The quorum-sensing molecule 2-phenylethanol impaired conidial germination, hyphal membrane integrity and growth of Penicillium expansum and Penicillium nordicum
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The quorum-sensing molecule 2-phenylethanol impaired conidial germination, hyphal membrane integrity and growth of Penicillium expansum and Penicillium nordicum. / Huang, C.; Qian, Y.; Viana, T.; Siegumfeldt, H.; Arneborg, N.; Larsen, N.; Jespersen, L.
I: Journal of Applied Microbiology, Bind 129, Nr. 2, 2020, s. 278-286.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The quorum-sensing molecule 2-phenylethanol impaired conidial germination, hyphal membrane integrity and growth of Penicillium expansum and Penicillium nordicum
AU - Huang, C.
AU - Qian, Y.
AU - Viana, T.
AU - Siegumfeldt, H.
AU - Arneborg, N.
AU - Larsen, N.
AU - Jespersen, L.
PY - 2020
Y1 - 2020
N2 - Aims: The aim of the study was to investigate the antifungal effects of a quorum sensing-molecule, 2-phenylethanol, against the food spoilage moulds Penicillium expansum and Penicillium nordicum. Methods and Results: Conidial germination of the tested Penicillium spp. (three strains in total) were inhibited by treatments with 2-phenylethanol in a concentration-dependent manner. Germinated conidia was significantly reduced from 4·4–16·7% at 7·5 mmol l−1 and completely inhibited at 15 mmol l−1 2-phenylethanol. Integrity of conidial cell membranes was unaffected by 2-phenylethanol resulting in reversible inhibition pattern of germination. In contrast, membrane permeability of actively growing hyphae was severely compromised, showing 63·5 – 75·7% membrane damage upon treatment with 15 mmol l−1 2-phenylethanol. The overall inhibitory effect of 2-phenylethanol on colony development and growth of P. expansum and P. nordicum was additionally confirmed. Conclusions: 2-phenylethanol inhibits conidial germination and growth of P. expansum and P. nordicum in a nonlethal, reversible and concentration-dependent manner. Significance and Impact of the Study: The study indicates that 2-phenylethanol can find potential application as an antifungal agent for biological control of moulds in the food industry.
AB - Aims: The aim of the study was to investigate the antifungal effects of a quorum sensing-molecule, 2-phenylethanol, against the food spoilage moulds Penicillium expansum and Penicillium nordicum. Methods and Results: Conidial germination of the tested Penicillium spp. (three strains in total) were inhibited by treatments with 2-phenylethanol in a concentration-dependent manner. Germinated conidia was significantly reduced from 4·4–16·7% at 7·5 mmol l−1 and completely inhibited at 15 mmol l−1 2-phenylethanol. Integrity of conidial cell membranes was unaffected by 2-phenylethanol resulting in reversible inhibition pattern of germination. In contrast, membrane permeability of actively growing hyphae was severely compromised, showing 63·5 – 75·7% membrane damage upon treatment with 15 mmol l−1 2-phenylethanol. The overall inhibitory effect of 2-phenylethanol on colony development and growth of P. expansum and P. nordicum was additionally confirmed. Conclusions: 2-phenylethanol inhibits conidial germination and growth of P. expansum and P. nordicum in a nonlethal, reversible and concentration-dependent manner. Significance and Impact of the Study: The study indicates that 2-phenylethanol can find potential application as an antifungal agent for biological control of moulds in the food industry.
KW - 2-Phenylethanol
KW - cell membrane integrity
KW - conidial germination
KW - mould inhibition
KW - Penicillium spp.
U2 - 10.1111/jam.14621
DO - 10.1111/jam.14621
M3 - Journal article
C2 - 32097516
AN - SCOPUS:85081734729
VL - 129
SP - 278
EP - 286
JO - Proceedings of the Society for Applied Bacteriology
JF - Proceedings of the Society for Applied Bacteriology
SN - 0266-8254
IS - 2
ER -
ID: 240191600