules and increased permeability to enzymes. MA→TGA treatments lead to decreased molecular size of amylopectin molecules, increased α-1,6 branching, and increased amounts of amylopectin chains with the degree of polymerization (DP)<10 and decreased amounts of DP 10-28 after debranching. Wide-angle X-ray scattering (WAXS) data showed a general decrease in crystallinity except for a long term (20 h) TGA post-treatment which increased the relative crystallinity back to normal. MA→TGA treatment significantly lowered the starch retrogradation of starch and retarded the increase of storage- and loss moduli during storage. This work demonstrates the potential of sequential addition of starch active enzymes to obtain granular starch with improved functionality.
AB - We describe a method for permitting efficient modification by transglucosidase (TGA), from glycoside hydrolase family 31 (GH31), sequentially after the pre-treatment by maltogenic α-amylases (MA) from GH13. TGA treatment without MA pre-treatment had negligible effects on native starch, while TGA treatment with MA pre-treatment resulted in porous granules and increased permeability to enzymes. MA→TGA treatments lead to decreased molecular size of amylopectin molecules, increased α-1,6 branching, and increased amounts of amylopectin chains with the degree of polymerization (DP)<10 and decreased amounts of DP 10-28 after debranching. Wide-angle X-ray scattering (WAXS) data showed a general decrease in crystallinity except for a long term (20 h) TGA post-treatment which increased the relative crystallinity back to normal. MA→TGA treatment significantly lowered the starch retrogradation of starch and retarded the increase of storage- and loss moduli during storage. This work demonstrates the potential of sequential addition of starch active enzymes to obtain granular starch with improved functionality.
KW - Maltogenic α-amylase
KW - Solid-state modification
KW - Starch
KW - Transglucosidase
U2 - 10.1016/j.carbpol.2020.117056
DO - 10.1016/j.carbpol.2020.117056
M3 - Journal article
C2 - 33142608
AN - SCOPUS:85090552700
VL - 251
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
SN - 0144-8617
M1 - 117056
ER -
ID: 249767084