Flow cytometric detection of wild yeast in lager breweries

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

A flow cytometric method for detection of wild yeast infections in breweries is reported. It is based on selective enrichment in Malt extract Yeast extract Glucose Peptone broth (MYGP) at 37 degrees C and in MYGP with 200 ppm CuSO4 at 25 degrees C, staining with a fluorochrome precursor and flow cytometry. In experiments with several types of wild yeast isolated from breweries and two different strains of lager yeast it has been possible to detect one wild yeast per 10(6) culture yeast after 48-72 h of incubation and, in some cases, after 24 h.

OriginalsprogEngelsk
TidsskriftInternational Journal of Food Microbiology
Vol/bind17
Udgave nummer4
Sider (fra-til)321-328
Antal sider8
ISSN0168-1605
DOI
StatusUdgivet - 1993
Eksternt udgivetJa

ID: 131532094