Comparison of Faba Bean Protein Ingredients Produced Using Dry Fractionation and Isoelectric Precipitation: Techno-Functional, Nutritional and Environmental Performance
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Comparison of Faba Bean Protein Ingredients Produced Using Dry Fractionation and Isoelectric Precipitation : Techno-Functional, Nutritional and Environmental Performance. / Vogelsang-O’Dwyer, Martin; Petersen, Iben Lykke; Joehnke, Marcel Skejovic; Sørensen, Jens Christian; Bez, Juergen; Detzel, Andreas; Busch, Mirjam; Krueger, Martina; O’Mahony, James A.; Arendt, Elke K.; Zannini, Emanuele.
In: Foods, Vol. 9, No. 3, 322, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Comparison of Faba Bean Protein Ingredients Produced Using Dry Fractionation and Isoelectric Precipitation
T2 - Techno-Functional, Nutritional and Environmental Performance
AU - Vogelsang-O’Dwyer, Martin
AU - Petersen, Iben Lykke
AU - Joehnke, Marcel Skejovic
AU - Sørensen, Jens Christian
AU - Bez, Juergen
AU - Detzel, Andreas
AU - Busch, Mirjam
AU - Krueger, Martina
AU - O’Mahony, James A.
AU - Arendt, Elke K.
AU - Zannini, Emanuele
PY - 2020
Y1 - 2020
N2 - Dry fractionated faba bean protein-rich flour (FPR) produced by milling/air classification, and faba bean protein isolate (FPI) produced by acid extraction/isoelectric precipitation were compared in terms of composition, techno-functional properties, nutritional properties and environmental impacts. FPR had a lower protein content (64.1%, dry matter (DM)) compared to FPI (90.1%, DM), due to the inherent limitations of air classification. Of the two ingredients, FPR demonstrated superior functionality, including higher protein solubility (85%), compared to FPI (32%) at pH 7. Foaming capacity was higher for FPR, although foam stability was similar for both ingredients. FPR had greater gelling ability compared to FPI. The higher carbohydrate content of FPR may have contributed to this difference. An amino acid (AA) analysis revealed that both ingredients were low in sulfur-containing AAs, with FPR having a slightly higher level than FPI. The potential nutritional benefits of the aqueous process compared to the dry process used in this study were apparent in the higher in vitro protein digestibility (IVPD) and lower trypsin inhibitor activity (TIA) in FPI compared to FPR. Additionally, vicine/convicine were detected in FPR, but not in FPI. Furthermore, much lower levels of fermentable oligo-, di- and monosaccharides, and polyols (FODMAPs) were found in FPI compared to FPR. The life cycle assessment (LCA) revealed a lower environmental impact for FPR, partly due to the extra water and energy required for aqueous processing. However, in a comparison with cow’s milk protein, both FPR and FPI were shown to have considerably lower environmental impacts.
AB - Dry fractionated faba bean protein-rich flour (FPR) produced by milling/air classification, and faba bean protein isolate (FPI) produced by acid extraction/isoelectric precipitation were compared in terms of composition, techno-functional properties, nutritional properties and environmental impacts. FPR had a lower protein content (64.1%, dry matter (DM)) compared to FPI (90.1%, DM), due to the inherent limitations of air classification. Of the two ingredients, FPR demonstrated superior functionality, including higher protein solubility (85%), compared to FPI (32%) at pH 7. Foaming capacity was higher for FPR, although foam stability was similar for both ingredients. FPR had greater gelling ability compared to FPI. The higher carbohydrate content of FPR may have contributed to this difference. An amino acid (AA) analysis revealed that both ingredients were low in sulfur-containing AAs, with FPR having a slightly higher level than FPI. The potential nutritional benefits of the aqueous process compared to the dry process used in this study were apparent in the higher in vitro protein digestibility (IVPD) and lower trypsin inhibitor activity (TIA) in FPI compared to FPR. Additionally, vicine/convicine were detected in FPR, but not in FPI. Furthermore, much lower levels of fermentable oligo-, di- and monosaccharides, and polyols (FODMAPs) were found in FPI compared to FPR. The life cycle assessment (LCA) revealed a lower environmental impact for FPR, partly due to the extra water and energy required for aqueous processing. However, in a comparison with cow’s milk protein, both FPR and FPI were shown to have considerably lower environmental impacts.
KW - Antinutrients
KW - Carbon footprint
KW - Dry fractionation
KW - Faba bean
KW - FODMAPs
KW - Functional properties
KW - Isoelectric precipitation
KW - Life cycle assessment
KW - Nutrition
KW - Protein
U2 - 10.3390/foods9030322
DO - 10.3390/foods9030322
M3 - Journal article
C2 - 32168773
AN - SCOPUS:85082024979
VL - 9
JO - Foods
JF - Foods
SN - 2304-8158
IS - 3
M1 - 322
ER -
ID: 240139515