Barley genotypic β-glucan variation combined with enzymatic modifications direct its potential as a natural ingredient in a high fiber extract
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Barley genotypic β-glucan variation combined with enzymatic modifications direct its potential as a natural ingredient in a high fiber extract. / Mikkelsen, Mette Skau; Meier, Sebastian; Jensen, Morten G; Qin, Fen; Stoica, Iuliana-Madalina; Martens, Helle Juel; Blennow, Andreas; Jespersen, Birthe P Møller.
In: Journal of Cereal Science, Vol. 75, 2017, p. 45-53.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Barley genotypic β-glucan variation combined with enzymatic modifications direct its potential as a natural ingredient in a high fiber extract
AU - Mikkelsen, Mette Skau
AU - Meier, Sebastian
AU - Jensen, Morten G
AU - Qin, Fen
AU - Stoica, Iuliana-Madalina
AU - Martens, Helle Juel
AU - Blennow, Andreas
AU - Jespersen, Birthe P Møller
PY - 2017
Y1 - 2017
N2 - β-Glucan dietary fiber rich food systems hold a great potential for improving human health. Here, we evaluate the extractability and quality of β-glucan liquid formulations from eight different barley genotypes varying in β-glucan content and molecular structure using Termamyl® SC (T), Attenuzyme® (A) and Attenuzyme® Flex (AF) amylolytic enzymes in combinations. Extracts from barley lines Lys5f, KVL408, KVL1104 and CDC Fibar exceeded 4 g β-glucan/l, providing European Food Safety Authority (EFSA) and U.S. Food and Drug Administration (FDA) recommended amounts (3 g β-glucan/day) from three portions. TAF extracts of Lys5f and KVL408 grains reached extraordinary high concentrations of 8–9 g β-glucan/l. The β-glucan molecular mass decreased with enzyme treatment T < TA < TAF due to minor lichenase side activity. Extractability was generally higher and molecular mass lower for barley lines low in triosyl/tetraosyl (DP3/DP4) ratios than for those high in DP3/DP4 ratios (Lys5f, KVL408 and KVL1104). Overall, the higher β-glucan content and structural robustness in Lys5f and KVL408 raw materials favor these in a β-glucan rich extract with potential for EFSA and FDA health and nutrition claims.
AB - β-Glucan dietary fiber rich food systems hold a great potential for improving human health. Here, we evaluate the extractability and quality of β-glucan liquid formulations from eight different barley genotypes varying in β-glucan content and molecular structure using Termamyl® SC (T), Attenuzyme® (A) and Attenuzyme® Flex (AF) amylolytic enzymes in combinations. Extracts from barley lines Lys5f, KVL408, KVL1104 and CDC Fibar exceeded 4 g β-glucan/l, providing European Food Safety Authority (EFSA) and U.S. Food and Drug Administration (FDA) recommended amounts (3 g β-glucan/day) from three portions. TAF extracts of Lys5f and KVL408 grains reached extraordinary high concentrations of 8–9 g β-glucan/l. The β-glucan molecular mass decreased with enzyme treatment T < TA < TAF due to minor lichenase side activity. Extractability was generally higher and molecular mass lower for barley lines low in triosyl/tetraosyl (DP3/DP4) ratios than for those high in DP3/DP4 ratios (Lys5f, KVL408 and KVL1104). Overall, the higher β-glucan content and structural robustness in Lys5f and KVL408 raw materials favor these in a β-glucan rich extract with potential for EFSA and FDA health and nutrition claims.
KW - Barley beta-glucans
KW - Enzymatic hydrolysis
KW - Extractability
KW - Molecular structure
U2 - 10.1016/j.jcs.2017.02.003
DO - 10.1016/j.jcs.2017.02.003
M3 - Journal article
AN - SCOPUS:85016141146
VL - 75
SP - 45
EP - 53
JO - Journal of Cereal Science
JF - Journal of Cereal Science
SN - 0733-5210
ER -
ID: 176656476