DNA of Dientamoeba fragilis detected within surface-sterilized eggs of Enterobius vermicularis

Research output: Contribution to journalJournal articlepeer-review

Standard

DNA of Dientamoeba fragilis detected within surface-sterilized eggs of Enterobius vermicularis. / Röser, Dennis; Nejsum, Peter; Carlsgart, Anne Josefine; Nielsen, Henrik Vedel; Stensvold, Christen Rune.

In: Experimental Parasitology, Vol. 133, No. 1, 2013, p. 57-61.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Röser, D, Nejsum, P, Carlsgart, AJ, Nielsen, HV & Stensvold, CR 2013, 'DNA of Dientamoeba fragilis detected within surface-sterilized eggs of Enterobius vermicularis', Experimental Parasitology, vol. 133, no. 1, pp. 57-61. https://doi.org/10.1016/j.exppara.2012.10.009

APA

Röser, D., Nejsum, P., Carlsgart, A. J., Nielsen, H. V., & Stensvold, C. R. (2013). DNA of Dientamoeba fragilis detected within surface-sterilized eggs of Enterobius vermicularis. Experimental Parasitology, 133(1), 57-61. https://doi.org/10.1016/j.exppara.2012.10.009

Vancouver

Röser D, Nejsum P, Carlsgart AJ, Nielsen HV, Stensvold CR. DNA of Dientamoeba fragilis detected within surface-sterilized eggs of Enterobius vermicularis. Experimental Parasitology. 2013;133(1):57-61. https://doi.org/10.1016/j.exppara.2012.10.009

Author

Röser, Dennis ; Nejsum, Peter ; Carlsgart, Anne Josefine ; Nielsen, Henrik Vedel ; Stensvold, Christen Rune. / DNA of Dientamoeba fragilis detected within surface-sterilized eggs of Enterobius vermicularis. In: Experimental Parasitology. 2013 ; Vol. 133, No. 1. pp. 57-61.

Bibtex

@article{206dea15c1834d9fa1cbccf52e60e22c,
title = "DNA of Dientamoeba fragilis detected within surface-sterilized eggs of Enterobius vermicularis",
abstract = "With no evidence of a cyst stage, the mode of transmission of Dientamoeba fragilis, an intestinal protozoon of common occurrence and suggested pathogenicity, is incompletely known. Numerous studies have suggested that eggs of intestinal nematodes, primarily Enterobius vermicularis (pinworm), can serve as vectors for D. fragilis, although attempts to culture D. fragilis from pinworm eggs have been unsuccessful and data from epidemiological studies on D. fragilis/pinworm co-infection have been conflicting. The aim of this study was to investigate whether we could detect D. fragilis DNA from pinworm eggs collected from routine diagnostic samples (cellophane tape) and surface-sterilised by hypochlorite. DNA was extracted from individual eggs and tested by PCR using D. fragilis- and E. vermicularis-specific primers; amplicons were sequenced for confirmation. In cellophane tape samples from 64 patients with unknown D. fragilis status we detected D. fragilis DNA in 12/238 (5%) eggs, and in a patient known to harbour D. fragilis we detected D. fragilis DNA in 39/99 (39%) eggs. The finding of D. fragilis DNA within eggs of E. vermicularis strongly supports the hypothesis of D. fragilis-transmission by pinworm and has implications for antimicrobial intervention as well as control and public health measures.",
keywords = "Former LIFE faculty, Vector, Protozoon, Nematode, Parasite, PCR, Transmission",
author = "Dennis R{\"o}ser and Peter Nejsum and Carlsgart, {Anne Josefine} and Nielsen, {Henrik Vedel} and Stensvold, {Christen Rune}",
note = "Copyright {\textcopyright} 2012. Published by Elsevier Inc.",
year = "2013",
doi = "10.1016/j.exppara.2012.10.009",
language = "English",
volume = "133",
pages = "57--61",
journal = "Experimental Parasitology",
issn = "0014-4894",
publisher = "Academic Press",
number = "1",

}

RIS

TY - JOUR

T1 - DNA of Dientamoeba fragilis detected within surface-sterilized eggs of Enterobius vermicularis

AU - Röser, Dennis

AU - Nejsum, Peter

AU - Carlsgart, Anne Josefine

AU - Nielsen, Henrik Vedel

AU - Stensvold, Christen Rune

N1 - Copyright © 2012. Published by Elsevier Inc.

PY - 2013

Y1 - 2013

N2 - With no evidence of a cyst stage, the mode of transmission of Dientamoeba fragilis, an intestinal protozoon of common occurrence and suggested pathogenicity, is incompletely known. Numerous studies have suggested that eggs of intestinal nematodes, primarily Enterobius vermicularis (pinworm), can serve as vectors for D. fragilis, although attempts to culture D. fragilis from pinworm eggs have been unsuccessful and data from epidemiological studies on D. fragilis/pinworm co-infection have been conflicting. The aim of this study was to investigate whether we could detect D. fragilis DNA from pinworm eggs collected from routine diagnostic samples (cellophane tape) and surface-sterilised by hypochlorite. DNA was extracted from individual eggs and tested by PCR using D. fragilis- and E. vermicularis-specific primers; amplicons were sequenced for confirmation. In cellophane tape samples from 64 patients with unknown D. fragilis status we detected D. fragilis DNA in 12/238 (5%) eggs, and in a patient known to harbour D. fragilis we detected D. fragilis DNA in 39/99 (39%) eggs. The finding of D. fragilis DNA within eggs of E. vermicularis strongly supports the hypothesis of D. fragilis-transmission by pinworm and has implications for antimicrobial intervention as well as control and public health measures.

AB - With no evidence of a cyst stage, the mode of transmission of Dientamoeba fragilis, an intestinal protozoon of common occurrence and suggested pathogenicity, is incompletely known. Numerous studies have suggested that eggs of intestinal nematodes, primarily Enterobius vermicularis (pinworm), can serve as vectors for D. fragilis, although attempts to culture D. fragilis from pinworm eggs have been unsuccessful and data from epidemiological studies on D. fragilis/pinworm co-infection have been conflicting. The aim of this study was to investigate whether we could detect D. fragilis DNA from pinworm eggs collected from routine diagnostic samples (cellophane tape) and surface-sterilised by hypochlorite. DNA was extracted from individual eggs and tested by PCR using D. fragilis- and E. vermicularis-specific primers; amplicons were sequenced for confirmation. In cellophane tape samples from 64 patients with unknown D. fragilis status we detected D. fragilis DNA in 12/238 (5%) eggs, and in a patient known to harbour D. fragilis we detected D. fragilis DNA in 39/99 (39%) eggs. The finding of D. fragilis DNA within eggs of E. vermicularis strongly supports the hypothesis of D. fragilis-transmission by pinworm and has implications for antimicrobial intervention as well as control and public health measures.

KW - Former LIFE faculty

KW - Vector

KW - Protozoon

KW - Nematode

KW - Parasite

KW - PCR

KW - Transmission

U2 - 10.1016/j.exppara.2012.10.009

DO - 10.1016/j.exppara.2012.10.009

M3 - Journal article

C2 - 23116599

VL - 133

SP - 57

EP - 61

JO - Experimental Parasitology

JF - Experimental Parasitology

SN - 0014-4894

IS - 1

ER -

ID: 41933555