A comparative study on adhesion and recovery of potential probiotic strains of Lactobacillus spp. by in vitro assay and analysis of human colon biopsies
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A comparative study on adhesion and recovery of potential probiotic strains of Lactobacillus spp. by in vitro assay and analysis of human colon biopsies. / Larsen, Nadejda Nikolajevna; Michaelsen, Kim F.; Pærregaard, Anders; Vogensen, Finn Kvist; Jakobsen, Mogens.
In: Microbiological Ecology in Health and Disease, Vol. 21, No. 2, 2009, p. 95-99.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - A comparative study on adhesion and recovery of potential probiotic strains of Lactobacillus spp. by in vitro assay and analysis of human colon biopsies
AU - Larsen, Nadejda Nikolajevna
AU - Michaelsen, Kim F.
AU - Pærregaard, Anders
AU - Vogensen, Finn Kvist
AU - Jakobsen, Mogens
PY - 2009
Y1 - 2009
N2 - Adhesion of the new Lactobacillus isolates, L. casei D12, L. casei Q85, L. casei Z11 and L. plantarum Q47, to the porcine intestinal cell line IPEC-J2 was investigated and compared to the recovery of the same bacterial strains from colon biopsies and faeces obtained from human intervention studies. Probiotic bacteria L. rhamnosus 19070, L. reuteri 12246 and L. casei F19 were used as reference strains. The new isolates exhibited low to moderate adhesion to IPEC-J2 cells in the range of 7-26%. A large variation in the recovery of strains was observed between the persons, suggesting host specificity of intestinal colonization. High correlation was shown between recovery from the different sections of the colon of the same subject, indicating consistency of bacterial colonization of the epithelium. The recovery of L. casei Z11 and L. casei Q85 was highest and comparable to the reference strains of L. rhamnosus 19070 and L. casei F19, indicating their potential to colonize the human intestine. Analysis of linear regression demonstrated poor correlation between in vitro and in vivo results, emphasizing the importance of critical evaluation of in vitro adhesion data for prediction of bacterial colonization of the gut.
AB - Adhesion of the new Lactobacillus isolates, L. casei D12, L. casei Q85, L. casei Z11 and L. plantarum Q47, to the porcine intestinal cell line IPEC-J2 was investigated and compared to the recovery of the same bacterial strains from colon biopsies and faeces obtained from human intervention studies. Probiotic bacteria L. rhamnosus 19070, L. reuteri 12246 and L. casei F19 were used as reference strains. The new isolates exhibited low to moderate adhesion to IPEC-J2 cells in the range of 7-26%. A large variation in the recovery of strains was observed between the persons, suggesting host specificity of intestinal colonization. High correlation was shown between recovery from the different sections of the colon of the same subject, indicating consistency of bacterial colonization of the epithelium. The recovery of L. casei Z11 and L. casei Q85 was highest and comparable to the reference strains of L. rhamnosus 19070 and L. casei F19, indicating their potential to colonize the human intestine. Analysis of linear regression demonstrated poor correlation between in vitro and in vivo results, emphasizing the importance of critical evaluation of in vitro adhesion data for prediction of bacterial colonization of the gut.
U2 - 10.1080/08910600902907632
DO - 10.1080/08910600902907632
M3 - Journal article
VL - 21
SP - 95
EP - 99
JO - Microbial Ecology in Health & Disease
JF - Microbial Ecology in Health & Disease
SN - 0891-060X
IS - 2
ER -
ID: 13273461