A novel technique for identification of Ascaris suum cohorts in pigs

Research output: Contribution to journalJournal articlepeer-review

Standard

A novel technique for identification of Ascaris suum cohorts in pigs. / Nejsum, Peter; Thamsborg, Stig Milan; Jørgensen, Claus Bøttcher; Fredholm, Merete; Roepstorff, Allan Knud.

In: Veterinary Parasitology, Vol. 154, No. 1-2, 2008, p. 171-174.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Nejsum, P, Thamsborg, SM, Jørgensen, CB, Fredholm, M & Roepstorff, AK 2008, 'A novel technique for identification of Ascaris suum cohorts in pigs', Veterinary Parasitology, vol. 154, no. 1-2, pp. 171-174. https://doi.org/10.1016/j.vetpar.2008.02.018

APA

Nejsum, P., Thamsborg, S. M., Jørgensen, C. B., Fredholm, M., & Roepstorff, A. K. (2008). A novel technique for identification of Ascaris suum cohorts in pigs. Veterinary Parasitology, 154(1-2), 171-174. https://doi.org/10.1016/j.vetpar.2008.02.018

Vancouver

Nejsum P, Thamsborg SM, Jørgensen CB, Fredholm M, Roepstorff AK. A novel technique for identification of Ascaris suum cohorts in pigs. Veterinary Parasitology. 2008;154(1-2):171-174. https://doi.org/10.1016/j.vetpar.2008.02.018

Author

Nejsum, Peter ; Thamsborg, Stig Milan ; Jørgensen, Claus Bøttcher ; Fredholm, Merete ; Roepstorff, Allan Knud. / A novel technique for identification of Ascaris suum cohorts in pigs. In: Veterinary Parasitology. 2008 ; Vol. 154, No. 1-2. pp. 171-174.

Bibtex

@article{1d549f90a1c411ddb6ae000ea68e967b,
title = "A novel technique for identification of Ascaris suum cohorts in pigs",
abstract = "The objective of the present study was to develop a fast, cheap and reliable technique for identifying different cohorts of the swine parasite, Ascaris suum. A polymerase chain reaction linked restriction fragment length polymorphism (PCR-RFLP) technique on mt-DNA was used to identify unique haplotypes of four gravid A. suum females on agarose gels after eggs were recovered from each of the worms. Each of four pigs was inoculated with 2000 embryonated eggs originating from one of the four identified Ascaris haplotypes, respectively. Ascaris larvae were isolated from the small intestine at day 14 post-infection using an agar technique. Single larvae from each pig were transferred to 96-well PCR plates and a simple DNA extraction using a worm lysis buffer was carried out and followed by the PCR-RFLP analysis. More than 100 larvae from each of the four pigs were analysed and all were found to have the same haplotype as the parental female. We conclude that unique haplotypes of female A. suum and offspring can be identified by means of PCR-RFLP on mt-DNA and suggest that this method can be used in future research on Ascaris population biology using cohorts with distinct mt-DNA profile.",
keywords = "Former LIFE faculty, Ascaris, Cohorts, Challenge infection, Host-parasite interaction, Genotypes",
author = "Peter Nejsum and Thamsborg, {Stig Milan} and J{\o}rgensen, {Claus B{\o}ttcher} and Merete Fredholm and Roepstorff, {Allan Knud}",
year = "2008",
doi = "10.1016/j.vetpar.2008.02.018",
language = "English",
volume = "154",
pages = "171--174",
journal = "Veterinary Parasitology",
issn = "0304-4017",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - A novel technique for identification of Ascaris suum cohorts in pigs

AU - Nejsum, Peter

AU - Thamsborg, Stig Milan

AU - Jørgensen, Claus Bøttcher

AU - Fredholm, Merete

AU - Roepstorff, Allan Knud

PY - 2008

Y1 - 2008

N2 - The objective of the present study was to develop a fast, cheap and reliable technique for identifying different cohorts of the swine parasite, Ascaris suum. A polymerase chain reaction linked restriction fragment length polymorphism (PCR-RFLP) technique on mt-DNA was used to identify unique haplotypes of four gravid A. suum females on agarose gels after eggs were recovered from each of the worms. Each of four pigs was inoculated with 2000 embryonated eggs originating from one of the four identified Ascaris haplotypes, respectively. Ascaris larvae were isolated from the small intestine at day 14 post-infection using an agar technique. Single larvae from each pig were transferred to 96-well PCR plates and a simple DNA extraction using a worm lysis buffer was carried out and followed by the PCR-RFLP analysis. More than 100 larvae from each of the four pigs were analysed and all were found to have the same haplotype as the parental female. We conclude that unique haplotypes of female A. suum and offspring can be identified by means of PCR-RFLP on mt-DNA and suggest that this method can be used in future research on Ascaris population biology using cohorts with distinct mt-DNA profile.

AB - The objective of the present study was to develop a fast, cheap and reliable technique for identifying different cohorts of the swine parasite, Ascaris suum. A polymerase chain reaction linked restriction fragment length polymorphism (PCR-RFLP) technique on mt-DNA was used to identify unique haplotypes of four gravid A. suum females on agarose gels after eggs were recovered from each of the worms. Each of four pigs was inoculated with 2000 embryonated eggs originating from one of the four identified Ascaris haplotypes, respectively. Ascaris larvae were isolated from the small intestine at day 14 post-infection using an agar technique. Single larvae from each pig were transferred to 96-well PCR plates and a simple DNA extraction using a worm lysis buffer was carried out and followed by the PCR-RFLP analysis. More than 100 larvae from each of the four pigs were analysed and all were found to have the same haplotype as the parental female. We conclude that unique haplotypes of female A. suum and offspring can be identified by means of PCR-RFLP on mt-DNA and suggest that this method can be used in future research on Ascaris population biology using cohorts with distinct mt-DNA profile.

KW - Former LIFE faculty

KW - Ascaris

KW - Cohorts

KW - Challenge infection

KW - Host-parasite interaction

KW - Genotypes

U2 - 10.1016/j.vetpar.2008.02.018

DO - 10.1016/j.vetpar.2008.02.018

M3 - Journal article

C2 - 18387743

VL - 154

SP - 171

EP - 174

JO - Veterinary Parasitology

JF - Veterinary Parasitology

SN - 0304-4017

IS - 1-2

ER -

ID: 8109721