Mode of delivery shapes gut colonization pattern and modulates regulatory immunity in mice

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Mode of delivery shapes gut colonization pattern and modulates regulatory immunity in mice. / Hansen, Camilla Hartmann Friis; Andersen, Line Sidsel Fisker; Krych, Lukasz; Metzdorff, Stine Broeng; Hasselby, Jane Preuss; Skov, Søren; Nielsen, Dennis Sandris; Buschard, Karsten; Hansen, Lars H.; Hansen, Axel Kornerup.

In: Journal of Immunology, Vol. 193, No. 3, 2014, p. 1213-1222.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Hansen, CHF, Andersen, LSF, Krych, L, Metzdorff, SB, Hasselby, JP, Skov, S, Nielsen, DS, Buschard, K, Hansen, LH & Hansen, AK 2014, 'Mode of delivery shapes gut colonization pattern and modulates regulatory immunity in mice', Journal of Immunology, vol. 193, no. 3, pp. 1213-1222. https://doi.org/10.4049/jimmunol.1400085

APA

Hansen, C. H. F., Andersen, L. S. F., Krych, L., Metzdorff, S. B., Hasselby, J. P., Skov, S., ... Hansen, A. K. (2014). Mode of delivery shapes gut colonization pattern and modulates regulatory immunity in mice. Journal of Immunology, 193(3), 1213-1222. https://doi.org/10.4049/jimmunol.1400085

Vancouver

Hansen CHF, Andersen LSF, Krych L, Metzdorff SB, Hasselby JP, Skov S et al. Mode of delivery shapes gut colonization pattern and modulates regulatory immunity in mice. Journal of Immunology. 2014;193(3):1213-1222. https://doi.org/10.4049/jimmunol.1400085

Author

Hansen, Camilla Hartmann Friis ; Andersen, Line Sidsel Fisker ; Krych, Lukasz ; Metzdorff, Stine Broeng ; Hasselby, Jane Preuss ; Skov, Søren ; Nielsen, Dennis Sandris ; Buschard, Karsten ; Hansen, Lars H. ; Hansen, Axel Kornerup. / Mode of delivery shapes gut colonization pattern and modulates regulatory immunity in mice. In: Journal of Immunology. 2014 ; Vol. 193, No. 3. pp. 1213-1222.

Bibtex

@article{af9d1174174a485b86111b11325338de,
title = "Mode of delivery shapes gut colonization pattern and modulates regulatory immunity in mice",
abstract = "Delivery mode has been associated with long-term changes in gut microbiota composition and more recently also with changes in the immune system. This has further been suggested to link Cesarean section (C-section) with an increased risk for development of immune-mediated diseases such as type 1 diabetes. In this study, we demonstrate that both C-section and cross-fostering with a genetically distinct strain influence the gut microbiota composition and immune key markers in mice. Gut microbiota profiling by denaturing gradient gel electrophoresis and 454/FLX-based 16S rRNA gene amplicon sequencing revealed that mice born by C-section had a distinct bacterial profile at weaning characterized by higher abundance of Bacteroides and Lachnospiraceae, and less Rikenellaceae and Ruminococcus. No clustering according to delivery method as determined by principal component analysis of denaturing gradient gel electrophoresis profiles was evident in adult mice. However, the adult C-section-born mice had lower proportions of Foxp3(+) regulatory T cells, tolerogenic CD103(+) dendritic cells, and less Il10 gene expression in mesenteric lymph nodes and spleens. This demonstrates long-term systemic effect on the regulatory immune system that was also evident in NOD mice, a model of type 1 diabetes, born by C-section. However, no effect of delivery mode was seen on diabetes incidence or insulitis development. In conclusion, the first exposure to microorganisms seems to be crucial for the early life gut microbiota and priming of regulatory immune system in mice, and mode of delivery strongly influences this.",
keywords = "Adaptive Immunity, Animals, Bacteroides, Cesarean Section, Clostridium, Diabetes Mellitus, Experimental, Female, Intestines, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Inbred NOD, Microbiota, Mucous Membrane, Ruminococcus, T-Lymphocytes, Regulatory",
author = "Hansen, {Camilla Hartmann Friis} and Andersen, {Line Sidsel Fisker} and Lukasz Krych and Metzdorff, {Stine Broeng} and Hasselby, {Jane Preuss} and S{\o}ren Skov and Nielsen, {Dennis Sandris} and Karsten Buschard and Hansen, {Lars H.} and Hansen, {Axel Kornerup}",
note = "Copyright {\circledC} 2014 by The American Association of Immunologists, Inc.",
year = "2014",
doi = "10.4049/jimmunol.1400085",
language = "English",
volume = "193",
pages = "1213--1222",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "3",

}

RIS

TY - JOUR

T1 - Mode of delivery shapes gut colonization pattern and modulates regulatory immunity in mice

AU - Hansen, Camilla Hartmann Friis

AU - Andersen, Line Sidsel Fisker

AU - Krych, Lukasz

AU - Metzdorff, Stine Broeng

AU - Hasselby, Jane Preuss

AU - Skov, Søren

AU - Nielsen, Dennis Sandris

AU - Buschard, Karsten

AU - Hansen, Lars H.

AU - Hansen, Axel Kornerup

N1 - Copyright © 2014 by The American Association of Immunologists, Inc.

PY - 2014

Y1 - 2014

N2 - Delivery mode has been associated with long-term changes in gut microbiota composition and more recently also with changes in the immune system. This has further been suggested to link Cesarean section (C-section) with an increased risk for development of immune-mediated diseases such as type 1 diabetes. In this study, we demonstrate that both C-section and cross-fostering with a genetically distinct strain influence the gut microbiota composition and immune key markers in mice. Gut microbiota profiling by denaturing gradient gel electrophoresis and 454/FLX-based 16S rRNA gene amplicon sequencing revealed that mice born by C-section had a distinct bacterial profile at weaning characterized by higher abundance of Bacteroides and Lachnospiraceae, and less Rikenellaceae and Ruminococcus. No clustering according to delivery method as determined by principal component analysis of denaturing gradient gel electrophoresis profiles was evident in adult mice. However, the adult C-section-born mice had lower proportions of Foxp3(+) regulatory T cells, tolerogenic CD103(+) dendritic cells, and less Il10 gene expression in mesenteric lymph nodes and spleens. This demonstrates long-term systemic effect on the regulatory immune system that was also evident in NOD mice, a model of type 1 diabetes, born by C-section. However, no effect of delivery mode was seen on diabetes incidence or insulitis development. In conclusion, the first exposure to microorganisms seems to be crucial for the early life gut microbiota and priming of regulatory immune system in mice, and mode of delivery strongly influences this.

AB - Delivery mode has been associated with long-term changes in gut microbiota composition and more recently also with changes in the immune system. This has further been suggested to link Cesarean section (C-section) with an increased risk for development of immune-mediated diseases such as type 1 diabetes. In this study, we demonstrate that both C-section and cross-fostering with a genetically distinct strain influence the gut microbiota composition and immune key markers in mice. Gut microbiota profiling by denaturing gradient gel electrophoresis and 454/FLX-based 16S rRNA gene amplicon sequencing revealed that mice born by C-section had a distinct bacterial profile at weaning characterized by higher abundance of Bacteroides and Lachnospiraceae, and less Rikenellaceae and Ruminococcus. No clustering according to delivery method as determined by principal component analysis of denaturing gradient gel electrophoresis profiles was evident in adult mice. However, the adult C-section-born mice had lower proportions of Foxp3(+) regulatory T cells, tolerogenic CD103(+) dendritic cells, and less Il10 gene expression in mesenteric lymph nodes and spleens. This demonstrates long-term systemic effect on the regulatory immune system that was also evident in NOD mice, a model of type 1 diabetes, born by C-section. However, no effect of delivery mode was seen on diabetes incidence or insulitis development. In conclusion, the first exposure to microorganisms seems to be crucial for the early life gut microbiota and priming of regulatory immune system in mice, and mode of delivery strongly influences this.

KW - Adaptive Immunity

KW - Animals

KW - Bacteroides

KW - Cesarean Section

KW - Clostridium

KW - Diabetes Mellitus, Experimental

KW - Female

KW - Intestines

KW - Male

KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, Inbred C57BL

KW - Mice, Inbred NOD

KW - Microbiota

KW - Mucous Membrane

KW - Ruminococcus

KW - T-Lymphocytes, Regulatory

U2 - 10.4049/jimmunol.1400085

DO - 10.4049/jimmunol.1400085

M3 - Journal article

C2 - 24951818

VL - 193

SP - 1213

EP - 1222

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 3

ER -

ID: 124556790