From lanosterol to cholesterol: Structural evolution and differential effects on lipid bilayers

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From lanosterol to cholesterol : Structural evolution and differential effects on lipid bilayers. / Miao, Ling; Nielsen, Morten; Thewalt, Jenifer; Ipsen, John H.; Bloom, Myer; Zuckermann, Martin J.; Mouritsen, Ole G.

In: Biophysical Journal, Vol. 82, No. 3, 01.01.2002, p. 1429-1444.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Miao, L, Nielsen, M, Thewalt, J, Ipsen, JH, Bloom, M, Zuckermann, MJ & Mouritsen, OG 2002, 'From lanosterol to cholesterol: Structural evolution and differential effects on lipid bilayers', Biophysical Journal, vol. 82, no. 3, pp. 1429-1444. https://doi.org/10.1016/S0006-3495(02)75497-0

APA

Miao, L., Nielsen, M., Thewalt, J., Ipsen, J. H., Bloom, M., Zuckermann, M. J., & Mouritsen, O. G. (2002). From lanosterol to cholesterol: Structural evolution and differential effects on lipid bilayers. Biophysical Journal, 82(3), 1429-1444. https://doi.org/10.1016/S0006-3495(02)75497-0

Vancouver

Miao L, Nielsen M, Thewalt J, Ipsen JH, Bloom M, Zuckermann MJ et al. From lanosterol to cholesterol: Structural evolution and differential effects on lipid bilayers. Biophysical Journal. 2002 Jan 1;82(3):1429-1444. https://doi.org/10.1016/S0006-3495(02)75497-0

Author

Miao, Ling ; Nielsen, Morten ; Thewalt, Jenifer ; Ipsen, John H. ; Bloom, Myer ; Zuckermann, Martin J. ; Mouritsen, Ole G. / From lanosterol to cholesterol : Structural evolution and differential effects on lipid bilayers. In: Biophysical Journal. 2002 ; Vol. 82, No. 3. pp. 1429-1444.

Bibtex

@article{745d47a3f83c4ca8915de85e1ecbf136,
title = "From lanosterol to cholesterol: Structural evolution and differential effects on lipid bilayers",
abstract = "Cholesterol is an important molecular component of the plasma membranes of mammalian cells. Its precursor in the sterol biosynthetic pathway, lanosterol, has been argued by Konrad Bloch (Bloch, K. 1965. Science. 150:19-28; 1983. CRC Crit. Rev. Biochem. 14:47-92; 1994. Blonds in Venetian Paintings, the Nine-Banded Armadillo, and Other Essays in Biochemistry. Yale University Press, New Haven, CT.) to also be a precursor in the molecular evolution of cholesterol. We present a comparative study of the effects of cholesterol and lanosterol on molecular conformational order and phase equilibria of lipid-bilayer membranes. By using deuterium NMR spectroscopy on multilamellar lipid-sterol systems in combination with Monte Carlo simulations of microscopic models of lipid-sterol interactions, we demonstrate that the evolution in the molecular chemistry from lanosterol to cholesterol is manifested in the model lipid-sterol membranes by an increase in the ability of the sterols to promote and stabilize a particular membrane phase, the liquid-ordered phase, and to induce collective order in the acyl-chain conformations of lipid molecules. We also discuss the biological relevance of our results, in particular in the context of membrane domains and rafts.",
author = "Ling Miao and Morten Nielsen and Jenifer Thewalt and Ipsen, {John H.} and Myer Bloom and Zuckermann, {Martin J.} and Mouritsen, {Ole G.}",
year = "2002",
month = jan,
day = "1",
doi = "10.1016/S0006-3495(02)75497-0",
language = "English",
volume = "82",
pages = "1429--1444",
journal = "Biophysical Society. Annual Meeting. Abstracts",
issn = "0523-6800",
publisher = "Biophysical Society",
number = "3",

}

RIS

TY - JOUR

T1 - From lanosterol to cholesterol

T2 - Structural evolution and differential effects on lipid bilayers

AU - Miao, Ling

AU - Nielsen, Morten

AU - Thewalt, Jenifer

AU - Ipsen, John H.

AU - Bloom, Myer

AU - Zuckermann, Martin J.

AU - Mouritsen, Ole G.

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Cholesterol is an important molecular component of the plasma membranes of mammalian cells. Its precursor in the sterol biosynthetic pathway, lanosterol, has been argued by Konrad Bloch (Bloch, K. 1965. Science. 150:19-28; 1983. CRC Crit. Rev. Biochem. 14:47-92; 1994. Blonds in Venetian Paintings, the Nine-Banded Armadillo, and Other Essays in Biochemistry. Yale University Press, New Haven, CT.) to also be a precursor in the molecular evolution of cholesterol. We present a comparative study of the effects of cholesterol and lanosterol on molecular conformational order and phase equilibria of lipid-bilayer membranes. By using deuterium NMR spectroscopy on multilamellar lipid-sterol systems in combination with Monte Carlo simulations of microscopic models of lipid-sterol interactions, we demonstrate that the evolution in the molecular chemistry from lanosterol to cholesterol is manifested in the model lipid-sterol membranes by an increase in the ability of the sterols to promote and stabilize a particular membrane phase, the liquid-ordered phase, and to induce collective order in the acyl-chain conformations of lipid molecules. We also discuss the biological relevance of our results, in particular in the context of membrane domains and rafts.

AB - Cholesterol is an important molecular component of the plasma membranes of mammalian cells. Its precursor in the sterol biosynthetic pathway, lanosterol, has been argued by Konrad Bloch (Bloch, K. 1965. Science. 150:19-28; 1983. CRC Crit. Rev. Biochem. 14:47-92; 1994. Blonds in Venetian Paintings, the Nine-Banded Armadillo, and Other Essays in Biochemistry. Yale University Press, New Haven, CT.) to also be a precursor in the molecular evolution of cholesterol. We present a comparative study of the effects of cholesterol and lanosterol on molecular conformational order and phase equilibria of lipid-bilayer membranes. By using deuterium NMR spectroscopy on multilamellar lipid-sterol systems in combination with Monte Carlo simulations of microscopic models of lipid-sterol interactions, we demonstrate that the evolution in the molecular chemistry from lanosterol to cholesterol is manifested in the model lipid-sterol membranes by an increase in the ability of the sterols to promote and stabilize a particular membrane phase, the liquid-ordered phase, and to induce collective order in the acyl-chain conformations of lipid molecules. We also discuss the biological relevance of our results, in particular in the context of membrane domains and rafts.

UR - http://www.scopus.com/inward/record.url?scp=0036198064&partnerID=8YFLogxK

U2 - 10.1016/S0006-3495(02)75497-0

DO - 10.1016/S0006-3495(02)75497-0

M3 - Journal article

C2 - 11867458

AN - SCOPUS:0036198064

VL - 82

SP - 1429

EP - 1444

JO - Biophysical Society. Annual Meeting. Abstracts

JF - Biophysical Society. Annual Meeting. Abstracts

SN - 0523-6800

IS - 3

ER -

ID: 230987322