Biomimetic triblock copolymer membrane arrays: A stable template for functional membrane proteins

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Biomimetic triblock copolymer membrane arrays : A stable template for functional membrane proteins. / González-Pérez, Alfredo; Stibius, Karin B.; Vissing, Thomas; Nielsen, Claus H.; Mouritsen, Ole G.

In: Langmuir, Vol. 25, No. 18, 15.09.2009, p. 10447-10450.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

González-Pérez, A, Stibius, KB, Vissing, T, Nielsen, CH & Mouritsen, OG 2009, 'Biomimetic triblock copolymer membrane arrays: A stable template for functional membrane proteins', Langmuir, vol. 25, no. 18, pp. 10447-10450. https://doi.org/10.1021/la902417m

APA

González-Pérez, A., Stibius, K. B., Vissing, T., Nielsen, C. H., & Mouritsen, O. G. (2009). Biomimetic triblock copolymer membrane arrays: A stable template for functional membrane proteins. Langmuir, 25(18), 10447-10450. https://doi.org/10.1021/la902417m

Vancouver

González-Pérez A, Stibius KB, Vissing T, Nielsen CH, Mouritsen OG. Biomimetic triblock copolymer membrane arrays: A stable template for functional membrane proteins. Langmuir. 2009 Sep 15;25(18):10447-10450. https://doi.org/10.1021/la902417m

Author

González-Pérez, Alfredo ; Stibius, Karin B. ; Vissing, Thomas ; Nielsen, Claus H. ; Mouritsen, Ole G. / Biomimetic triblock copolymer membrane arrays : A stable template for functional membrane proteins. In: Langmuir. 2009 ; Vol. 25, No. 18. pp. 10447-10450.

Bibtex

@article{56a7cd6f6b4f4acbbca2353030ec2638,
title = "Biomimetic triblock copolymer membrane arrays: A stable template for functional membrane proteins",
abstract = "It is demonstrated that biomimetic stable triblock copolymer membrane arrays can be prepared using a scaffold containing 64 apertures of 300 μm diameter each. The membranes were made from a stock solution of block copolymers with decane as a solvent using a new deposition method. By using decane, we avoid low molecular weight solvents such as chloroform and toluene, which are strong protein denaturants. The membranes show a low ionic conductance and a long lifetime at room temperature. Contrast phase microscopy shows the presence of a polymer region delimited by a Plateau-Gibbs border similar to what is observed in black lipid membranes. The ion-channel gramicidin A was successfully incorporated into the membrane in a functional form.",
author = "Alfredo Gonz{\'a}lez-P{\'e}rez and Stibius, {Karin B.} and Thomas Vissing and Nielsen, {Claus H.} and Mouritsen, {Ole G.}",
year = "2009",
month = sep,
day = "15",
doi = "10.1021/la902417m",
language = "English",
volume = "25",
pages = "10447--10450",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "18",

}

RIS

TY - JOUR

T1 - Biomimetic triblock copolymer membrane arrays

T2 - A stable template for functional membrane proteins

AU - González-Pérez, Alfredo

AU - Stibius, Karin B.

AU - Vissing, Thomas

AU - Nielsen, Claus H.

AU - Mouritsen, Ole G.

PY - 2009/9/15

Y1 - 2009/9/15

N2 - It is demonstrated that biomimetic stable triblock copolymer membrane arrays can be prepared using a scaffold containing 64 apertures of 300 μm diameter each. The membranes were made from a stock solution of block copolymers with decane as a solvent using a new deposition method. By using decane, we avoid low molecular weight solvents such as chloroform and toluene, which are strong protein denaturants. The membranes show a low ionic conductance and a long lifetime at room temperature. Contrast phase microscopy shows the presence of a polymer region delimited by a Plateau-Gibbs border similar to what is observed in black lipid membranes. The ion-channel gramicidin A was successfully incorporated into the membrane in a functional form.

AB - It is demonstrated that biomimetic stable triblock copolymer membrane arrays can be prepared using a scaffold containing 64 apertures of 300 μm diameter each. The membranes were made from a stock solution of block copolymers with decane as a solvent using a new deposition method. By using decane, we avoid low molecular weight solvents such as chloroform and toluene, which are strong protein denaturants. The membranes show a low ionic conductance and a long lifetime at room temperature. Contrast phase microscopy shows the presence of a polymer region delimited by a Plateau-Gibbs border similar to what is observed in black lipid membranes. The ion-channel gramicidin A was successfully incorporated into the membrane in a functional form.

UR - http://www.scopus.com/inward/record.url?scp=70349929812&partnerID=8YFLogxK

U2 - 10.1021/la902417m

DO - 10.1021/la902417m

M3 - Journal article

C2 - 19735126

AN - SCOPUS:70349929812

VL - 25

SP - 10447

EP - 10450

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 18

ER -

ID: 230976831