Saccharomyces cerevisiae does not undergo a quorum sensing-dependent switch of budding pattern

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Saccharomyces cerevisiae can alter its morphology to a filamentous form associated with unipolar budding in response to environmental stressors. Induction of filamentous growth is suggested under nitrogen deficiency in response to alcoholic signalling molecules through quorum sensing. To investigate this further, we analysed the budding pattern of S. cerevisiae cells over time under low nitrogen conditions while concurrently measuring cell density and extracellular metabolite concentration. We found that the proportion of cells displaying unipolar budding increased between local cell densities of 4.8 × 106 and 5.3 × 107 cells/ml. This increase in unipolar budding was not reproduced with cells growing at the critical cell density and in conditioned media. Growth under high nitrogen conditions also resulted in increased unipolar budding between local cell densities of 5.2 × 106 and 8.2 × 107 cells/ml, but with differences in metabolite concentration compared to low nitrogen conditions. Neither cell density, metabolite concentration, nor nitrogen deficiency were therefore sufficient to increase unipolar budding. Therefore, by using the budding pattern as an early indicator of filamentous growth, our results suggest that quorum sensing may not control the switch of budding behaviour in S. cerevisiae. Only a high concentration of the putative signalling molecule, 2-phenylethanol, resulted in an increase in unipolar budding. However, this concentration was not physiologically relevant, suggesting toxicity rather than a known quorum sensing mechanism.

OriginalsprogEngelsk
Artikelnummer8738
TidsskriftScientific Reports
Vol/bind12
Antal sider17
ISSN2045-2322
DOI
StatusUdgivet - 2022

Bibliografisk note

Funding Information:
This research was supported by the Faculty of Veterinary and Agricultural Science at the University of Melbourne and an Australian Government Research Training Program (RTP) Scholarship. We are grateful to Nadia Devargue, Chu Chu Huang and Sebastian Bech-Terkilsen from the University of Copenhagen for assistance in the laboratory, and Franciscus Winfried J van der Berg from the University of Copenhagen for allowing us use of an oCelloScopeTM. Figures 1 , 2 , 6 and 7 were created with BioRender.com.

Funding Information:
This research was supported by the Faculty of Veterinary and Agricultural Science at the University of Melbourne and an Australian Government Research Training Program (RTP) Scholarship. We are grateful to Nadia Devargue, Chu Chu Huang and Sebastian Bech-Terkilsen from the University of Copenhagen for assistance in the laboratory, and Franciscus Winfried J van der Berg from the University of Copenhagen for allowing us use of an oCelloScope. Figures , , and were created with BioRender.com. TM

Publisher Copyright:
© 2022, The Author(s).

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