Antibiotic treatment preventing necrotising enterocolitis alters urinary and plasma metabolomes in preterm pigs

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Antibiotic treatment preventing necrotising enterocolitis alters urinary and plasma metabolomes in preterm pigs. / Jiang, Pingping; Trimigno, Alessia; Stanstrup, Jan; Khakimov, Bekzod; Viereck, Nanna; Engelsen, Søren Balling; Sangild, Per Torp; Dragsted, Lars Ove.

In: Journal of Proteome Research, Vol. 16, 2017, p. 3547-3557.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Jiang, P, Trimigno, A, Stanstrup, J, Khakimov, B, Viereck, N, Engelsen, SB, Sangild, PT & Dragsted, LO 2017, 'Antibiotic treatment preventing necrotising enterocolitis alters urinary and plasma metabolomes in preterm pigs', Journal of Proteome Research, vol. 16, pp. 3547-3557. https://doi.org/10.1021/acs.jproteome.7b00263

APA

Jiang, P., Trimigno, A., Stanstrup, J., Khakimov, B., Viereck, N., Engelsen, S. B., ... Dragsted, L. O. (2017). Antibiotic treatment preventing necrotising enterocolitis alters urinary and plasma metabolomes in preterm pigs. Journal of Proteome Research, 16, 3547-3557. https://doi.org/10.1021/acs.jproteome.7b00263

Vancouver

Jiang P, Trimigno A, Stanstrup J, Khakimov B, Viereck N, Engelsen SB et al. Antibiotic treatment preventing necrotising enterocolitis alters urinary and plasma metabolomes in preterm pigs. Journal of Proteome Research. 2017;16:3547-3557. https://doi.org/10.1021/acs.jproteome.7b00263

Author

Jiang, Pingping ; Trimigno, Alessia ; Stanstrup, Jan ; Khakimov, Bekzod ; Viereck, Nanna ; Engelsen, Søren Balling ; Sangild, Per Torp ; Dragsted, Lars Ove. / Antibiotic treatment preventing necrotising enterocolitis alters urinary and plasma metabolomes in preterm pigs. In: Journal of Proteome Research. 2017 ; Vol. 16. pp. 3547-3557.

Bibtex

@article{466332f008b241f9822b811b15a51688,
title = "Antibiotic treatment preventing necrotising enterocolitis alters urinary and plasma metabolomes in preterm pigs",
abstract = "Necrotising enterocolitis (NEC) is a serious gut inflammatory condition in premature neonates, onset and development of which depends on the gut microbiome. Attenuation of the gut microbiome by antibiotics can reduce NEC incidence and severity. However, how the antibiotics-suppressed gut microbiome affects the whole-body metabolism in NEC-sensitive premature neonates is unknown. In formula-fed preterm pigs, used as a model for preterm infants, plasma and urinary metabolomes were investigated by LC-MS and (1)H-NMR, with and without antibiotic treatment immediately after birth. While reducing the gut microbiome density and NEC lesions as previously reported, the antibiotic treatment employed in the current study affected the abundance of 44 metabolites in different metabolic pathways. In antibiotics-treated pigs, tryptophan metabolism favoured the kynurenine pathway, relative to the serotonin pathway, as shown by specific metabolites. Metabolites associated with the gut microbiome, including 3-phenyllactic acid, 4-hydroxyphenylacetic acid and phenylacetylglycine, all from phenylalanine, and three bile acids showed lower levels in the antibiotics-treated pigs where the gut microbiome was extensively attenuated. Findings in the current study warrant further investigation of metabolic and developmental consequences of antibiotic treatment in preterm neonates.",
keywords = "Antibiotics, Metabolomics, Necrotising enterocolitis, UPLC-MS, NMR, Preterm pigs",
author = "Pingping Jiang and Alessia Trimigno and Jan Stanstrup and Bekzod Khakimov and Nanna Viereck and Engelsen, {S{\o}ren Balling} and Sangild, {Per Torp} and Dragsted, {Lars Ove}",
note = "CURIS 2017 NEXS 245",
year = "2017",
doi = "10.1021/acs.jproteome.7b00263",
language = "English",
volume = "16",
pages = "3547--3557",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",

}

RIS

TY - JOUR

T1 - Antibiotic treatment preventing necrotising enterocolitis alters urinary and plasma metabolomes in preterm pigs

AU - Jiang, Pingping

AU - Trimigno, Alessia

AU - Stanstrup, Jan

AU - Khakimov, Bekzod

AU - Viereck, Nanna

AU - Engelsen, Søren Balling

AU - Sangild, Per Torp

AU - Dragsted, Lars Ove

N1 - CURIS 2017 NEXS 245

PY - 2017

Y1 - 2017

N2 - Necrotising enterocolitis (NEC) is a serious gut inflammatory condition in premature neonates, onset and development of which depends on the gut microbiome. Attenuation of the gut microbiome by antibiotics can reduce NEC incidence and severity. However, how the antibiotics-suppressed gut microbiome affects the whole-body metabolism in NEC-sensitive premature neonates is unknown. In formula-fed preterm pigs, used as a model for preterm infants, plasma and urinary metabolomes were investigated by LC-MS and (1)H-NMR, with and without antibiotic treatment immediately after birth. While reducing the gut microbiome density and NEC lesions as previously reported, the antibiotic treatment employed in the current study affected the abundance of 44 metabolites in different metabolic pathways. In antibiotics-treated pigs, tryptophan metabolism favoured the kynurenine pathway, relative to the serotonin pathway, as shown by specific metabolites. Metabolites associated with the gut microbiome, including 3-phenyllactic acid, 4-hydroxyphenylacetic acid and phenylacetylglycine, all from phenylalanine, and three bile acids showed lower levels in the antibiotics-treated pigs where the gut microbiome was extensively attenuated. Findings in the current study warrant further investigation of metabolic and developmental consequences of antibiotic treatment in preterm neonates.

AB - Necrotising enterocolitis (NEC) is a serious gut inflammatory condition in premature neonates, onset and development of which depends on the gut microbiome. Attenuation of the gut microbiome by antibiotics can reduce NEC incidence and severity. However, how the antibiotics-suppressed gut microbiome affects the whole-body metabolism in NEC-sensitive premature neonates is unknown. In formula-fed preterm pigs, used as a model for preterm infants, plasma and urinary metabolomes were investigated by LC-MS and (1)H-NMR, with and without antibiotic treatment immediately after birth. While reducing the gut microbiome density and NEC lesions as previously reported, the antibiotic treatment employed in the current study affected the abundance of 44 metabolites in different metabolic pathways. In antibiotics-treated pigs, tryptophan metabolism favoured the kynurenine pathway, relative to the serotonin pathway, as shown by specific metabolites. Metabolites associated with the gut microbiome, including 3-phenyllactic acid, 4-hydroxyphenylacetic acid and phenylacetylglycine, all from phenylalanine, and three bile acids showed lower levels in the antibiotics-treated pigs where the gut microbiome was extensively attenuated. Findings in the current study warrant further investigation of metabolic and developmental consequences of antibiotic treatment in preterm neonates.

KW - Antibiotics

KW - Metabolomics

KW - Necrotising enterocolitis

KW - UPLC-MS

KW - NMR

KW - Preterm pigs

U2 - 10.1021/acs.jproteome.7b00263

DO - 10.1021/acs.jproteome.7b00263

M3 - Journal article

C2 - 28871782

VL - 16

SP - 3547

EP - 3557

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

ER -

ID: 183010145