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Effect of scatterer and absorber on fluorescence

Educational level:Master thesis
Target group:MSc Food Science and Technology
Main area:Chemometrics and Analytical Technology
Ingredients and Dairy Technology
Project description:

It is known that the behaviour of fluorescence excitation-emission matrices (EEMs) is highly influenced by the local environment of the fluorophores. There is a recent study where they claim to have been able to model how a scattering interferent and an absorber would effect the subsequent excitation-emission matrix. In this project, we would like to investigate if their findings actually hold in the real world. The samples will contain 3-4 fluorophores with distint fluorescence spectra, 1 scatterer and 1-2 absorbers. These are to be mixed according to an experimental design, and the effect of the scatterer and absorber(s) on the EEMs will be studied by PARAFAC and visual assessment.

Please contact Åsmund Rinnan or Marta Bevilaqua (marta@food.ku.dk)

Keywords:Fluorescence, Chemometrics, Interferents
Supervisor(s): Åsmund Rinnan and Marta Bevilaqua
Email:aar@food.ku.dk
Last edited:28-01-2019