TY - JOUR

T1 - Electro-membrane fractionation of antioxidant peptides from protein hydrolysates of rainbow trout (Oncorhynchus mykiss) byproducts

AU - Suwal, Shyam

AU - Ketnawa, Sunantha

AU - Liceaga, Andrea M.

AU - Huang, Jen-Yi

PY - 2018

Y1 - 2018

N2 - Fish protein hydrolysates are an important source of antioxidant peptides. Electrically driven membrane fractionation called electrodialysis with filtration membrane (EDFM) is a separation technology based on molecular charge and mass, which can fractionate active peptides from complex hydrolysates. This work aimed to evaluate the feasibility of sequential EDFM process for separation of cationic (CP) and anionic (AP) peptides from rainbow trout frame protein hydrolysate, and determine their antioxidant properties. The concentrations of CP and AP increased in the recovery solution, reaching 156 and 85 μg/mL, respectively, after 4-hour treatment, with migration rates of 19.55 ± 2.19 and 10.94 ± 0.39 g/m2h. The CP separation was approximately 50% energy efficient than AP. Both CP and AP fractions were enriched with peptides with DPPH and ABTS radical scavenging properties. The results showed that two-step EDFM process is feasible for recovery and concentration of antioxidant peptides from rainbow trout protein hydrolysate. Industrial relevance The electro-membrane fractionation developed in this study is a two-step process, which is able to selectively separate antioxidant peptides from enzymatic protein hydrolysates based on charge and size. With this particular raw material as the feed, we have shown that using this approach lead to the highest peptide migration rate and a significant improvement in antioxidant activities of both peptide fractions. In addition, this technique is very selective, and environmental friendly as it requires no use of solvent and consumes less energy compared to conventional chromatographic techniques, and thus can be used as a green technology for the fractionation of bioactive peptides from a complex mixture of protein hydrolysates.

AB - Fish protein hydrolysates are an important source of antioxidant peptides. Electrically driven membrane fractionation called electrodialysis with filtration membrane (EDFM) is a separation technology based on molecular charge and mass, which can fractionate active peptides from complex hydrolysates. This work aimed to evaluate the feasibility of sequential EDFM process for separation of cationic (CP) and anionic (AP) peptides from rainbow trout frame protein hydrolysate, and determine their antioxidant properties. The concentrations of CP and AP increased in the recovery solution, reaching 156 and 85 μg/mL, respectively, after 4-hour treatment, with migration rates of 19.55 ± 2.19 and 10.94 ± 0.39 g/m2h. The CP separation was approximately 50% energy efficient than AP. Both CP and AP fractions were enriched with peptides with DPPH and ABTS radical scavenging properties. The results showed that two-step EDFM process is feasible for recovery and concentration of antioxidant peptides from rainbow trout protein hydrolysate. Industrial relevance The electro-membrane fractionation developed in this study is a two-step process, which is able to selectively separate antioxidant peptides from enzymatic protein hydrolysates based on charge and size. With this particular raw material as the feed, we have shown that using this approach lead to the highest peptide migration rate and a significant improvement in antioxidant activities of both peptide fractions. In addition, this technique is very selective, and environmental friendly as it requires no use of solvent and consumes less energy compared to conventional chromatographic techniques, and thus can be used as a green technology for the fractionation of bioactive peptides from a complex mixture of protein hydrolysates.

KW - Anionic peptides

KW - Antioxidant properties

KW - Cationic peptides

KW - Electrodialysis with filtration membrane

KW - Fish protein hydrolysate

U2 - 10.1016/j.ifset.2017.08.016

DO - 10.1016/j.ifset.2017.08.016

M3 - Journal article

VL - 45

SP - 122

EP - 131

JO - Innovative Food Science and Emerging Technologies

JF - Innovative Food Science and Emerging Technologies

SN - 1466-8564

ER -