Optical micro-manipulation of mixed yeast cell populations for analyzing growth behaviour

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Optical micro-manipulation of mixed yeast cell populations for analyzing growth behaviour. / Glückstad, Jesper; Rodrigo, Peter J.; Daria, Vincent R.; Siegumfeldt, Henrik; Nissen, Peter; Arneborg, Nils.

In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 5699, 46, 21.07.2005, p. 306-312.

Research output: Contribution to journalConference articleResearchpeer-review

Harvard

Glückstad, J, Rodrigo, PJ, Daria, VR, Siegumfeldt, H, Nissen, P & Arneborg, N 2005, 'Optical micro-manipulation of mixed yeast cell populations for analyzing growth behaviour', Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 5699, 46, pp. 306-312. https://doi.org/10.1117/12.590264

APA

Glückstad, J., Rodrigo, P. J., Daria, V. R., Siegumfeldt, H., Nissen, P., & Arneborg, N. (2005). Optical micro-manipulation of mixed yeast cell populations for analyzing growth behaviour. Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 5699, 306-312. [46]. https://doi.org/10.1117/12.590264

Vancouver

Glückstad J, Rodrigo PJ, Daria VR, Siegumfeldt H, Nissen P, Arneborg N. Optical micro-manipulation of mixed yeast cell populations for analyzing growth behaviour. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2005 Jul 21;5699:306-312. 46. https://doi.org/10.1117/12.590264

Author

Glückstad, Jesper ; Rodrigo, Peter J. ; Daria, Vincent R. ; Siegumfeldt, Henrik ; Nissen, Peter ; Arneborg, Nils. / Optical micro-manipulation of mixed yeast cell populations for analyzing growth behaviour. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2005 ; Vol. 5699. pp. 306-312.

Bibtex

@inproceedings{48853698ebfe46fcaae3a28e68cde0c1,
title = "Optical micro-manipulation of mixed yeast cell populations for analyzing growth behaviour",
abstract = "We use spatially sculptured light for user-interactive micromanipulation of mixed yeast cell populations to analyze growth behavioural patterns. There is negligible absorption in the near-infrared region of the light spectrum making it suitable for direct manipulation of individual cells in a growing population. Rather than using a single-beam optical trap, multiple cells are manipulated using a system based on the Generalized Phase Contrast (GPC) method, which allows arbitrary trapping configurations i.e. control over the number of traps, and the size/shape of each trap. This enables the cells to be selectively trapped in all three-dimensions (3D) and manipulated in real-time while under direct observation. Here, we impose controlled experiments using these multiple 3D optical traps to show the alteration of growth patterns in mixed cultures of Saccharomyces cerevisiae and Hanseniaspora uvarum experiencing spatially constrained conditions.",
keywords = "Generalized phase contrast, Manipulation of living cells, Real-time multi-beam optical trapping",
author = "Jesper Gl{\"u}ckstad and Rodrigo, {Peter J.} and Daria, {Vincent R.} and Henrik Siegumfeldt and Peter Nissen and Nils Arneborg",
year = "2005",
month = "7",
day = "21",
doi = "10.1117/12.590264",
language = "English",
volume = "5699",
pages = "306--312",
journal = "Progress in Biomedical Optics and Imaging",
issn = "1605-7422",
publisher = "S P I E - International Society for Optical Engineering",
note = "Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III ; Conference date: 24-01-2005 Through 27-01-2005",

}

RIS

TY - GEN

T1 - Optical micro-manipulation of mixed yeast cell populations for analyzing growth behaviour

AU - Glückstad, Jesper

AU - Rodrigo, Peter J.

AU - Daria, Vincent R.

AU - Siegumfeldt, Henrik

AU - Nissen, Peter

AU - Arneborg, Nils

PY - 2005/7/21

Y1 - 2005/7/21

N2 - We use spatially sculptured light for user-interactive micromanipulation of mixed yeast cell populations to analyze growth behavioural patterns. There is negligible absorption in the near-infrared region of the light spectrum making it suitable for direct manipulation of individual cells in a growing population. Rather than using a single-beam optical trap, multiple cells are manipulated using a system based on the Generalized Phase Contrast (GPC) method, which allows arbitrary trapping configurations i.e. control over the number of traps, and the size/shape of each trap. This enables the cells to be selectively trapped in all three-dimensions (3D) and manipulated in real-time while under direct observation. Here, we impose controlled experiments using these multiple 3D optical traps to show the alteration of growth patterns in mixed cultures of Saccharomyces cerevisiae and Hanseniaspora uvarum experiencing spatially constrained conditions.

AB - We use spatially sculptured light for user-interactive micromanipulation of mixed yeast cell populations to analyze growth behavioural patterns. There is negligible absorption in the near-infrared region of the light spectrum making it suitable for direct manipulation of individual cells in a growing population. Rather than using a single-beam optical trap, multiple cells are manipulated using a system based on the Generalized Phase Contrast (GPC) method, which allows arbitrary trapping configurations i.e. control over the number of traps, and the size/shape of each trap. This enables the cells to be selectively trapped in all three-dimensions (3D) and manipulated in real-time while under direct observation. Here, we impose controlled experiments using these multiple 3D optical traps to show the alteration of growth patterns in mixed cultures of Saccharomyces cerevisiae and Hanseniaspora uvarum experiencing spatially constrained conditions.

KW - Generalized phase contrast

KW - Manipulation of living cells

KW - Real-time multi-beam optical trapping

UR - http://www.scopus.com/inward/record.url?scp=21844465640&partnerID=8YFLogxK

U2 - 10.1117/12.590264

DO - 10.1117/12.590264

M3 - Conference article

AN - SCOPUS:21844465640

VL - 5699

SP - 306

EP - 312

JO - Progress in Biomedical Optics and Imaging

JF - Progress in Biomedical Optics and Imaging

SN - 1605-7422

M1 - 46

T2 - Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III

Y2 - 24 January 2005 through 27 January 2005

ER -

ID: 225839121