Nutrient Restriction has Limited Short-Term Effects on Gut, Immunity, and Brain Development in Preterm Pigs

Research output: Contribution to journalJournal articleResearchpeer-review

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Nutrient Restriction has Limited Short-Term Effects on Gut, Immunity, and Brain Development in Preterm Pigs. / Ahnfeldt, Agnethe May; Bæk, Ole; Hui, Yan; Nielsen, Charlotte Holme; Obelitz-Ryom, Karina; Busk-Anderson, Tilla; Ruge, Anne; Holst, Jens Juul; Rudloff, Silvia; Burrin, Douglas; Nguyen, Duc Ninh; Nielsen, Dennis Sandris; Zachariassen, Gitte; Bering, Stine Brandt; Thymann, Thomas; Sangild, Per Torp.

In: The Journal of Nutrition, 18.02.2020.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Ahnfeldt, AM, Bæk, O, Hui, Y, Nielsen, CH, Obelitz-Ryom, K, Busk-Anderson, T, Ruge, A, Holst, JJ, Rudloff, S, Burrin, D, Nguyen, DN, Nielsen, DS, Zachariassen, G, Bering, SB, Thymann, T & Sangild, PT 2020, 'Nutrient Restriction has Limited Short-Term Effects on Gut, Immunity, and Brain Development in Preterm Pigs', The Journal of Nutrition. https://doi.org/10.1093/jn/nxaa030

APA

Ahnfeldt, A. M., Bæk, O., Hui, Y., Nielsen, C. H., Obelitz-Ryom, K., Busk-Anderson, T., ... Sangild, P. T. (2020). Nutrient Restriction has Limited Short-Term Effects on Gut, Immunity, and Brain Development in Preterm Pigs. The Journal of Nutrition. https://doi.org/10.1093/jn/nxaa030

Vancouver

Ahnfeldt AM, Bæk O, Hui Y, Nielsen CH, Obelitz-Ryom K, Busk-Anderson T et al. Nutrient Restriction has Limited Short-Term Effects on Gut, Immunity, and Brain Development in Preterm Pigs. The Journal of Nutrition. 2020 Feb 18. https://doi.org/10.1093/jn/nxaa030

Author

Ahnfeldt, Agnethe May ; Bæk, Ole ; Hui, Yan ; Nielsen, Charlotte Holme ; Obelitz-Ryom, Karina ; Busk-Anderson, Tilla ; Ruge, Anne ; Holst, Jens Juul ; Rudloff, Silvia ; Burrin, Douglas ; Nguyen, Duc Ninh ; Nielsen, Dennis Sandris ; Zachariassen, Gitte ; Bering, Stine Brandt ; Thymann, Thomas ; Sangild, Per Torp. / Nutrient Restriction has Limited Short-Term Effects on Gut, Immunity, and Brain Development in Preterm Pigs. In: The Journal of Nutrition. 2020.

Bibtex

@article{2047d46dfaf74aad8697e0edeb54f42f,
title = "Nutrient Restriction has Limited Short-Term Effects on Gut, Immunity, and Brain Development in Preterm Pigs",
abstract = "BACKGROUND: Extrauterine growth restriction (EUGR) in preterm infants is associated with higher morbidity and impaired neurodevelopment. Early nutrition support may prevent EUGR in preterm infants, but it is not known if this improves organ development and brain function in the short and long term.OBJECTIVE: Using pigs as models for infants, we hypothesized that diet-induced EUGR impairs gut, immunity, and brain development in preterm neonates during the first weeks after birth.METHODS: Forty-four preterm caesarean-delivered pigs (Danish Landrace × Large White × Duroc, birth weight 975 ± 235 g, male:female ratio 23:21) from 2 sows were fed increasing volumes [32-180 mL/(kg·d)] of dilute bovine milk (EUGR group) or the same diet fortified with powdered bovine colostrum for 19 d (CONT group, 50-100{\%} higher protein and energy intake than the EUGR group).RESULTS: The EUGR pigs showed reduced body growth (-39{\%}, P < 0.01), lower plasma albumin, phosphate, and creatine kinase concentrations (-35 to 14{\%}, P < 0.05), increased cortisol and free iron concentrations (+130 to 700{\%}, P < 0.05), and reduced relative weights of the intestine, liver, and spleen (-38 to 19{\%}, all P < 0.05). The effects of EUGR on gut structure, function, microbiota, and systemic immunity were marginal, although EUGR temporarily increased type 1 helper T cell (Th1) activity (e.g. more blood T cells and higher Th1-related cytokine concentrations on day 8) and reduced colon nutrient fermentation (lower SCFA concentration; -45{\%}, P < 0.01). Further, EUGR pigs showed increased relative brain weights (+19{\%}, P < 0.01), however, memory and learning, as tested in a spatial T-maze, were not affected.CONCLUSION: Most of the measured organ growth, and digestive, immune, and brain functions showed limited effects of diet-induced EUGR in preterm pigs during the first weeks after birth. Likewise, preterm infants may show remarkable physiological adaptation to deficient nutrient supply during the first weeks of life although early life malnutrition may exert negative consequences later.",
author = "Ahnfeldt, {Agnethe May} and Ole B{\ae}k and Yan Hui and Nielsen, {Charlotte Holme} and Karina Obelitz-Ryom and Tilla Busk-Anderson and Anne Ruge and Holst, {Jens Juul} and Silvia Rudloff and Douglas Burrin and Nguyen, {Duc Ninh} and Nielsen, {Dennis Sandris} and Gitte Zachariassen and Bering, {Stine Brandt} and Thomas Thymann and Sangild, {Per Torp}",
note = "Copyright {\circledC} The Author(s) 2020.",
year = "2020",
month = "2",
day = "18",
doi = "10.1093/jn/nxaa030",
language = "English",
journal = "Journal of Nutrition",
issn = "0022-3166",
publisher = "American Society for Nutrition",

}

RIS

TY - JOUR

T1 - Nutrient Restriction has Limited Short-Term Effects on Gut, Immunity, and Brain Development in Preterm Pigs

AU - Ahnfeldt, Agnethe May

AU - Bæk, Ole

AU - Hui, Yan

AU - Nielsen, Charlotte Holme

AU - Obelitz-Ryom, Karina

AU - Busk-Anderson, Tilla

AU - Ruge, Anne

AU - Holst, Jens Juul

AU - Rudloff, Silvia

AU - Burrin, Douglas

AU - Nguyen, Duc Ninh

AU - Nielsen, Dennis Sandris

AU - Zachariassen, Gitte

AU - Bering, Stine Brandt

AU - Thymann, Thomas

AU - Sangild, Per Torp

N1 - Copyright © The Author(s) 2020.

PY - 2020/2/18

Y1 - 2020/2/18

N2 - BACKGROUND: Extrauterine growth restriction (EUGR) in preterm infants is associated with higher morbidity and impaired neurodevelopment. Early nutrition support may prevent EUGR in preterm infants, but it is not known if this improves organ development and brain function in the short and long term.OBJECTIVE: Using pigs as models for infants, we hypothesized that diet-induced EUGR impairs gut, immunity, and brain development in preterm neonates during the first weeks after birth.METHODS: Forty-four preterm caesarean-delivered pigs (Danish Landrace × Large White × Duroc, birth weight 975 ± 235 g, male:female ratio 23:21) from 2 sows were fed increasing volumes [32-180 mL/(kg·d)] of dilute bovine milk (EUGR group) or the same diet fortified with powdered bovine colostrum for 19 d (CONT group, 50-100% higher protein and energy intake than the EUGR group).RESULTS: The EUGR pigs showed reduced body growth (-39%, P < 0.01), lower plasma albumin, phosphate, and creatine kinase concentrations (-35 to 14%, P < 0.05), increased cortisol and free iron concentrations (+130 to 700%, P < 0.05), and reduced relative weights of the intestine, liver, and spleen (-38 to 19%, all P < 0.05). The effects of EUGR on gut structure, function, microbiota, and systemic immunity were marginal, although EUGR temporarily increased type 1 helper T cell (Th1) activity (e.g. more blood T cells and higher Th1-related cytokine concentrations on day 8) and reduced colon nutrient fermentation (lower SCFA concentration; -45%, P < 0.01). Further, EUGR pigs showed increased relative brain weights (+19%, P < 0.01), however, memory and learning, as tested in a spatial T-maze, were not affected.CONCLUSION: Most of the measured organ growth, and digestive, immune, and brain functions showed limited effects of diet-induced EUGR in preterm pigs during the first weeks after birth. Likewise, preterm infants may show remarkable physiological adaptation to deficient nutrient supply during the first weeks of life although early life malnutrition may exert negative consequences later.

AB - BACKGROUND: Extrauterine growth restriction (EUGR) in preterm infants is associated with higher morbidity and impaired neurodevelopment. Early nutrition support may prevent EUGR in preterm infants, but it is not known if this improves organ development and brain function in the short and long term.OBJECTIVE: Using pigs as models for infants, we hypothesized that diet-induced EUGR impairs gut, immunity, and brain development in preterm neonates during the first weeks after birth.METHODS: Forty-four preterm caesarean-delivered pigs (Danish Landrace × Large White × Duroc, birth weight 975 ± 235 g, male:female ratio 23:21) from 2 sows were fed increasing volumes [32-180 mL/(kg·d)] of dilute bovine milk (EUGR group) or the same diet fortified with powdered bovine colostrum for 19 d (CONT group, 50-100% higher protein and energy intake than the EUGR group).RESULTS: The EUGR pigs showed reduced body growth (-39%, P < 0.01), lower plasma albumin, phosphate, and creatine kinase concentrations (-35 to 14%, P < 0.05), increased cortisol and free iron concentrations (+130 to 700%, P < 0.05), and reduced relative weights of the intestine, liver, and spleen (-38 to 19%, all P < 0.05). The effects of EUGR on gut structure, function, microbiota, and systemic immunity were marginal, although EUGR temporarily increased type 1 helper T cell (Th1) activity (e.g. more blood T cells and higher Th1-related cytokine concentrations on day 8) and reduced colon nutrient fermentation (lower SCFA concentration; -45%, P < 0.01). Further, EUGR pigs showed increased relative brain weights (+19%, P < 0.01), however, memory and learning, as tested in a spatial T-maze, were not affected.CONCLUSION: Most of the measured organ growth, and digestive, immune, and brain functions showed limited effects of diet-induced EUGR in preterm pigs during the first weeks after birth. Likewise, preterm infants may show remarkable physiological adaptation to deficient nutrient supply during the first weeks of life although early life malnutrition may exert negative consequences later.

U2 - 10.1093/jn/nxaa030

DO - 10.1093/jn/nxaa030

M3 - Journal article

C2 - 32069355

JO - Journal of Nutrition

JF - Journal of Nutrition

SN - 0022-3166

ER -

ID: 236318130